Endothelial Cell Death and Intimal Foam Cell Accumulation in the Coronary Artery of Infected Hypercholesterolemic Minipigs
(2013) In Journal of Cardiovascular Translational Research 6(4). p.579-587- Abstract
- Apoptosis of endothelial cells (ECs) has been suggested to play a role in atherosclerosis. We studied the synergism of hypercholesterolemia with Chlamydia pneumoniae and influenza virus infections on EC morphology and intimal changes in a minipig model. The coronary artery was excised at euthanasia (19 weeks of age) and serial sections were processed for the detection of EC apoptosis, histology, and transmission electron microscopy (TEM) studies. There was a significantly higher number of TUNEL-positive ECs in infected compared to noninfected groups [0.2942 % (interquartile ranges (IR), 0.2941; n = 26) versus 0 % (IR, 0; n = 12), p < 0.01]. Caspase-3 staining was negative. Cholesterol diet together with infections induced widening of... (More)
- Apoptosis of endothelial cells (ECs) has been suggested to play a role in atherosclerosis. We studied the synergism of hypercholesterolemia with Chlamydia pneumoniae and influenza virus infections on EC morphology and intimal changes in a minipig model. The coronary artery was excised at euthanasia (19 weeks of age) and serial sections were processed for the detection of EC apoptosis, histology, and transmission electron microscopy (TEM) studies. There was a significantly higher number of TUNEL-positive ECs in infected compared to noninfected groups [0.2942 % (interquartile ranges (IR), 0.2941; n = 26) versus 0 % (IR, 0; n = 12), p < 0.01]. Caspase-3 staining was negative. Cholesterol diet together with infections induced widening of the subendothelial space and appearance of increased numbers of foam cells. TEM revealed degenerative changes in cytoplasmic organelles and signs of EC necrosis. In conclusion, infection leads to an increase in coronary EC death and seems to exacerbate cholesterol-induced intimal thickening and foam cell accumulation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4042806
- author
- Birck, Malene M. ; Saraste, Antti ; Hyttel, Poul ; Odermarsky, Michal LU ; Liuba, Petru LU ; Saukko, Pekka ; Hansen, Axel K. and Pesonen, Erkki LU
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Atherosclerosis, Endothelial cells, Infection, Pig model, Cell death
- in
- Journal of Cardiovascular Translational Research
- volume
- 6
- issue
- 4
- pages
- 579 - 587
- publisher
- Springer
- external identifiers
-
- wos:000321868500014
- scopus:84880513521
- pmid:23580230
- ISSN
- 1937-5395
- DOI
- 10.1007/s12265-013-9463-2
- language
- English
- LU publication?
- no
- id
- 64557a55-f55c-42b5-8c1e-39bf8e5adeb9 (old id 4042806)
- date added to LUP
- 2016-04-01 11:02:25
- date last changed
- 2022-04-28 03:53:19
@article{64557a55-f55c-42b5-8c1e-39bf8e5adeb9, abstract = {{Apoptosis of endothelial cells (ECs) has been suggested to play a role in atherosclerosis. We studied the synergism of hypercholesterolemia with Chlamydia pneumoniae and influenza virus infections on EC morphology and intimal changes in a minipig model. The coronary artery was excised at euthanasia (19 weeks of age) and serial sections were processed for the detection of EC apoptosis, histology, and transmission electron microscopy (TEM) studies. There was a significantly higher number of TUNEL-positive ECs in infected compared to noninfected groups [0.2942 % (interquartile ranges (IR), 0.2941; n = 26) versus 0 % (IR, 0; n = 12), p < 0.01]. Caspase-3 staining was negative. Cholesterol diet together with infections induced widening of the subendothelial space and appearance of increased numbers of foam cells. TEM revealed degenerative changes in cytoplasmic organelles and signs of EC necrosis. In conclusion, infection leads to an increase in coronary EC death and seems to exacerbate cholesterol-induced intimal thickening and foam cell accumulation.}}, author = {{Birck, Malene M. and Saraste, Antti and Hyttel, Poul and Odermarsky, Michal and Liuba, Petru and Saukko, Pekka and Hansen, Axel K. and Pesonen, Erkki}}, issn = {{1937-5395}}, keywords = {{Atherosclerosis; Endothelial cells; Infection; Pig model; Cell death}}, language = {{eng}}, number = {{4}}, pages = {{579--587}}, publisher = {{Springer}}, series = {{Journal of Cardiovascular Translational Research}}, title = {{Endothelial Cell Death and Intimal Foam Cell Accumulation in the Coronary Artery of Infected Hypercholesterolemic Minipigs}}, url = {{http://dx.doi.org/10.1007/s12265-013-9463-2}}, doi = {{10.1007/s12265-013-9463-2}}, volume = {{6}}, year = {{2013}}, }