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The transcription factor KLF11 can induce gamma-globin gene expression in the setting of in vivo adult erythropoiesis

Emery, D. W.; Gavriilidis, Georgios LU ; Asano, H. and Stamatoyannopoulos, G. (2007) In Journal of Cellular Biochemistry 100(4). p.55-1045
Abstract
Previous studies in a fetal erythroid cell line demonstrated that the transcription factor, Kruppel-like factor 11 (KLF11), could specifically induce transcription from a gamma-globin gene promoter, and that this induction was mediated through a specific canonical CACCC cis-DNA binding motif. We report here that ectopic expression of KLF11 can also induce fetal gamma-globin gene expression in the setting of adult erythropoiesis both in vitro and in vivo. Studies in an adult-stage murine erythroleukemia (MEL) cell line demonstrated that retrovirus vector-mediated transduction of KLF11 could increase both the amount of expression from a basally active, but not from a overtly silenced, recombinant gamma-globin transgene, as well as the... (More)
Previous studies in a fetal erythroid cell line demonstrated that the transcription factor, Kruppel-like factor 11 (KLF11), could specifically induce transcription from a gamma-globin gene promoter, and that this induction was mediated through a specific canonical CACCC cis-DNA binding motif. We report here that ectopic expression of KLF11 can also induce fetal gamma-globin gene expression in the setting of adult erythropoiesis both in vitro and in vivo. Studies in an adult-stage murine erythroleukemia (MEL) cell line demonstrated that retrovirus vector-mediated transduction of KLF11 could increase both the amount of expression from a basally active, but not from a overtly silenced, recombinant gamma-globin transgene, as well as the frequency of cells expressing this transgene. A similar pattern of gamma-globin gene induction was also observed both in vitro and in vivo following KLF11 transduction of bone marrow from mice containing a basally active gamma-globin transgene. These studies provide the first evidence that ectopic expression of a transcription factor can induce gamma-globin gene expression in vivo during adult erythropoiesis. (Less)
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author
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published
subject
keywords
Kruppel-Like Transcription Factors/genetics/metabolism/*physiology, Humans, Green Fluorescent Proteins/genetics/metabolism, Globins/*genetics/metabolism, *Gene Expression, Flow Cytometry, Erythropoiesis/*genetics, Erythroid Precursor Cells/cytology/metabolism, Cell Line, Animals, Bone Marrow Transplantation, Mice, Transgenic, NIH 3T3 Cells, Retroviridae/genetics, Time Factors, Transfection
in
Journal of Cellular Biochemistry
volume
100
issue
4
pages
55 - 1045
publisher
Wiley-Blackwell
external identifiers
  • scopus:33847039607
ISSN
0730-2312
DOI
10.1002/jcb.21093
language
English
LU publication?
no
id
c7058b32-66c5-40f3-a7a3-b9f54a7c05aa (old id 4091043)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/17131378
http://www.ncbi.nlm.nih.gov/pubmed/17131378
date added to LUP
2013-10-10 09:39:20
date last changed
2017-01-01 07:36:58
@article{c7058b32-66c5-40f3-a7a3-b9f54a7c05aa,
  abstract     = {Previous studies in a fetal erythroid cell line demonstrated that the transcription factor, Kruppel-like factor 11 (KLF11), could specifically induce transcription from a gamma-globin gene promoter, and that this induction was mediated through a specific canonical CACCC cis-DNA binding motif. We report here that ectopic expression of KLF11 can also induce fetal gamma-globin gene expression in the setting of adult erythropoiesis both in vitro and in vivo. Studies in an adult-stage murine erythroleukemia (MEL) cell line demonstrated that retrovirus vector-mediated transduction of KLF11 could increase both the amount of expression from a basally active, but not from a overtly silenced, recombinant gamma-globin transgene, as well as the frequency of cells expressing this transgene. A similar pattern of gamma-globin gene induction was also observed both in vitro and in vivo following KLF11 transduction of bone marrow from mice containing a basally active gamma-globin transgene. These studies provide the first evidence that ectopic expression of a transcription factor can induce gamma-globin gene expression in vivo during adult erythropoiesis.},
  author       = {Emery, D. W. and Gavriilidis, Georgios and Asano, H. and Stamatoyannopoulos, G.},
  issn         = {0730-2312},
  keyword      = {Kruppel-Like Transcription Factors/genetics/metabolism/*physiology,Humans,Green Fluorescent Proteins/genetics/metabolism,Globins/*genetics/metabolism,*Gene Expression,Flow Cytometry,Erythropoiesis/*genetics,Erythroid Precursor Cells/cytology/metabolism,Cell Line,Animals,Bone Marrow Transplantation,Mice,Transgenic,NIH 3T3 Cells,Retroviridae/genetics,Time Factors,Transfection},
  language     = {eng},
  number       = {4},
  pages        = {55--1045},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Cellular Biochemistry},
  title        = {The transcription factor KLF11 can induce gamma-globin gene expression in the setting of in vivo adult erythropoiesis},
  url          = {http://dx.doi.org/10.1002/jcb.21093},
  volume       = {100},
  year         = {2007},
}