From gene expression analysis to tissue microarrays - A rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies
Ek, Sara LU ; Andreasson, Ulrika LU
; Hober, S
; Kampf, C
; Ponten, F
; Uhlen, M
; Merz, H
and Borrebaeck, Carl
LU
(2006)
In Molecular & Cellular Proteomics
5(6).
p.1072-1081
- Abstract
- Mantle cell lymphoma (MCL) is an aggressive lymphoid malignancy for which better treatment strategies are needed. To identify potential diagnostic and therapeutic targets, a signature consisting of MCL-associated genes was selected based on a comprehensive gene expression analysis of malignant and normal B cells. The corresponding protein epitope signature tags were identified and used to raise monospecific, polyclonal antibodies, which were subsequently analyzed on paraffin-embedded sections of malignant and normal tissue. In this study, we demonstrate that the initial selection strategy of MCL-associated genes successfully allows identification of protein antigens either uniquely expressed or overexpressed in MCL compared with normal... (More)
- Mantle cell lymphoma (MCL) is an aggressive lymphoid malignancy for which better treatment strategies are needed. To identify potential diagnostic and therapeutic targets, a signature consisting of MCL-associated genes was selected based on a comprehensive gene expression analysis of malignant and normal B cells. The corresponding protein epitope signature tags were identified and used to raise monospecific, polyclonal antibodies, which were subsequently analyzed on paraffin-embedded sections of malignant and normal tissue. In this study, we demonstrate that the initial selection strategy of MCL-associated genes successfully allows identification of protein antigens either uniquely expressed or overexpressed in MCL compared with normal lymphoid tissues. We propose that genome-based, affinity proteomics, using protein epitope signature tag-induced antibodies, is an efficient way to rapidly identify a number of disease-associated protein candidates of both previously known and unknown identities. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/405937
- author
- Ek, Sara
LU
; Andreasson, Ulrika
LU
; Hober, S
; Kampf, C
; Ponten, F
; Uhlen, M
; Merz, H
and Borrebaeck, Carl
LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular & Cellular Proteomics
- volume
- 5
- issue
- 6
- pages
- 1072 - 1081
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- wos:000238288100009
- pmid:16524965
- scopus:33745610852
- pmid:16524965
- ISSN
- 1535-9484
- DOI
- 10.1074/mcp.M600077-MCP200
- language
- English
- LU publication?
- yes
- id
- b0a6ef42-80ca-4484-83ea-ca5e25bb23f6 (old id 405937)
- date added to LUP
- 2016-04-01 11:59:38
- date last changed
- 2022-02-03 08:03:04
@article{b0a6ef42-80ca-4484-83ea-ca5e25bb23f6,
abstract = {{Mantle cell lymphoma (MCL) is an aggressive lymphoid malignancy for which better treatment strategies are needed. To identify potential diagnostic and therapeutic targets, a signature consisting of MCL-associated genes was selected based on a comprehensive gene expression analysis of malignant and normal B cells. The corresponding protein epitope signature tags were identified and used to raise monospecific, polyclonal antibodies, which were subsequently analyzed on paraffin-embedded sections of malignant and normal tissue. In this study, we demonstrate that the initial selection strategy of MCL-associated genes successfully allows identification of protein antigens either uniquely expressed or overexpressed in MCL compared with normal lymphoid tissues. We propose that genome-based, affinity proteomics, using protein epitope signature tag-induced antibodies, is an efficient way to rapidly identify a number of disease-associated protein candidates of both previously known and unknown identities.}},
author = {{Ek, Sara and Andreasson, Ulrika and Hober, S and Kampf, C and Ponten, F and Uhlen, M and Merz, H and Borrebaeck, Carl}},
issn = {{1535-9484}},
language = {{eng}},
number = {{6}},
pages = {{1072--1081}},
publisher = {{American Society for Biochemistry and Molecular Biology}},
series = {{Molecular & Cellular Proteomics}},
title = {{From gene expression analysis to tissue microarrays - A rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies}},
url = {{http://dx.doi.org/10.1074/mcp.M600077-MCP200}},
doi = {{10.1074/mcp.M600077-MCP200}},
volume = {{5}},
year = {{2006}},
}