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Self-assembly formation of multiple DNA-tethered lipid bilayers

Tabaei, Seyed R ; Jönsson, Peter LU ; Brändén, Magnus and Höök, Fredrik LU (2009) In Journal of Structural Biology 168(1). p.6-200
Abstract

Inspired by natural cell-cell junctions, where membrane-residing proteins control the separation between two or more membranes without interfering with their integrity, we report a new self-assembly route for formation of multiple highly fluid tethered lipid bilayers with the inter-membrane volume geometrically confined by membrane-anchored DNA duplexes. The formation of multiple planar membrane-membrane junctions were accomplished using disk shaped bicelles, composed of a mixture of the long-chained dimyristoyl phosphatidylcholine (DMPC) and the short-chained dihexanoyl PC further stabilized with the positively charged detergent hexadecyl-trimethyl-ammonium bromide (CTAB). Quartz crystal microbalance with dissipation (QCM-D) monitoring... (More)

Inspired by natural cell-cell junctions, where membrane-residing proteins control the separation between two or more membranes without interfering with their integrity, we report a new self-assembly route for formation of multiple highly fluid tethered lipid bilayers with the inter-membrane volume geometrically confined by membrane-anchored DNA duplexes. The formation of multiple planar membrane-membrane junctions were accomplished using disk shaped bicelles, composed of a mixture of the long-chained dimyristoyl phosphatidylcholine (DMPC) and the short-chained dihexanoyl PC further stabilized with the positively charged detergent hexadecyl-trimethyl-ammonium bromide (CTAB). Quartz crystal microbalance with dissipation (QCM-D) monitoring and fluorescence microscopy and fluorescence recovery after photobleaching (FRAP) were used to monitor the formation and to characterize the integrity of the self-assembled lipid-DNA architecture.

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author
; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
DNA, Dimyristoylphosphatidylcholine, Lipid Bilayers, Models, Theoretical, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Structural Biology
volume
168
issue
1
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:69249216546
  • pmid:19607925
ISSN
1095-8657
DOI
10.1016/j.jsb.2009.07.008
language
English
LU publication?
no
id
40915abb-7145-47e5-99d2-460405833b9f
date added to LUP
2018-01-26 10:51:17
date last changed
2020-09-02 03:47:53
@article{40915abb-7145-47e5-99d2-460405833b9f,
  abstract     = {<p>Inspired by natural cell-cell junctions, where membrane-residing proteins control the separation between two or more membranes without interfering with their integrity, we report a new self-assembly route for formation of multiple highly fluid tethered lipid bilayers with the inter-membrane volume geometrically confined by membrane-anchored DNA duplexes. The formation of multiple planar membrane-membrane junctions were accomplished using disk shaped bicelles, composed of a mixture of the long-chained dimyristoyl phosphatidylcholine (DMPC) and the short-chained dihexanoyl PC further stabilized with the positively charged detergent hexadecyl-trimethyl-ammonium bromide (CTAB). Quartz crystal microbalance with dissipation (QCM-D) monitoring and fluorescence microscopy and fluorescence recovery after photobleaching (FRAP) were used to monitor the formation and to characterize the integrity of the self-assembled lipid-DNA architecture.</p>},
  author       = {Tabaei, Seyed R and Jönsson, Peter and Brändén, Magnus and Höök, Fredrik},
  issn         = {1095-8657},
  language     = {eng},
  number       = {1},
  pages        = {6--200},
  publisher    = {Elsevier},
  series       = {Journal of Structural Biology},
  title        = {Self-assembly formation of multiple DNA-tethered lipid bilayers},
  url          = {http://dx.doi.org/10.1016/j.jsb.2009.07.008},
  doi          = {10.1016/j.jsb.2009.07.008},
  volume       = {168},
  year         = {2009},
}