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From gene expression analysis to tissue microarrays - A rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies

Ek, Sara LU ; Andreasson, Ulrika LU orcid ; Hober, S ; Kampf, C ; Ponten, F ; Uhlen, M ; Merz, H and Borrebaeck, Carl LU (2006) In Molecular & Cellular Proteomics 5(6). p.1072-1081
Abstract
Mantle cell lymphoma (MCL) is an aggressive lymphoid malignancy for which better treatment strategies are needed. To identify potential diagnostic and therapeutic targets, a signature consisting of MCL-associated genes was selected based on a comprehensive gene expression analysis of malignant and normal B cells. The corresponding protein epitope signature tags were identified and used to raise monospecific, polyclonal antibodies, which were subsequently analyzed on paraffin-embedded sections of malignant and normal tissue. In this study, we demonstrate that the initial selection strategy of MCL-associated genes successfully allows identification of protein antigens either uniquely expressed or overexpressed in MCL compared with normal... (More)
Mantle cell lymphoma (MCL) is an aggressive lymphoid malignancy for which better treatment strategies are needed. To identify potential diagnostic and therapeutic targets, a signature consisting of MCL-associated genes was selected based on a comprehensive gene expression analysis of malignant and normal B cells. The corresponding protein epitope signature tags were identified and used to raise monospecific, polyclonal antibodies, which were subsequently analyzed on paraffin-embedded sections of malignant and normal tissue. In this study, we demonstrate that the initial selection strategy of MCL-associated genes successfully allows identification of protein antigens either uniquely expressed or overexpressed in MCL compared with normal lymphoid tissues. We propose that genome-based, affinity proteomics, using protein epitope signature tag-induced antibodies, is an efficient way to rapidly identify a number of disease-associated protein candidates of both previously known and unknown identities. (Less)
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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular & Cellular Proteomics
volume
5
issue
6
pages
1072 - 1081
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • wos:000238288100009
  • pmid:16524965
  • scopus:33745610852
  • pmid:16524965
ISSN
1535-9484
DOI
10.1074/mcp.M600077-MCP200
language
English
LU publication?
yes
id
b0a6ef42-80ca-4484-83ea-ca5e25bb23f6 (old id 405937)
date added to LUP
2016-04-01 11:59:38
date last changed
2021-04-06 01:58:24
@article{b0a6ef42-80ca-4484-83ea-ca5e25bb23f6,
  abstract     = {Mantle cell lymphoma (MCL) is an aggressive lymphoid malignancy for which better treatment strategies are needed. To identify potential diagnostic and therapeutic targets, a signature consisting of MCL-associated genes was selected based on a comprehensive gene expression analysis of malignant and normal B cells. The corresponding protein epitope signature tags were identified and used to raise monospecific, polyclonal antibodies, which were subsequently analyzed on paraffin-embedded sections of malignant and normal tissue. In this study, we demonstrate that the initial selection strategy of MCL-associated genes successfully allows identification of protein antigens either uniquely expressed or overexpressed in MCL compared with normal lymphoid tissues. We propose that genome-based, affinity proteomics, using protein epitope signature tag-induced antibodies, is an efficient way to rapidly identify a number of disease-associated protein candidates of both previously known and unknown identities.},
  author       = {Ek, Sara and Andreasson, Ulrika and Hober, S and Kampf, C and Ponten, F and Uhlen, M and Merz, H and Borrebaeck, Carl},
  issn         = {1535-9484},
  language     = {eng},
  number       = {6},
  pages        = {1072--1081},
  publisher    = {American Society for Biochemistry and Molecular Biology},
  series       = {Molecular & Cellular Proteomics},
  title        = {From gene expression analysis to tissue microarrays - A rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies},
  url          = {http://dx.doi.org/10.1074/mcp.M600077-MCP200},
  doi          = {10.1074/mcp.M600077-MCP200},
  volume       = {5},
  year         = {2006},
}