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Aspects of estimating parameter dependencies in a detailed chromatography model based on frontal experiments

Persson, P ; Gustavsson, Per-Erik LU ; Zacchi, Guido LU and Nilsson, Bernt LU (2006) In Process Biochemistry 41(8). p.1812-1821
Abstract
A methodology for estimation of the dependency on flow rate and bead size of the axial dispersion coefficient and the film mass transfer coefficient in a detailed model for chromatographic processes is proposed. The model describes the concentration of the solute in the mobile phase and considers external/internal mass transfer resistance. The flow rate dependency of the mixing effect in the external volume is also considered. The unknown model parameters estimated are the bed void, the axial dispersion coefficient, the liquid film mass transfer coefficient, the effective diffusion coefficient and the apparent bead porosity. All the parameters in the model and the parameter dependencies were subsequently determined in three classes of... (More)
A methodology for estimation of the dependency on flow rate and bead size of the axial dispersion coefficient and the film mass transfer coefficient in a detailed model for chromatographic processes is proposed. The model describes the concentration of the solute in the mobile phase and considers external/internal mass transfer resistance. The flow rate dependency of the mixing effect in the external volume is also considered. The unknown model parameters estimated are the bed void, the axial dispersion coefficient, the liquid film mass transfer coefficient, the effective diffusion coefficient and the apparent bead porosity. All the parameters in the model and the parameter dependencies were subsequently determined in three classes of experiments, i.e. the external mixing behaviour, the mobile phase behaviour and the stationary phase behaviour. The estimates are based on the sum of the least squares of the residuals between the experimental breakthrough curves and the model response. The methodology is exemplified by frontal experiments with blue dextran and bovine serum albumin in a well-defined column set-up. The results show that it is possible to estimate the unknown physical parameters in the detailed model, and their dependencies on flow rate and bead size, using the proposed methodology. (c) 2006 Elsevier Ltd. All rights reserved. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
mass transfer resistance, computer simulation, chromatography modelling, parameter estimation, parameter dependencies
in
Process Biochemistry
volume
41
issue
8
pages
1812 - 1821
publisher
Elsevier
external identifiers
  • wos:000238398000015
  • scopus:33646802922
ISSN
1873-3298
DOI
10.1016/j.procbio.2006.03.030
language
English
LU publication?
yes
id
bf0018f6-de60-4b3a-a0ee-ea0c9c669f64 (old id 405850)
date added to LUP
2016-04-01 12:30:32
date last changed
2021-09-22 01:27:34
@article{bf0018f6-de60-4b3a-a0ee-ea0c9c669f64,
  abstract     = {A methodology for estimation of the dependency on flow rate and bead size of the axial dispersion coefficient and the film mass transfer coefficient in a detailed model for chromatographic processes is proposed. The model describes the concentration of the solute in the mobile phase and considers external/internal mass transfer resistance. The flow rate dependency of the mixing effect in the external volume is also considered. The unknown model parameters estimated are the bed void, the axial dispersion coefficient, the liquid film mass transfer coefficient, the effective diffusion coefficient and the apparent bead porosity. All the parameters in the model and the parameter dependencies were subsequently determined in three classes of experiments, i.e. the external mixing behaviour, the mobile phase behaviour and the stationary phase behaviour. The estimates are based on the sum of the least squares of the residuals between the experimental breakthrough curves and the model response. The methodology is exemplified by frontal experiments with blue dextran and bovine serum albumin in a well-defined column set-up. The results show that it is possible to estimate the unknown physical parameters in the detailed model, and their dependencies on flow rate and bead size, using the proposed methodology. (c) 2006 Elsevier Ltd. All rights reserved.},
  author       = {Persson, P and Gustavsson, Per-Erik and Zacchi, Guido and Nilsson, Bernt},
  issn         = {1873-3298},
  language     = {eng},
  number       = {8},
  pages        = {1812--1821},
  publisher    = {Elsevier},
  series       = {Process Biochemistry},
  title        = {Aspects of estimating parameter dependencies in a detailed chromatography model based on frontal experiments},
  url          = {http://dx.doi.org/10.1016/j.procbio.2006.03.030},
  doi          = {10.1016/j.procbio.2006.03.030},
  volume       = {41},
  year         = {2006},
}