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The cervical lymph node preparation: A novel approach to study lymphocyte homing by intravital microscopy

Schramm, R ; Schafers, HJ ; Harder, Y ; Schmits, R ; Thorlacius, Henrik LU and Menger, MD (2006) In Inflammation Research 55(4). p.160-167
Abstract
Objective: Lymphocyte recirculation constitutes an integral part of the adaptive immune system. Blood-borne lymphocytes migrate into secondary lymphoid organs, crossing the vascular wall of site-specific high endothelial venules (HEVs). We created a preparation of the cervical lymph node in mice to study lymphocyte homing in vivo. Methods and Results: Our novel approach allowed the detailed analysis of hemodynamics and lymphocyte-HEV endothelium interactions by means of intravital fluorescence microscopy. We confirm the key roles of L-selectin and LFA-1 for lymphocyte homing. Blockade of L-selectin function inhibited lymphocyte rolling and firm adhesion by 92 % and 66%. In LFA-1-deficient mice, lymphocyte firm adhesion was reduced by 70%.... (More)
Objective: Lymphocyte recirculation constitutes an integral part of the adaptive immune system. Blood-borne lymphocytes migrate into secondary lymphoid organs, crossing the vascular wall of site-specific high endothelial venules (HEVs). We created a preparation of the cervical lymph node in mice to study lymphocyte homing in vivo. Methods and Results: Our novel approach allowed the detailed analysis of hemodynamics and lymphocyte-HEV endothelium interactions by means of intravital fluorescence microscopy. We confirm the key roles of L-selectin and LFA-1 for lymphocyte homing. Blockade of L-selectin function inhibited lymphocyte rolling and firm adhesion by 92 % and 66%. In LFA-1-deficient mice, lymphocyte firm adhesion was reduced by 70%. In addition to the microcirculation studies, the cervical lymph node preparation allowed for visualization of afferent lymphatic transport, which is mainly derived from the oral mucosa. Conclusion: This study reports a novel technical tool for the detailed in vivo analysis of adaptive immune responses. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
recirculation, adhesion, homing, lymphocytes
in
Inflammation Research
volume
55
issue
4
pages
160 - 167
publisher
Birkhäuser Verlag
external identifiers
  • wos:000237699400005
  • scopus:33745601959
ISSN
1420-908X
DOI
10.1007/s00011-006-0066-0
language
English
LU publication?
yes
id
ad75b0ef-d1c8-445e-8c2e-dffc9d2b5fd6 (old id 408636)
date added to LUP
2016-04-01 11:36:30
date last changed
2020-01-12 08:14:55
@article{ad75b0ef-d1c8-445e-8c2e-dffc9d2b5fd6,
  abstract     = {Objective: Lymphocyte recirculation constitutes an integral part of the adaptive immune system. Blood-borne lymphocytes migrate into secondary lymphoid organs, crossing the vascular wall of site-specific high endothelial venules (HEVs). We created a preparation of the cervical lymph node in mice to study lymphocyte homing in vivo. Methods and Results: Our novel approach allowed the detailed analysis of hemodynamics and lymphocyte-HEV endothelium interactions by means of intravital fluorescence microscopy. We confirm the key roles of L-selectin and LFA-1 for lymphocyte homing. Blockade of L-selectin function inhibited lymphocyte rolling and firm adhesion by 92 % and 66%. In LFA-1-deficient mice, lymphocyte firm adhesion was reduced by 70%. In addition to the microcirculation studies, the cervical lymph node preparation allowed for visualization of afferent lymphatic transport, which is mainly derived from the oral mucosa. Conclusion: This study reports a novel technical tool for the detailed in vivo analysis of adaptive immune responses.},
  author       = {Schramm, R and Schafers, HJ and Harder, Y and Schmits, R and Thorlacius, Henrik and Menger, MD},
  issn         = {1420-908X},
  language     = {eng},
  number       = {4},
  pages        = {160--167},
  publisher    = {Birkhäuser Verlag},
  series       = {Inflammation Research},
  title        = {The cervical lymph node preparation: A novel approach to study lymphocyte homing by intravital microscopy},
  url          = {http://dx.doi.org/10.1007/s00011-006-0066-0},
  doi          = {10.1007/s00011-006-0066-0},
  volume       = {55},
  year         = {2006},
}