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Histamine and chromogranin A-derived peptides in rat stomach ECL cells

Norlén, Per LU (2000)
Abstract
The ECL cells constitute the predominant endocrine cell population of the stomach. They secrete histamine which is a major stimulator of acid secretion. Chormogranin A (CgA)-derived peptides, such as pancreastatin, are co-secreted with histamine. In this thesis it was demonstrated that pancreastatin may serve as a reliable marker for ECL cell secretory activity in the rat. The function of pancreastatin and other CgA-derived peptides is however obscure, and therefore, attempts were made to develop a method for continuous monitoring of ECL-cell histamine in conscious rats. By using microdialysis it was demonstrated that histamine is secreted from ECL cells in response to gastrin and food and to a number of neuropeptides, such as PACAP, VIP... (More)
The ECL cells constitute the predominant endocrine cell population of the stomach. They secrete histamine which is a major stimulator of acid secretion. Chormogranin A (CgA)-derived peptides, such as pancreastatin, are co-secreted with histamine. In this thesis it was demonstrated that pancreastatin may serve as a reliable marker for ECL cell secretory activity in the rat. The function of pancreastatin and other CgA-derived peptides is however obscure, and therefore, attempts were made to develop a method for continuous monitoring of ECL-cell histamine in conscious rats. By using microdialysis it was demonstrated that histamine is secreted from ECL cells in response to gastrin and food and to a number of neuropeptides, such as PACAP, VIP and adrenaline. It could also be demonstrated that anaesthesia greatly impairs ECL-cell histamine secretion. (Less)
Abstract (Swedish)
Popular Abstract in Swedish

ECL-cellen är en hormonproducerande cell i magslemhinnan. Den utsöndrar histamin vilken i sin tur reglerar magsäckens saltsyrautsöndring. I avhandlingen utvecklades en metod som möjliggör mätningar av histaminutsöndringen från ECL-celler i vakna råttor. Metoden bygger på att ett semipermeabelt membran opereras in i magslemhinnan varpå histamin och andra substanser som finns i vävnaden kan diffundera över membranet och analyseras.
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author
supervisor
opponent
  • Prof. Waldum, Helge L., Trondheim, Norge
organization
publishing date
type
Thesis
publication status
published
subject
keywords
pharmacy, pharmacognosy, Pharmacological sciences, CGRP adrenaline, galanin, VIP, PACAP, somatostatin, gastrin, microdialys, pancreastatin, chromogranin A, histamine, ECL cell, rat stomach, toxicology, Farmakologi, farmakognosi, farmaci, toxikologi, Endocrinology, secreting systems, diabetology, Endokrinologi, sekretion, diabetologi
pages
187 pages
publisher
Per Norlén, Dept. of Pharmacol. Sölveg. 10, S-22362 Lund, Sweden.,
defense location
Lecture Hall, Dept. of Pharmacology
defense date
2000-09-29 09:15:00
external identifiers
  • other:ISRN: LUMEDW/MEFA--1041--SE
language
English
LU publication?
yes
id
1c4d7581-4dde-4894-927d-8b8ac1cbe390 (old id 40737)
date added to LUP
2016-04-04 10:34:42
date last changed
2018-11-21 20:59:34
@phdthesis{1c4d7581-4dde-4894-927d-8b8ac1cbe390,
  abstract     = {The ECL cells constitute the predominant endocrine cell population of the stomach. They secrete histamine which is a major stimulator of acid secretion. Chormogranin A (CgA)-derived peptides, such as pancreastatin, are co-secreted with histamine. In this thesis it was demonstrated that pancreastatin may serve as a reliable marker for ECL cell secretory activity in the rat. The function of pancreastatin and other CgA-derived peptides is however obscure, and therefore, attempts were made to develop a method for continuous monitoring of ECL-cell histamine in conscious rats. By using microdialysis it was demonstrated that histamine is secreted from ECL cells in response to gastrin and food and to a number of neuropeptides, such as PACAP, VIP and adrenaline. It could also be demonstrated that anaesthesia greatly impairs ECL-cell histamine secretion.},
  author       = {Norlén, Per},
  language     = {eng},
  publisher    = {Per Norlén, Dept. of Pharmacol. Sölveg. 10, S-22362 Lund, Sweden.,},
  school       = {Lund University},
  title        = {Histamine and chromogranin A-derived peptides in rat stomach ECL cells},
  year         = {2000},
}