Rapid induction of apoptosis in B-cell lymphoma by functionally isolated human antibodies
(2006) In International Journal of Cancer 119(2). p.349-358- Abstract
- Novel panning and screening methodology was devised to isolate high affinity human recombinant scFv antibody fragments with functionally associated properties in B lymphoma cells. The approach was used to generate a panel of apoptosis-inducing antibodies specific for antigens differentially expressed in B lymphoma vs. T leukaemia cells. The selections resulted in an antibody pool with near perfect selectivity (> 99%) for the B lymphoma target cells. Randomly picked clones (72) revealed 7 unique antibody genotypes. Six of these rapidly induced apoptosis in target cells. Following the conversion to full IgGs, the antibodies were shown to be specific for HLA-DR/DP, the B-cell receptor p chain and for CD54/ICAM-1. The latter receptor was... (More)
- Novel panning and screening methodology was devised to isolate high affinity human recombinant scFv antibody fragments with functionally associated properties in B lymphoma cells. The approach was used to generate a panel of apoptosis-inducing antibodies specific for antigens differentially expressed in B lymphoma vs. T leukaemia cells. The selections resulted in an antibody pool with near perfect selectivity (> 99%) for the B lymphoma target cells. Randomly picked clones (72) revealed 7 unique antibody genotypes. Six of these rapidly induced apoptosis in target cells. Following the conversion to full IgGs, the antibodies were shown to be specific for HLA-DR/DP, the B-cell receptor p chain and for CD54/ICAM-1. The latter receptor was not previously associated with apoptotic properties in B-cell lymphomas. Anti-ICAM-1 IgG induced apoptosis in a broad range of B lymphoma cell lines and were shown by immunohistochemistry to bind strongly to B lymphoma tissue obtained from 5 different B lymphoma patients. The recombinant IgG antibodies had affinities in the subnanomolar (0.3 nM) to nanomolar (3 nM) range. The described technology is generally applicable for the rapid isolation of high affinity human antibodies with specificity for differentially expressed cell surface receptors with intrinsic negative or positive signalling properties from naive phage libraries. (c) 2006 Wiley-Liss, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/406062
- author
- Fransson, Johan LU ; Tornberg, UC ; Borrebaeck, Carl LU ; Carlsson, Roland LU and Frendeus, B
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- intercellular, apoptosis, antibody library, phage display, B lymphoma, adhesion molecule-1
- in
- International Journal of Cancer
- volume
- 119
- issue
- 2
- pages
- 349 - 358
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000238267300014
- pmid:16477633
- scopus:33745220111
- ISSN
- 0020-7136
- DOI
- 10.1002/ijc.21829
- language
- English
- LU publication?
- yes
- id
- 0a41254e-efcf-4e97-bad1-f3950d689454 (old id 406062)
- date added to LUP
- 2016-04-01 12:31:51
- date last changed
- 2022-01-27 06:21:56
@article{0a41254e-efcf-4e97-bad1-f3950d689454,
abstract = {{Novel panning and screening methodology was devised to isolate high affinity human recombinant scFv antibody fragments with functionally associated properties in B lymphoma cells. The approach was used to generate a panel of apoptosis-inducing antibodies specific for antigens differentially expressed in B lymphoma vs. T leukaemia cells. The selections resulted in an antibody pool with near perfect selectivity (> 99%) for the B lymphoma target cells. Randomly picked clones (72) revealed 7 unique antibody genotypes. Six of these rapidly induced apoptosis in target cells. Following the conversion to full IgGs, the antibodies were shown to be specific for HLA-DR/DP, the B-cell receptor p chain and for CD54/ICAM-1. The latter receptor was not previously associated with apoptotic properties in B-cell lymphomas. Anti-ICAM-1 IgG induced apoptosis in a broad range of B lymphoma cell lines and were shown by immunohistochemistry to bind strongly to B lymphoma tissue obtained from 5 different B lymphoma patients. The recombinant IgG antibodies had affinities in the subnanomolar (0.3 nM) to nanomolar (3 nM) range. The described technology is generally applicable for the rapid isolation of high affinity human antibodies with specificity for differentially expressed cell surface receptors with intrinsic negative or positive signalling properties from naive phage libraries. (c) 2006 Wiley-Liss, Inc.}},
author = {{Fransson, Johan and Tornberg, UC and Borrebaeck, Carl and Carlsson, Roland and Frendeus, B}},
issn = {{0020-7136}},
keywords = {{intercellular; apoptosis; antibody library; phage display; B lymphoma; adhesion molecule-1}},
language = {{eng}},
number = {{2}},
pages = {{349--358}},
publisher = {{John Wiley & Sons Inc.}},
series = {{International Journal of Cancer}},
title = {{Rapid induction of apoptosis in B-cell lymphoma by functionally isolated human antibodies}},
url = {{http://dx.doi.org/10.1002/ijc.21829}},
doi = {{10.1002/ijc.21829}},
volume = {{119}},
year = {{2006}},
}