Urinary Apolipoprotein M Could Be Used as a Biomarker of Acute Renal Injury: An Ischemia-Reperfusion Injury Model of Kidney in Rat
(2013) In Transplantation Proceedings 45(6). p.2476-2479- Abstract
- Background. It has been well documented that apolipoprotein M (apoM) is principally expressed in hepatocytes as well as renal tubular epithelial cells. The importance of apoM in the kidney is unknown. In the present study we examined urinary any apoM after short-term ischemia-reperfusion injury (IRI) of kidney in a rat model. Methods. The kidneys of 11 male Sprague-Dawley rats were rendered ischemic for 45 minutes followed by different intervals of reperfusion. Serum and urine apoM concentrations were determined using a dot-blot analysis with specific rabbit anti-human apoM antibodies that cross-react with rat apoM. Serum concentrations of blood urea nitrogen (BUN) and creatinine (Cr) were determined using standard clinical automated... (More)
- Background. It has been well documented that apolipoprotein M (apoM) is principally expressed in hepatocytes as well as renal tubular epithelial cells. The importance of apoM in the kidney is unknown. In the present study we examined urinary any apoM after short-term ischemia-reperfusion injury (IRI) of kidney in a rat model. Methods. The kidneys of 11 male Sprague-Dawley rats were rendered ischemic for 45 minutes followed by different intervals of reperfusion. Serum and urine apoM concentrations were determined using a dot-blot analysis with specific rabbit anti-human apoM antibodies that cross-react with rat apoM. Serum concentrations of blood urea nitrogen (BUN) and creatinine (Cr) were determined using standard clinical automated analyses. Results. BUN was significantly elevated after 45 minutes of ischemia followed by 24 hours of reperfusion; serum Cr concentrations were also significantly increased at 6 and 24 hours of reperfusion. Interestingly, similar to BUN and Cr, serum apoM concentrations were significantly increased after ischemia for 45 minutes alone and after 2 hours of reperfusion. Urinary apoM concentrations were obviously increased after 2 h as well as 6 hours of reperfusion. Conclusion. apoM showed characteristics of an acute-phase reactive protein; its occurrence in urine may be considered to be a biomarker of acute renal injury. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4062560
- author
- Xu, X. -L. ; Mao, Q. -Y. ; Luo, G. -H. ; Nilsson-Ehle, Peter LU ; He, X. -Z. and Xu, Ning LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Transplantation Proceedings
- volume
- 45
- issue
- 6
- pages
- 2476 - 2479
- publisher
- Elsevier
- external identifiers
-
- wos:000323852000071
- scopus:84882259224
- pmid:23953565
- ISSN
- 0041-1345
- DOI
- 10.1016/j.transproceed.2013.04.009
- language
- English
- LU publication?
- yes
- id
- 3884e1ec-a8c5-47f4-a606-60a1c8245195 (old id 4062560)
- date added to LUP
- 2016-04-01 10:20:37
- date last changed
- 2022-04-04 17:06:33
@article{3884e1ec-a8c5-47f4-a606-60a1c8245195, abstract = {{Background. It has been well documented that apolipoprotein M (apoM) is principally expressed in hepatocytes as well as renal tubular epithelial cells. The importance of apoM in the kidney is unknown. In the present study we examined urinary any apoM after short-term ischemia-reperfusion injury (IRI) of kidney in a rat model. Methods. The kidneys of 11 male Sprague-Dawley rats were rendered ischemic for 45 minutes followed by different intervals of reperfusion. Serum and urine apoM concentrations were determined using a dot-blot analysis with specific rabbit anti-human apoM antibodies that cross-react with rat apoM. Serum concentrations of blood urea nitrogen (BUN) and creatinine (Cr) were determined using standard clinical automated analyses. Results. BUN was significantly elevated after 45 minutes of ischemia followed by 24 hours of reperfusion; serum Cr concentrations were also significantly increased at 6 and 24 hours of reperfusion. Interestingly, similar to BUN and Cr, serum apoM concentrations were significantly increased after ischemia for 45 minutes alone and after 2 hours of reperfusion. Urinary apoM concentrations were obviously increased after 2 h as well as 6 hours of reperfusion. Conclusion. apoM showed characteristics of an acute-phase reactive protein; its occurrence in urine may be considered to be a biomarker of acute renal injury.}}, author = {{Xu, X. -L. and Mao, Q. -Y. and Luo, G. -H. and Nilsson-Ehle, Peter and He, X. -Z. and Xu, Ning}}, issn = {{0041-1345}}, language = {{eng}}, number = {{6}}, pages = {{2476--2479}}, publisher = {{Elsevier}}, series = {{Transplantation Proceedings}}, title = {{Urinary Apolipoprotein M Could Be Used as a Biomarker of Acute Renal Injury: An Ischemia-Reperfusion Injury Model of Kidney in Rat}}, url = {{http://dx.doi.org/10.1016/j.transproceed.2013.04.009}}, doi = {{10.1016/j.transproceed.2013.04.009}}, volume = {{45}}, year = {{2013}}, }