Direct heterogeneous electron transfer reactions of fungal laccases at bare and thiol-modified gold electrodes
(2006) In Electrochemistry Communications 8(5). p.747-753- Abstract
- Mediatorless (direct) electron transfer between bare and thiol-modified gold electrodes and fungal laccases from different sources has been demonstrated. The electrochemical activity of the enzymes from basidiomycetes Trametes hirstita. Trametes ochracea, and Cerrena maxima under aerobic and anaerobic conditions can clearly be observed using cyclic voltammetry and spectroelectrochemistry. Bioelectroreduction of oxygen by T. hirsuta laccase immobilized on amino-thiophenol-modified gold electrodes., starting at +625 mV vs. NHE, is demonstrated and differences in bioelectrocatalysis of the enzyme immobilized on bare and thiol-modified electrodes are shown. It was found that hydrogen peroxide was one of the products of oxygen electroreduction... (More)
- Mediatorless (direct) electron transfer between bare and thiol-modified gold electrodes and fungal laccases from different sources has been demonstrated. The electrochemical activity of the enzymes from basidiomycetes Trametes hirstita. Trametes ochracea, and Cerrena maxima under aerobic and anaerobic conditions can clearly be observed using cyclic voltammetry and spectroelectrochemistry. Bioelectroreduction of oxygen by T. hirsuta laccase immobilized on amino-thiophenol-modified gold electrodes., starting at +625 mV vs. NHE, is demonstrated and differences in bioelectrocatalysis of the enzyme immobilized on bare and thiol-modified electrodes are shown. It was found that hydrogen peroxide was one of the products of oxygen electroreduction on gold electrodes modified with fungal laccases, whereas no significant peroxide formation was observed for T. hirsuta laccase immobilised on thiol-modified gold electrodes. Thus, a hypothesis about two different mechanisms of oxygen electroreduction by fungal laccases adsorbed on bare and thiol-modified electrodes is proposed. (c) 2006 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/408681
- author
- Pita, M ; Shleev, Sergey LU ; Ruzgas, T ; Fernandez, VM ; Yaropolov, AI and Gorton, Lo LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cyclic, voltammetry, gold electrode, and T3 sites, T2, T1, laccase, redox potential
- in
- Electrochemistry Communications
- volume
- 8
- issue
- 5
- pages
- 747 - 753
- publisher
- Elsevier
- external identifiers
-
- wos:000237776300013
- scopus:33646190025
- ISSN
- 1388-2481
- DOI
- 10.1016/j.elecom.2006.03.008
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- fa75c9f8-5d16-4c83-9d59-aaec4a0f3b71 (old id 408681)
- date added to LUP
- 2016-04-01 11:42:18
- date last changed
- 2022-02-18 03:40:16
@article{fa75c9f8-5d16-4c83-9d59-aaec4a0f3b71, abstract = {{Mediatorless (direct) electron transfer between bare and thiol-modified gold electrodes and fungal laccases from different sources has been demonstrated. The electrochemical activity of the enzymes from basidiomycetes Trametes hirstita. Trametes ochracea, and Cerrena maxima under aerobic and anaerobic conditions can clearly be observed using cyclic voltammetry and spectroelectrochemistry. Bioelectroreduction of oxygen by T. hirsuta laccase immobilized on amino-thiophenol-modified gold electrodes., starting at +625 mV vs. NHE, is demonstrated and differences in bioelectrocatalysis of the enzyme immobilized on bare and thiol-modified electrodes are shown. It was found that hydrogen peroxide was one of the products of oxygen electroreduction on gold electrodes modified with fungal laccases, whereas no significant peroxide formation was observed for T. hirsuta laccase immobilised on thiol-modified gold electrodes. Thus, a hypothesis about two different mechanisms of oxygen electroreduction by fungal laccases adsorbed on bare and thiol-modified electrodes is proposed. (c) 2006 Elsevier B.V. All rights reserved.}}, author = {{Pita, M and Shleev, Sergey and Ruzgas, T and Fernandez, VM and Yaropolov, AI and Gorton, Lo}}, issn = {{1388-2481}}, keywords = {{cyclic; voltammetry; gold electrode; and T3 sites; T2; T1; laccase; redox potential}}, language = {{eng}}, number = {{5}}, pages = {{747--753}}, publisher = {{Elsevier}}, series = {{Electrochemistry Communications}}, title = {{Direct heterogeneous electron transfer reactions of fungal laccases at bare and thiol-modified gold electrodes}}, url = {{http://dx.doi.org/10.1016/j.elecom.2006.03.008}}, doi = {{10.1016/j.elecom.2006.03.008}}, volume = {{8}}, year = {{2006}}, }