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Characterization of semenogelin proteins in the human retina

Bonilha, Vera L. ; Rayborn, Mary E. ; Shadrach, Karen ; Lundwall, Åke LU ; Malm, Johan LU ; Bhattacharya, Sanjoy K. ; Crabb, John W. and Hollyfield, Joe G. (2006) In Experimental Eye Research 83(1). p.120-127
Abstract
Semenogelin I and II are the major proteins present in semen coagulum. In the present study, semenogelin I and II were detected in human RPE lysates by proteomic analysis. We further analyzed the expression of these proteins in the retinal cells in vivo and in vitro. Western blots detected semenogelin I and II in both RPE and neural retina while the vitreous contained only SgII. Cryo and paraffin sections of human retina were processed for both immunofluorescence and DAB reaction with an antibody that recognizes both forms of semenogelin proteins. Retina and RPE total lysates were evaluated for the presence of these proteins and in a human RPE cell line (D407). Both proteins were detected by western blot in human RPE and in D407 cell... (More)
Semenogelin I and II are the major proteins present in semen coagulum. In the present study, semenogelin I and II were detected in human RPE lysates by proteomic analysis. We further analyzed the expression of these proteins in the retinal cells in vivo and in vitro. Western blots detected semenogelin I and II in both RPE and neural retina while the vitreous contained only SgII. Cryo and paraffin sections of human retina were processed for both immunofluorescence and DAB reaction with an antibody that recognizes both forms of semenogelin proteins. Retina and RPE total lysates were evaluated for the presence of these proteins and in a human RPE cell line (D407). Both proteins were detected by western blot in human RPE and in D407 cell lysates. Immunoreactivity was detected in the ganglion cell and photoreceptor layer of the retina. Our data support the expression of semenogelin I and II in the human retina in several different compartments. Further studies towards addressing the function of these proteins in the retina are in progress. (c) 2006 Elsevier Ltd. All rights reserved. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
proteomics, RPE, retina, semenogelins, immunohistochemistry
in
Experimental Eye Research
volume
83
issue
1
pages
120 - 127
publisher
Elsevier
external identifiers
  • wos:000238408400013
  • pmid:16545373
  • scopus:33646870139
  • pmid:16545373
ISSN
0014-4835
DOI
10.1016/j.exer.2005.11.011
language
English
LU publication?
yes
id
b7a16ac5-c6da-45a7-8716-9416b429a0df (old id 406109)
date added to LUP
2016-04-01 11:47:25
date last changed
2020-01-12 08:36:50
@article{b7a16ac5-c6da-45a7-8716-9416b429a0df,
  abstract     = {Semenogelin I and II are the major proteins present in semen coagulum. In the present study, semenogelin I and II were detected in human RPE lysates by proteomic analysis. We further analyzed the expression of these proteins in the retinal cells in vivo and in vitro. Western blots detected semenogelin I and II in both RPE and neural retina while the vitreous contained only SgII. Cryo and paraffin sections of human retina were processed for both immunofluorescence and DAB reaction with an antibody that recognizes both forms of semenogelin proteins. Retina and RPE total lysates were evaluated for the presence of these proteins and in a human RPE cell line (D407). Both proteins were detected by western blot in human RPE and in D407 cell lysates. Immunoreactivity was detected in the ganglion cell and photoreceptor layer of the retina. Our data support the expression of semenogelin I and II in the human retina in several different compartments. Further studies towards addressing the function of these proteins in the retina are in progress. (c) 2006 Elsevier Ltd. All rights reserved.},
  author       = {Bonilha, Vera L. and Rayborn, Mary E. and Shadrach, Karen and Lundwall, Åke and Malm, Johan and Bhattacharya, Sanjoy K. and Crabb, John W. and Hollyfield, Joe G.},
  issn         = {0014-4835},
  language     = {eng},
  number       = {1},
  pages        = {120--127},
  publisher    = {Elsevier},
  series       = {Experimental Eye Research},
  title        = {Characterization of semenogelin proteins in the human retina},
  url          = {http://dx.doi.org/10.1016/j.exer.2005.11.011},
  doi          = {10.1016/j.exer.2005.11.011},
  volume       = {83},
  year         = {2006},
}