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Production of functional protein hydrolysates from Egyptian breeds of soybean and lupin seeds

Khalil, AA ; Mohamed, SS ; Taha, FS and Nordberg Karlsson, Eva LU orcid (2006) In African Journal of Biotechnology 5(10). p.907-916
Abstract
Enzymatic hydrolysis is an agro-processing aid that can be utilized in order to improve nutritional quality of protein extracts from many sources. In this study, protein extracts from ungerminated and/or germinated local Egyptian soybean and lupin flours were hydrolyzed using the enzyme papain. The hydrolysis processes were carried out for 2 h and aliquots were withdrawn at different time intervals. We have analysed the protein hydrolysate after 30 min hydrolysis as an example of a partially hydrolyzed protein, and after 120 min as an example of greatly hydrolyzed protein. The hydrolysate (2 h treatment at 80 degrees C and pH 7.4) from both soybean and lupin flour contained significantly decreased trypsin inhibitor activity and urease... (More)
Enzymatic hydrolysis is an agro-processing aid that can be utilized in order to improve nutritional quality of protein extracts from many sources. In this study, protein extracts from ungerminated and/or germinated local Egyptian soybean and lupin flours were hydrolyzed using the enzyme papain. The hydrolysis processes were carried out for 2 h and aliquots were withdrawn at different time intervals. We have analysed the protein hydrolysate after 30 min hydrolysis as an example of a partially hydrolyzed protein, and after 120 min as an example of greatly hydrolyzed protein. The hydrolysate (2 h treatment at 80 degrees C and pH 7.4) from both soybean and lupin flour contained significantly decreased trypsin inhibitor activity and urease activity, and a reduced phytate content, which improved the overall protein quality. Hydrolysis caused almost complete inactivation of urease in all soybean and lupin samples regardless if the seeds were germinated or not. High protein content, nitrogen solubility and in vitro protein digestibility was shown after hydrolysis. Total protein content (in g/100 g extract) increased in hydrolyzed samples from 48.1 to 51-60 for soybean (dependent on pre-treatment) and from 36.8 to 39.9-48.6 for lupin. Total essential amino acid content was also increased in papain hydrolyzed samples, compared to that in raw and germinated legumes. More specifically, the amount of lysine, sulphur amino acids, histidine, and to a certain extent isoleucine and threonine increased in samples from both legume species. All soybean samples exhibited antioxidant activity while in lupin samples, only those subjected to hydrolysis showed activity. Generally, it was clearly observed that the longer the duration of enzymatic hydrolysis (within the time frame of the experiment), the higher the improvement of the nutritional quality. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
extract, protein, papain, enzymatic hydrolysis, germination, lupin, soybean, nutritive value
in
African Journal of Biotechnology
volume
5
issue
10
pages
907 - 916
publisher
Academic Journals
external identifiers
  • wos:000237675000025
  • scopus:33646904868
ISSN
1684-5315
language
English
LU publication?
yes
id
f9a6a023-1c4d-4d47-bc53-9d95f4b4996b (old id 408774)
alternative location
http://www.academicjournals.org/AJB/abstracts/abs2006/16May/Khalil%20et%20al.htm
date added to LUP
2016-04-01 16:52:32
date last changed
2020-10-20 03:52:51
@article{f9a6a023-1c4d-4d47-bc53-9d95f4b4996b,
  abstract     = {Enzymatic hydrolysis is an agro-processing aid that can be utilized in order to improve nutritional quality of protein extracts from many sources. In this study, protein extracts from ungerminated and/or germinated local Egyptian soybean and lupin flours were hydrolyzed using the enzyme papain. The hydrolysis processes were carried out for 2 h and aliquots were withdrawn at different time intervals. We have analysed the protein hydrolysate after 30 min hydrolysis as an example of a partially hydrolyzed protein, and after 120 min as an example of greatly hydrolyzed protein. The hydrolysate (2 h treatment at 80 degrees C and pH 7.4) from both soybean and lupin flour contained significantly decreased trypsin inhibitor activity and urease activity, and a reduced phytate content, which improved the overall protein quality. Hydrolysis caused almost complete inactivation of urease in all soybean and lupin samples regardless if the seeds were germinated or not. High protein content, nitrogen solubility and in vitro protein digestibility was shown after hydrolysis. Total protein content (in g/100 g extract) increased in hydrolyzed samples from 48.1 to 51-60 for soybean (dependent on pre-treatment) and from 36.8 to 39.9-48.6 for lupin. Total essential amino acid content was also increased in papain hydrolyzed samples, compared to that in raw and germinated legumes. More specifically, the amount of lysine, sulphur amino acids, histidine, and to a certain extent isoleucine and threonine increased in samples from both legume species. All soybean samples exhibited antioxidant activity while in lupin samples, only those subjected to hydrolysis showed activity. Generally, it was clearly observed that the longer the duration of enzymatic hydrolysis (within the time frame of the experiment), the higher the improvement of the nutritional quality.},
  author       = {Khalil, AA and Mohamed, SS and Taha, FS and Nordberg Karlsson, Eva},
  issn         = {1684-5315},
  language     = {eng},
  number       = {10},
  pages        = {907--916},
  publisher    = {Academic Journals},
  series       = {African Journal of Biotechnology},
  title        = {Production of functional protein hydrolysates from Egyptian breeds of soybean and lupin seeds},
  url          = {http://www.academicjournals.org/AJB/abstracts/abs2006/16May/Khalil%20et%20al.htm},
  volume       = {5},
  year         = {2006},
}