Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Investigation of binding event perturbations caused by elevated QCM-D oscillation amplitude

Edvardsson, M ; Rodahl, M and Höök, Fredrik LU (2006) In Analyst 131(7). p.822-828
Abstract
We report measurements with the quartz crystal microbalance with dissipation monitoring (QCM-D) technique, with focus on how the shear oscillation amplitude of the sensor surface influences biorecognition binding events. Technically, this is made as reported recently ( M. Edvardsson, M. Rodahl, B. Kasemo, F. Hook, Anal. Chem., 2005, 77( 15), 4918-4926) by operating the QCM in dual frequency mode; one harmonic (n = n(1)) is utilized for continuous excitation of the QCM-D sensor at resonance at variable driving amplitudes (1-10 V), while the second harmonic (n not equal n(1)) is used for combined f and D measurements. By using one harmonic as a "probe" and the other one as an "actuator", elevated amplitudes can be used to perturb - or... (More)
We report measurements with the quartz crystal microbalance with dissipation monitoring (QCM-D) technique, with focus on how the shear oscillation amplitude of the sensor surface influences biorecognition binding events. Technically, this is made as reported recently ( M. Edvardsson, M. Rodahl, B. Kasemo, F. Hook, Anal. Chem., 2005, 77( 15), 4918-4926) by operating the QCM in dual frequency mode; one harmonic (n = n(1)) is utilized for continuous excitation of the QCM-D sensor at resonance at variable driving amplitudes (1-10 V), while the second harmonic (n not equal n(1)) is used for combined f and D measurements. By using one harmonic as a "probe" and the other one as an "actuator", elevated amplitudes can be used to perturb - or activate - binding reactions in a controlled way, while simultaneously maintaining the possibility of probing the adsorption and/or desorption events in a non-perturbative manner using combined f and D measurements. In this work we investigate the influence of oscillation amplitude variations on the binding of NeutrAvidin-modified polystyrene beads (O similar to 200 nm) to a planar biotin-modified lipid bilayer supported on an SiO2-modified QCM-D sensor. These results are further compared with data on an identical system, except that the NeutrAvidin-biotin recognition was replaced by fully complementary DNA hybridization. Supported by micrographs of the binding pattern, the results demonstrate that there exists, for both systems, a unique critical oscillation amplitude, A(c), below which binding is unaffected by the oscillation, and above which binding is efficiently prevented. Associated with A(c), there is a critical crystal radius, r(c), defining the central part of the crystal where binding is prevented. From QCM-D data, A(c) for the present system was estimated to be similar to 6.5 nm, yielding a value of r(c) of similar to 3 mm - the latter number was nicely confirmed by fluorescent- and dark-field micrographs of the crystal. Furthermore, the fact that A(c) is observed to be identical for the two types of biorecognition reactions suggests that it is neither the strength, nor the number of contact points, that determine the amplitude at which binding is prevented. Rather, particle size seems to be the determining parameter. (Less)
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analyst
volume
131
issue
7
pages
822 - 828
publisher
Royal Society of Chemistry
external identifiers
  • wos:000238560900009
  • scopus:33745496747
ISSN
1364-5528
DOI
10.1039/b601800a
language
English
LU publication?
yes
id
81621072-a6a5-4c8a-b514-3c8267cc6617 (old id 405784)
date added to LUP
2016-04-01 16:05:13
date last changed
2021-06-01 04:00:02
@article{81621072-a6a5-4c8a-b514-3c8267cc6617,
  abstract     = {We report measurements with the quartz crystal microbalance with dissipation monitoring (QCM-D) technique, with focus on how the shear oscillation amplitude of the sensor surface influences biorecognition binding events. Technically, this is made as reported recently ( M. Edvardsson, M. Rodahl, B. Kasemo, F. Hook, Anal. Chem., 2005, 77( 15), 4918-4926) by operating the QCM in dual frequency mode; one harmonic (n = n(1)) is utilized for continuous excitation of the QCM-D sensor at resonance at variable driving amplitudes (1-10 V), while the second harmonic (n not equal n(1)) is used for combined f and D measurements. By using one harmonic as a "probe" and the other one as an "actuator", elevated amplitudes can be used to perturb - or activate - binding reactions in a controlled way, while simultaneously maintaining the possibility of probing the adsorption and/or desorption events in a non-perturbative manner using combined f and D measurements. In this work we investigate the influence of oscillation amplitude variations on the binding of NeutrAvidin-modified polystyrene beads (O similar to 200 nm) to a planar biotin-modified lipid bilayer supported on an SiO2-modified QCM-D sensor. These results are further compared with data on an identical system, except that the NeutrAvidin-biotin recognition was replaced by fully complementary DNA hybridization. Supported by micrographs of the binding pattern, the results demonstrate that there exists, for both systems, a unique critical oscillation amplitude, A(c), below which binding is unaffected by the oscillation, and above which binding is efficiently prevented. Associated with A(c), there is a critical crystal radius, r(c), defining the central part of the crystal where binding is prevented. From QCM-D data, A(c) for the present system was estimated to be similar to 6.5 nm, yielding a value of r(c) of similar to 3 mm - the latter number was nicely confirmed by fluorescent- and dark-field micrographs of the crystal. Furthermore, the fact that A(c) is observed to be identical for the two types of biorecognition reactions suggests that it is neither the strength, nor the number of contact points, that determine the amplitude at which binding is prevented. Rather, particle size seems to be the determining parameter.},
  author       = {Edvardsson, M and Rodahl, M and Höök, Fredrik},
  issn         = {1364-5528},
  language     = {eng},
  number       = {7},
  pages        = {822--828},
  publisher    = {Royal Society of Chemistry},
  series       = {Analyst},
  title        = {Investigation of binding event perturbations caused by elevated QCM-D oscillation amplitude},
  url          = {http://dx.doi.org/10.1039/b601800a},
  doi          = {10.1039/b601800a},
  volume       = {131},
  year         = {2006},
}