Direct role of FLT3 in regulation of early lymphoid progenitors
(2018) In British Journal of Haematology 183(4). p.588-600- Abstract
Given that FLT3 expression is highly restricted on lymphoid progenitors, it is possible that the established role of FLT3 in the regulation of B and T lymphopoiesis reflects its high expression and role in regulation of lymphoid-primed multipotent progenitors (LMPPs) or common lymphoid progenitors (CLPs). We generated a Flt3 conditional knock-out (Flt3fl/fl) mouse model to address the direct role of FLT3 in regulation of lymphoid-restricted progenitors, subsequent to turning on Rag1 expression, as well as potentially ontogeny-specific roles in B and T lymphopoiesis. Our studies establish a prominent and direct role of FLT3, independently of the established role of FLT3 in regulation of LMPPs and CLPs, in regulation of fetal... (More)
Given that FLT3 expression is highly restricted on lymphoid progenitors, it is possible that the established role of FLT3 in the regulation of B and T lymphopoiesis reflects its high expression and role in regulation of lymphoid-primed multipotent progenitors (LMPPs) or common lymphoid progenitors (CLPs). We generated a Flt3 conditional knock-out (Flt3fl/fl) mouse model to address the direct role of FLT3 in regulation of lymphoid-restricted progenitors, subsequent to turning on Rag1 expression, as well as potentially ontogeny-specific roles in B and T lymphopoiesis. Our studies establish a prominent and direct role of FLT3, independently of the established role of FLT3 in regulation of LMPPs and CLPs, in regulation of fetal as well as adult early B cell progenitors, and the early thymic progenitors (ETPs) in adult mice but not in the fetus. Our findings highlight the potential benefit of targeting poor prognosis acute B-cell progenitor leukaemia and ETP leukaemia with recurrent FLT3 mutations using clinical FLT3 inhibitors.
(Less)
- author
- Zriwil, Alya LU ; Böiers, Charlotta LU ; Kristiansen, Trine A. LU ; Wittmann, Lilian LU ; Yuan, Joan LU ; Nerlov, Claus LU ; Sitnicka, Ewa LU and Jacobsen, Sten E.W. LU
- organization
-
- Division of Molecular Hematology (DMH)
- Developmental lymphopoiesis and leukemia (research group)
- Division of Molecular Medicine and Gene Therapy
- Developmental Immunology (research group)
- Lymphoid Development and Regulation (research group)
- StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
- publishing date
- 2018-09-14
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- conditional knock-out mouse model, FLT3, haematopoiesis, lymphoid development, lymphoid progenitors
- in
- British Journal of Haematology
- volume
- 183
- issue
- 4
- pages
- 13 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:30596405
- scopus:85053465008
- ISSN
- 0007-1048
- DOI
- 10.1111/bjh.15578
- language
- English
- LU publication?
- yes
- id
- 40491f2b-de08-4186-9dd8-89db65d964dc
- date added to LUP
- 2018-10-23 12:37:08
- date last changed
- 2024-10-15 10:37:25
@article{40491f2b-de08-4186-9dd8-89db65d964dc, abstract = {{<p>Given that FLT3 expression is highly restricted on lymphoid progenitors, it is possible that the established role of FLT3 in the regulation of B and T lymphopoiesis reflects its high expression and role in regulation of lymphoid-primed multipotent progenitors (LMPPs) or common lymphoid progenitors (CLPs). We generated a Flt3 conditional knock-out (Flt3<sup>fl/fl</sup>) mouse model to address the direct role of FLT3 in regulation of lymphoid-restricted progenitors, subsequent to turning on Rag1 expression, as well as potentially ontogeny-specific roles in B and T lymphopoiesis. Our studies establish a prominent and direct role of FLT3, independently of the established role of FLT3 in regulation of LMPPs and CLPs, in regulation of fetal as well as adult early B cell progenitors, and the early thymic progenitors (ETPs) in adult mice but not in the fetus. Our findings highlight the potential benefit of targeting poor prognosis acute B-cell progenitor leukaemia and ETP leukaemia with recurrent FLT3 mutations using clinical FLT3 inhibitors.</p>}}, author = {{Zriwil, Alya and Böiers, Charlotta and Kristiansen, Trine A. and Wittmann, Lilian and Yuan, Joan and Nerlov, Claus and Sitnicka, Ewa and Jacobsen, Sten E.W.}}, issn = {{0007-1048}}, keywords = {{conditional knock-out mouse model; FLT3; haematopoiesis; lymphoid development; lymphoid progenitors}}, language = {{eng}}, month = {{09}}, number = {{4}}, pages = {{588--600}}, publisher = {{Wiley-Blackwell}}, series = {{British Journal of Haematology}}, title = {{Direct role of FLT3 in regulation of early lymphoid progenitors}}, url = {{http://dx.doi.org/10.1111/bjh.15578}}, doi = {{10.1111/bjh.15578}}, volume = {{183}}, year = {{2018}}, }