The cervical lymph node preparation: A novel approach to study lymphocyte homing by intravital microscopy
(2006) In Inflammation Research 55(4). p.160-167- Abstract
- Objective: Lymphocyte recirculation constitutes an integral part of the adaptive immune system. Blood-borne lymphocytes migrate into secondary lymphoid organs, crossing the vascular wall of site-specific high endothelial venules (HEVs). We created a preparation of the cervical lymph node in mice to study lymphocyte homing in vivo. Methods and Results: Our novel approach allowed the detailed analysis of hemodynamics and lymphocyte-HEV endothelium interactions by means of intravital fluorescence microscopy. We confirm the key roles of L-selectin and LFA-1 for lymphocyte homing. Blockade of L-selectin function inhibited lymphocyte rolling and firm adhesion by 92 % and 66%. In LFA-1-deficient mice, lymphocyte firm adhesion was reduced by 70%.... (More)
- Objective: Lymphocyte recirculation constitutes an integral part of the adaptive immune system. Blood-borne lymphocytes migrate into secondary lymphoid organs, crossing the vascular wall of site-specific high endothelial venules (HEVs). We created a preparation of the cervical lymph node in mice to study lymphocyte homing in vivo. Methods and Results: Our novel approach allowed the detailed analysis of hemodynamics and lymphocyte-HEV endothelium interactions by means of intravital fluorescence microscopy. We confirm the key roles of L-selectin and LFA-1 for lymphocyte homing. Blockade of L-selectin function inhibited lymphocyte rolling and firm adhesion by 92 % and 66%. In LFA-1-deficient mice, lymphocyte firm adhesion was reduced by 70%. In addition to the microcirculation studies, the cervical lymph node preparation allowed for visualization of afferent lymphatic transport, which is mainly derived from the oral mucosa. Conclusion: This study reports a novel technical tool for the detailed in vivo analysis of adaptive immune responses. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/408636
- author
- Schramm, R ; Schafers, HJ ; Harder, Y ; Schmits, R ; Thorlacius, Henrik LU and Menger, MD
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- recirculation, adhesion, homing, lymphocytes
- in
- Inflammation Research
- volume
- 55
- issue
- 4
- pages
- 160 - 167
- publisher
- Birkhäuser Verlag
- external identifiers
-
- wos:000237699400005
- scopus:33745601959
- ISSN
- 1420-908X
- DOI
- 10.1007/s00011-006-0066-0
- language
- English
- LU publication?
- yes
- id
- ad75b0ef-d1c8-445e-8c2e-dffc9d2b5fd6 (old id 408636)
- date added to LUP
- 2016-04-01 11:36:30
- date last changed
- 2022-01-26 07:31:59
@article{ad75b0ef-d1c8-445e-8c2e-dffc9d2b5fd6,
abstract = {{Objective: Lymphocyte recirculation constitutes an integral part of the adaptive immune system. Blood-borne lymphocytes migrate into secondary lymphoid organs, crossing the vascular wall of site-specific high endothelial venules (HEVs). We created a preparation of the cervical lymph node in mice to study lymphocyte homing in vivo. Methods and Results: Our novel approach allowed the detailed analysis of hemodynamics and lymphocyte-HEV endothelium interactions by means of intravital fluorescence microscopy. We confirm the key roles of L-selectin and LFA-1 for lymphocyte homing. Blockade of L-selectin function inhibited lymphocyte rolling and firm adhesion by 92 % and 66%. In LFA-1-deficient mice, lymphocyte firm adhesion was reduced by 70%. In addition to the microcirculation studies, the cervical lymph node preparation allowed for visualization of afferent lymphatic transport, which is mainly derived from the oral mucosa. Conclusion: This study reports a novel technical tool for the detailed in vivo analysis of adaptive immune responses.}},
author = {{Schramm, R and Schafers, HJ and Harder, Y and Schmits, R and Thorlacius, Henrik and Menger, MD}},
issn = {{1420-908X}},
keywords = {{recirculation; adhesion; homing; lymphocytes}},
language = {{eng}},
number = {{4}},
pages = {{160--167}},
publisher = {{Birkhäuser Verlag}},
series = {{Inflammation Research}},
title = {{The cervical lymph node preparation: A novel approach to study lymphocyte homing by intravital microscopy}},
url = {{http://dx.doi.org/10.1007/s00011-006-0066-0}},
doi = {{10.1007/s00011-006-0066-0}},
volume = {{55}},
year = {{2006}},
}