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Direct heterogeneous electron transfer reactions of fungal laccases at bare and thiol-modified gold electrodes

Pita, M ; Shleev, Sergey LU ; Ruzgas, T ; Fernandez, VM ; Yaropolov, AI and Gorton, Lo LU (2006) In Electrochemistry Communications 8(5). p.747-753
Abstract
Mediatorless (direct) electron transfer between bare and thiol-modified gold electrodes and fungal laccases from different sources has been demonstrated. The electrochemical activity of the enzymes from basidiomycetes Trametes hirstita. Trametes ochracea, and Cerrena maxima under aerobic and anaerobic conditions can clearly be observed using cyclic voltammetry and spectroelectrochemistry. Bioelectroreduction of oxygen by T. hirsuta laccase immobilized on amino-thiophenol-modified gold electrodes., starting at +625 mV vs. NHE, is demonstrated and differences in bioelectrocatalysis of the enzyme immobilized on bare and thiol-modified electrodes are shown. It was found that hydrogen peroxide was one of the products of oxygen electroreduction... (More)
Mediatorless (direct) electron transfer between bare and thiol-modified gold electrodes and fungal laccases from different sources has been demonstrated. The electrochemical activity of the enzymes from basidiomycetes Trametes hirstita. Trametes ochracea, and Cerrena maxima under aerobic and anaerobic conditions can clearly be observed using cyclic voltammetry and spectroelectrochemistry. Bioelectroreduction of oxygen by T. hirsuta laccase immobilized on amino-thiophenol-modified gold electrodes., starting at +625 mV vs. NHE, is demonstrated and differences in bioelectrocatalysis of the enzyme immobilized on bare and thiol-modified electrodes are shown. It was found that hydrogen peroxide was one of the products of oxygen electroreduction on gold electrodes modified with fungal laccases, whereas no significant peroxide formation was observed for T. hirsuta laccase immobilised on thiol-modified gold electrodes. Thus, a hypothesis about two different mechanisms of oxygen electroreduction by fungal laccases adsorbed on bare and thiol-modified electrodes is proposed. (c) 2006 Elsevier B.V. All rights reserved. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cyclic, voltammetry, gold electrode, and T3 sites, T2, T1, laccase, redox potential
in
Electrochemistry Communications
volume
8
issue
5
pages
747 - 753
publisher
Elsevier
external identifiers
  • wos:000237776300013
  • scopus:33646190025
ISSN
1388-2481
DOI
10.1016/j.elecom.2006.03.008
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
id
fa75c9f8-5d16-4c83-9d59-aaec4a0f3b71 (old id 408681)
date added to LUP
2016-04-01 11:42:18
date last changed
2021-08-25 02:35:01
@article{fa75c9f8-5d16-4c83-9d59-aaec4a0f3b71,
  abstract     = {Mediatorless (direct) electron transfer between bare and thiol-modified gold electrodes and fungal laccases from different sources has been demonstrated. The electrochemical activity of the enzymes from basidiomycetes Trametes hirstita. Trametes ochracea, and Cerrena maxima under aerobic and anaerobic conditions can clearly be observed using cyclic voltammetry and spectroelectrochemistry. Bioelectroreduction of oxygen by T. hirsuta laccase immobilized on amino-thiophenol-modified gold electrodes., starting at +625 mV vs. NHE, is demonstrated and differences in bioelectrocatalysis of the enzyme immobilized on bare and thiol-modified electrodes are shown. It was found that hydrogen peroxide was one of the products of oxygen electroreduction on gold electrodes modified with fungal laccases, whereas no significant peroxide formation was observed for T. hirsuta laccase immobilised on thiol-modified gold electrodes. Thus, a hypothesis about two different mechanisms of oxygen electroreduction by fungal laccases adsorbed on bare and thiol-modified electrodes is proposed. (c) 2006 Elsevier B.V. All rights reserved.},
  author       = {Pita, M and Shleev, Sergey and Ruzgas, T and Fernandez, VM and Yaropolov, AI and Gorton, Lo},
  issn         = {1388-2481},
  language     = {eng},
  number       = {5},
  pages        = {747--753},
  publisher    = {Elsevier},
  series       = {Electrochemistry Communications},
  title        = {Direct heterogeneous electron transfer reactions of fungal laccases at bare and thiol-modified gold electrodes},
  url          = {http://dx.doi.org/10.1016/j.elecom.2006.03.008},
  doi          = {10.1016/j.elecom.2006.03.008},
  volume       = {8},
  year         = {2006},
}