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New directions in non-porous membrane extraction

Thordarson, Eddie LU (2000)
Abstract (Swedish)
Popular Abstract in Swedish

Avhandlingen berör membranbaserade tekniker för provupparbetning, vilket enkelt uttryckt innebär att använda membran för att ”skilja agnarna från vetet”. De membran som använts kan designas för att vara selektivt genomsläppliga, d.vs. släppa igenom vissa molekyler och inte andra. Denna selektivitet behöver inte vara baserad på molekylstorlek, tvärtom styrs den mer av molekylernas laddning och ”vattenskräck”. För att generalisera kan man säga att ämnen är antingen mer lösliga i vatten eller i organiska ämnen. Hur som helst, separerar membranet intressanta föreningar ifrån de föreningar som annars skulle försvåra eller omöjliggöra analysen. Viktigt här är också att membranet tillåter anrikning av... (More)
Popular Abstract in Swedish

Avhandlingen berör membranbaserade tekniker för provupparbetning, vilket enkelt uttryckt innebär att använda membran för att ”skilja agnarna från vetet”. De membran som använts kan designas för att vara selektivt genomsläppliga, d.vs. släppa igenom vissa molekyler och inte andra. Denna selektivitet behöver inte vara baserad på molekylstorlek, tvärtom styrs den mer av molekylernas laddning och ”vattenskräck”. För att generalisera kan man säga att ämnen är antingen mer lösliga i vatten eller i organiska ämnen. Hur som helst, separerar membranet intressanta föreningar ifrån de föreningar som annars skulle försvåra eller omöjliggöra analysen. Viktigt här är också att membranet tillåter anrikning av de intressanta föreningarna, d.v.s. halten av ämnena är högre i slutprovet än i ursprungsprovet. En annan central del har varit att koppla dessa membran till instrument som separerar provkomponenterna ifrån varandra och slutligen mäter halterna. Ett genomgående spår i arbetet har varit miniatyrisering och den problematik som omger denna. Fördelar med att miniatyrisera är bl.a. ökad hanterbarhet av små provvolymer (ex. blodprover ur barn) samt minskad användning av dyra och hälsofarliga organiska lösningsmedel (Less)
Abstract
Complex biological and environmental samples require a high degree of selectivity in order to determine components therein. Sensitivity measures, such as analyte pre-concentration are often required for assessing composition quantitatively. Use of membranes and especially non-porous membranes for extraction purposes offers in many cases the desired measure of selectivity and sensitivity for trace analysis from such samples. Selected species can be either permanently charged, ionogenic or neutral, and range in size from protons to proteins. The result of the extractions is often excellent cleanup of the matrix and a decrease in detection limits. Another salient feature with these systems is that they offer an extremely low consumption of... (More)
Complex biological and environmental samples require a high degree of selectivity in order to determine components therein. Sensitivity measures, such as analyte pre-concentration are often required for assessing composition quantitatively. Use of membranes and especially non-porous membranes for extraction purposes offers in many cases the desired measure of selectivity and sensitivity for trace analysis from such samples. Selected species can be either permanently charged, ionogenic or neutral, and range in size from protons to proteins. The result of the extractions is often excellent cleanup of the matrix and a decrease in detection limits. Another salient feature with these systems is that they offer an extremely low consumption of potentially hazardous, organic solvents. The work in this thesis has focused on novel approaches within the field of non-porous membrane extraction, including system integration and automation, miniaturisation and extensions of technical scope and applicability. Integration has been designed to suit a number of important techniques, such as normal-phase and reversed-phase liquid chromatography (LC), capillary-LC, gas chromatography (GC), capillary electrophoresis (CE) and continuous-flow systems (FIA/SIA). The development of a miniaturised membrane unit, carrying a single hollow fibre, was devised and integrated with capillary-LC and with CE. An easy connection between membrane based liquid-liquid extraction and GC has been proposed. Extending the applicability of non-porous membrane extractions, antibodies have been used for selective recognition and trapping. (Less)
Please use this url to cite or link to this publication:
author
opponent
  • Dr Majors, Ron E., Hewlett Packard, Delaware, USA
organization
publishing date
type
Thesis
publication status
published
subject
keywords
extracting syringe, solvent-free, miniaturisation, micro-porous membrane liquid-liquid extraction, polymeric membrane extraction, Non-porous membrane extraction, supported liquid extraction, integration, automation, Analytical chemistry, Analytisk kemi
pages
120 pages
publisher
Department of Analytical Chemistry, Lund University
defense location
Hörsal A at the Chemical Center
defense date
2000-10-13 13:13
external identifiers
  • other:ISRN: LUNKDL/(NKAK-1055)/(2000)
language
English
LU publication?
yes
id
5b349c8a-bfd4-4924-9702-fdf6c490d01a (old id 40800)
date added to LUP
2007-10-14 17:09:09
date last changed
2016-09-19 08:45:15
@phdthesis{5b349c8a-bfd4-4924-9702-fdf6c490d01a,
  abstract     = {Complex biological and environmental samples require a high degree of selectivity in order to determine components therein. Sensitivity measures, such as analyte pre-concentration are often required for assessing composition quantitatively. Use of membranes and especially non-porous membranes for extraction purposes offers in many cases the desired measure of selectivity and sensitivity for trace analysis from such samples. Selected species can be either permanently charged, ionogenic or neutral, and range in size from protons to proteins. The result of the extractions is often excellent cleanup of the matrix and a decrease in detection limits. Another salient feature with these systems is that they offer an extremely low consumption of potentially hazardous, organic solvents. The work in this thesis has focused on novel approaches within the field of non-porous membrane extraction, including system integration and automation, miniaturisation and extensions of technical scope and applicability. Integration has been designed to suit a number of important techniques, such as normal-phase and reversed-phase liquid chromatography (LC), capillary-LC, gas chromatography (GC), capillary electrophoresis (CE) and continuous-flow systems (FIA/SIA). The development of a miniaturised membrane unit, carrying a single hollow fibre, was devised and integrated with capillary-LC and with CE. An easy connection between membrane based liquid-liquid extraction and GC has been proposed. Extending the applicability of non-porous membrane extractions, antibodies have been used for selective recognition and trapping.},
  author       = {Thordarson, Eddie},
  keyword      = {extracting syringe,solvent-free,miniaturisation,micro-porous membrane liquid-liquid extraction,polymeric membrane extraction,Non-porous membrane extraction,supported liquid extraction,integration,automation,Analytical chemistry,Analytisk kemi},
  language     = {eng},
  pages        = {120},
  publisher    = {Department of Analytical Chemistry, Lund University},
  school       = {Lund University},
  title        = {New directions in non-porous membrane extraction},
  year         = {2000},
}