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Functional study of transcription factor KLF11 by targeted gene inactivation

Song, C. Z.; Gavriilidis, Georgios LU ; Asano, H. and Stamatoyannopoulos, G. (2005) In Blood Cells, Molecules & Diseases 34(1). p.53-59
Abstract
Sp1/Kruppel-like factor (KLF) family of transcription factors regulates diverse biological processes including cell growth, differentiation, and development through modulation of gene expression. This family of factors regulates transcription positively and negatively by binding to the GC and GT/CACCC boxes in the promoter through their highly conserved three zinc finger domains. Although the molecular mechanism of gene regulation by this family of proteins has been well studied, their exact role in growth and development in vivo remains largely unknown. KLF11 has been implicated in the regulation of cell growth and gene expression. To determine the physiological function of KLF11, we generated KLF11-null mice by gene-targeting technology.... (More)
Sp1/Kruppel-like factor (KLF) family of transcription factors regulates diverse biological processes including cell growth, differentiation, and development through modulation of gene expression. This family of factors regulates transcription positively and negatively by binding to the GC and GT/CACCC boxes in the promoter through their highly conserved three zinc finger domains. Although the molecular mechanism of gene regulation by this family of proteins has been well studied, their exact role in growth and development in vivo remains largely unknown. KLF11 has been implicated in the regulation of cell growth and gene expression. To determine the physiological function of KLF11, we generated KLF11-null mice by gene-targeting technology. Homologous KLF11(-/-) mice were bred normally and were fertile. Hematopoiesis at all stages of development was normal in the KLF11(-/-) mice. There was no effect on globin gene expression. These mice lived as long as the wild-type mice without evident pathological defects. Thus, despite its cell growth inhibition and transcriptional regulation functions observed when transiently or stably expressed in cultured cells in vitro, the results from genetic knockout suggest that KLF11 is not absolutely required for hematopoiesis, growth, and development. (Less)
Please use this url to cite or link to this publication:
author
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Contribution to journal
publication status
published
subject
keywords
Knockout, Mice, Globins/genetics, Gene Expression Regulation, DNA-Binding Proteins/*deficiency/genetics/*metabolism, Body Weight/genetics, Animals, Blood Cell Count, Survival Rate, Transcription Factors/*deficiency/genetics/*metabolism
in
Blood Cells, Molecules & Diseases
volume
34
issue
1
pages
53 - 59
publisher
Elsevier
external identifiers
  • scopus:10944224983
ISSN
1096-0961
DOI
10.1016/j.bcmd.2004.08.027
language
English
LU publication?
no
id
65c07c6e-a95a-4c9d-9d5d-e2679cb41c0c (old id 4091051)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/15607700
date added to LUP
2016-01-25 14:09:48
date last changed
2017-08-20 04:36:57
@article{65c07c6e-a95a-4c9d-9d5d-e2679cb41c0c,
  abstract     = {Sp1/Kruppel-like factor (KLF) family of transcription factors regulates diverse biological processes including cell growth, differentiation, and development through modulation of gene expression. This family of factors regulates transcription positively and negatively by binding to the GC and GT/CACCC boxes in the promoter through their highly conserved three zinc finger domains. Although the molecular mechanism of gene regulation by this family of proteins has been well studied, their exact role in growth and development in vivo remains largely unknown. KLF11 has been implicated in the regulation of cell growth and gene expression. To determine the physiological function of KLF11, we generated KLF11-null mice by gene-targeting technology. Homologous KLF11(-/-) mice were bred normally and were fertile. Hematopoiesis at all stages of development was normal in the KLF11(-/-) mice. There was no effect on globin gene expression. These mice lived as long as the wild-type mice without evident pathological defects. Thus, despite its cell growth inhibition and transcriptional regulation functions observed when transiently or stably expressed in cultured cells in vitro, the results from genetic knockout suggest that KLF11 is not absolutely required for hematopoiesis, growth, and development.},
  author       = {Song, C. Z. and Gavriilidis, Georgios and Asano, H. and Stamatoyannopoulos, G.},
  issn         = {1096-0961},
  keyword      = {Knockout,Mice,Globins/genetics,Gene Expression Regulation,DNA-Binding Proteins/*deficiency/genetics/*metabolism,Body Weight/genetics,Animals,Blood Cell Count,Survival Rate,Transcription Factors/*deficiency/genetics/*metabolism},
  language     = {eng},
  number       = {1},
  pages        = {53--59},
  publisher    = {Elsevier},
  series       = {Blood Cells, Molecules & Diseases},
  title        = {Functional study of transcription factor KLF11 by targeted gene inactivation},
  url          = {http://dx.doi.org/10.1016/j.bcmd.2004.08.027},
  volume       = {34},
  year         = {2005},
}