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Direct capture of plasmid DNA from non-clarified bacterial lysate using polycation-grafted monoliths

Hanora, Amro LU ; Savina, Irina LU ; Plieva, FM ; Izumrudov, VA ; Mattiasson, Bo LU and Galaev, Igor LU (2006) In Journal of Biotechnology 123(3). p.343-355
Abstract
Monolith columns from macroporous polyacrylamide gel were grafted with polycations, poly(N,N-dimethylaminoethyl methacrylate) (polyDMAEMA), (2-(methacryloyloxy)ethyl)-trimethyl ammonium chloride (polyMETA) and partially quaternized polyDMAEMA prepared via treating polyDMAEMA-grafted columns with propylbromide. The polymer grafting degrees varied between 34 and 110%. The polycation-grafted monolithic columns are able to capture plasmid DNA directly from alkaline lysate of Escherichia coli cells. Due to the large pore size in macroporous monoliths the particulate material present in nonclarified feeds did not block the columns. The captured plasmid DNA was eluted with I M NaCl as particulate-free preparation with significantly reduced... (More)
Monolith columns from macroporous polyacrylamide gel were grafted with polycations, poly(N,N-dimethylaminoethyl methacrylate) (polyDMAEMA), (2-(methacryloyloxy)ethyl)-trimethyl ammonium chloride (polyMETA) and partially quaternized polyDMAEMA prepared via treating polyDMAEMA-grafted columns with propylbromide. The polymer grafting degrees varied between 34 and 110%. The polycation-grafted monolithic columns are able to capture plasmid DNA directly from alkaline lysate of Escherichia coli cells. Due to the large pore size in macroporous monoliths the particulate material present in nonclarified feeds did not block the columns. The captured plasmid DNA was eluted with I M NaCl as particulate-free preparation with significantly reduced content of protein and RNA as compared to the applied lysate. (c) 2005 Elsevier B.V. All rights reserved. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
tentacle, plasmid DNA, supermacroporous gel, monolith column, chromatography
in
Journal of Biotechnology
volume
123
issue
3
pages
343 - 355
publisher
Elsevier
external identifiers
  • wos:000237881900009
  • pmid:16406156
  • scopus:33646481927
ISSN
1873-4863
DOI
10.1016/j.jbiotec.2005.11.017
language
English
LU publication?
yes
id
dcae062f-68ac-4499-9e4c-b13fdb64e0ad (old id 408346)
date added to LUP
2016-04-01 12:25:16
date last changed
2021-06-16 05:57:24
@article{dcae062f-68ac-4499-9e4c-b13fdb64e0ad,
  abstract     = {Monolith columns from macroporous polyacrylamide gel were grafted with polycations, poly(N,N-dimethylaminoethyl methacrylate) (polyDMAEMA), (2-(methacryloyloxy)ethyl)-trimethyl ammonium chloride (polyMETA) and partially quaternized polyDMAEMA prepared via treating polyDMAEMA-grafted columns with propylbromide. The polymer grafting degrees varied between 34 and 110%. The polycation-grafted monolithic columns are able to capture plasmid DNA directly from alkaline lysate of Escherichia coli cells. Due to the large pore size in macroporous monoliths the particulate material present in nonclarified feeds did not block the columns. The captured plasmid DNA was eluted with I M NaCl as particulate-free preparation with significantly reduced content of protein and RNA as compared to the applied lysate. (c) 2005 Elsevier B.V. All rights reserved.},
  author       = {Hanora, Amro and Savina, Irina and Plieva, FM and Izumrudov, VA and Mattiasson, Bo and Galaev, Igor},
  issn         = {1873-4863},
  language     = {eng},
  number       = {3},
  pages        = {343--355},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {Direct capture of plasmid DNA from non-clarified bacterial lysate using polycation-grafted monoliths},
  url          = {http://dx.doi.org/10.1016/j.jbiotec.2005.11.017},
  doi          = {10.1016/j.jbiotec.2005.11.017},
  volume       = {123},
  year         = {2006},
}