Self-assembly formation of multiple DNA-tethered lipid bilayers
(2009) In Journal of Structural Biology 168(1). p.6-200- Abstract
Inspired by natural cell-cell junctions, where membrane-residing proteins control the separation between two or more membranes without interfering with their integrity, we report a new self-assembly route for formation of multiple highly fluid tethered lipid bilayers with the inter-membrane volume geometrically confined by membrane-anchored DNA duplexes. The formation of multiple planar membrane-membrane junctions were accomplished using disk shaped bicelles, composed of a mixture of the long-chained dimyristoyl phosphatidylcholine (DMPC) and the short-chained dihexanoyl PC further stabilized with the positively charged detergent hexadecyl-trimethyl-ammonium bromide (CTAB). Quartz crystal microbalance with dissipation (QCM-D) monitoring... (More)
Inspired by natural cell-cell junctions, where membrane-residing proteins control the separation between two or more membranes without interfering with their integrity, we report a new self-assembly route for formation of multiple highly fluid tethered lipid bilayers with the inter-membrane volume geometrically confined by membrane-anchored DNA duplexes. The formation of multiple planar membrane-membrane junctions were accomplished using disk shaped bicelles, composed of a mixture of the long-chained dimyristoyl phosphatidylcholine (DMPC) and the short-chained dihexanoyl PC further stabilized with the positively charged detergent hexadecyl-trimethyl-ammonium bromide (CTAB). Quartz crystal microbalance with dissipation (QCM-D) monitoring and fluorescence microscopy and fluorescence recovery after photobleaching (FRAP) were used to monitor the formation and to characterize the integrity of the self-assembled lipid-DNA architecture.
(Less)
- author
- Tabaei, Seyed R ; Jönsson, Peter LU ; Brändén, Magnus and Höök, Fredrik LU
- publishing date
- 2009-10
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- DNA, Dimyristoylphosphatidylcholine, Lipid Bilayers, Models, Theoretical, Journal Article, Research Support, Non-U.S. Gov't
- in
- Journal of Structural Biology
- volume
- 168
- issue
- 1
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:69249216546
- pmid:19607925
- ISSN
- 1095-8657
- DOI
- 10.1016/j.jsb.2009.07.008
- language
- English
- LU publication?
- no
- id
- 40915abb-7145-47e5-99d2-460405833b9f
- date added to LUP
- 2018-01-26 10:51:17
- date last changed
- 2024-03-01 13:12:44
@article{40915abb-7145-47e5-99d2-460405833b9f,
abstract = {{<p>Inspired by natural cell-cell junctions, where membrane-residing proteins control the separation between two or more membranes without interfering with their integrity, we report a new self-assembly route for formation of multiple highly fluid tethered lipid bilayers with the inter-membrane volume geometrically confined by membrane-anchored DNA duplexes. The formation of multiple planar membrane-membrane junctions were accomplished using disk shaped bicelles, composed of a mixture of the long-chained dimyristoyl phosphatidylcholine (DMPC) and the short-chained dihexanoyl PC further stabilized with the positively charged detergent hexadecyl-trimethyl-ammonium bromide (CTAB). Quartz crystal microbalance with dissipation (QCM-D) monitoring and fluorescence microscopy and fluorescence recovery after photobleaching (FRAP) were used to monitor the formation and to characterize the integrity of the self-assembled lipid-DNA architecture.</p>}},
author = {{Tabaei, Seyed R and Jönsson, Peter and Brändén, Magnus and Höök, Fredrik}},
issn = {{1095-8657}},
keywords = {{DNA; Dimyristoylphosphatidylcholine; Lipid Bilayers; Models, Theoretical; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
number = {{1}},
pages = {{6--200}},
publisher = {{Elsevier}},
series = {{Journal of Structural Biology}},
title = {{Self-assembly formation of multiple DNA-tethered lipid bilayers}},
url = {{http://dx.doi.org/10.1016/j.jsb.2009.07.008}},
doi = {{10.1016/j.jsb.2009.07.008}},
volume = {{168}},
year = {{2009}},
}