Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Sublethal in vitro glucose-oxygen deprivation protects cultured hippocampal neurons against a subsequent severe insult

Khaspekov, Leonid ; Shamloo, Mehrdad LU ; Victorov, Ilya and Wieloch, Tadeusz LU (1998) In NeuroReport 9(7). p.1273-1276
Abstract

Rat and gerbil hippocampus exposed to a sublethal period of ischemia becomes resistant to a subsequent period of lethal ischemia induced several days later, a phenomenon referred to as ischemic preconditioning. Here we describe ischemic preconditioning induced in vitro in cultured hippocampal neurons. Mixed neuroglial hippocampal cell cultures of 14-17 DIV were exposed to a combined glucose and oxygen deprivation (GOD). Cultures subjected to 90 min, but not 60 min, of GOD showed extensive degeneration after a 1 day recovery period. An episode of 60 min of preconditioning GOD followed 1 and 2 days later by 90 min of GOD resulted in 40-60% protection. The data demonstrate that ischemic preconditioning can be mimicked in an in vitro... (More)

Rat and gerbil hippocampus exposed to a sublethal period of ischemia becomes resistant to a subsequent period of lethal ischemia induced several days later, a phenomenon referred to as ischemic preconditioning. Here we describe ischemic preconditioning induced in vitro in cultured hippocampal neurons. Mixed neuroglial hippocampal cell cultures of 14-17 DIV were exposed to a combined glucose and oxygen deprivation (GOD). Cultures subjected to 90 min, but not 60 min, of GOD showed extensive degeneration after a 1 day recovery period. An episode of 60 min of preconditioning GOD followed 1 and 2 days later by 90 min of GOD resulted in 40-60% protection. The data demonstrate that ischemic preconditioning can be mimicked in an in vitro hippocampal cell culture system.

(Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Cell culture, Hippocampus, Ischemia, Neuronal death, Tolerance
in
NeuroReport
volume
9
issue
7
pages
1273 - 1276
publisher
Lippincott Williams & Wilkins
external identifiers
  • pmid:9631411
  • scopus:0032507599
ISSN
0959-4965
language
English
LU publication?
yes
id
4051f7d3-970a-4882-a022-f91876a23863
date added to LUP
2016-10-05 16:08:12
date last changed
2025-01-12 12:44:49
@article{4051f7d3-970a-4882-a022-f91876a23863,
  abstract     = {{<p>Rat and gerbil hippocampus exposed to a sublethal period of ischemia becomes resistant to a subsequent period of lethal ischemia induced several days later, a phenomenon referred to as ischemic preconditioning. Here we describe ischemic preconditioning induced in vitro in cultured hippocampal neurons. Mixed neuroglial hippocampal cell cultures of 14-17 DIV were exposed to a combined glucose and oxygen deprivation (GOD). Cultures subjected to 90 min, but not 60 min, of GOD showed extensive degeneration after a 1 day recovery period. An episode of 60 min of preconditioning GOD followed 1 and 2 days later by 90 min of GOD resulted in 40-60% protection. The data demonstrate that ischemic preconditioning can be mimicked in an in vitro hippocampal cell culture system.</p>}},
  author       = {{Khaspekov, Leonid and Shamloo, Mehrdad and Victorov, Ilya and Wieloch, Tadeusz}},
  issn         = {{0959-4965}},
  keywords     = {{Cell culture; Hippocampus; Ischemia; Neuronal death; Tolerance}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{7}},
  pages        = {{1273--1276}},
  publisher    = {{Lippincott Williams & Wilkins}},
  series       = {{NeuroReport}},
  title        = {{Sublethal in vitro glucose-oxygen deprivation protects cultured hippocampal neurons against a subsequent severe insult}},
  volume       = {{9}},
  year         = {{1998}},
}