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A novel approach for characterization of exopolymeric material in sewage sludge

Dey, Estera ; Szewczyk, Emil ; Wawrzynczyk, Joanna and Norrlöw, Olof (2006) In Journal of Residuals Science and Technology 3(2). p.97-103
Abstract
The digestibility of sludge is dependent on the content of organic extra-cellular polymeric substances. Currently the most appropriate and rapid assessment methods for the organic content of wastewater are the chemical oxygen demand and volatile solids values. These parameters also measure the non-biodegradable organics. We have developed a method to predict sewage sludge digestibility and the appropriate composition of enzymes needed. Sludge is treated with selected enzymes and the released products characterized using a lectin panel. One protease and several glycosidases were selected and applied under mild conditions to minimize lysis of cells in the sludge. Combinations of enzymes with and without sodium triphosphate pentabasic were... (More)
The digestibility of sludge is dependent on the content of organic extra-cellular polymeric substances. Currently the most appropriate and rapid assessment methods for the organic content of wastewater are the chemical oxygen demand and volatile solids values. These parameters also measure the non-biodegradable organics. We have developed a method to predict sewage sludge digestibility and the appropriate composition of enzymes needed. Sludge is treated with selected enzymes and the released products characterized using a lectin panel. One protease and several glycosidases were selected and applied under mild conditions to minimize lysis of cells in the sludge. Combinations of enzymes with and without sodium triphosphate pentabasic were studied. Partially enzymaticaly hydrolyzed extra-cellular polymeric substances and the released glycoconjugates were quantitatively determined by conventional methods. Surface plasmon resonance was used to investigate the interaction of extracts with a lectin panel. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Residuals Science and Technology
volume
3
issue
2
pages
97 - 103
publisher
DEStech Publications
external identifiers
  • wos:000237542900004
  • scopus:34250626118
ISSN
1544-8053
language
English
LU publication?
yes
id
40906912-ea9f-40e4-beb9-4976b64384c4 (old id 409523)
date added to LUP
2016-04-01 17:08:38
date last changed
2021-04-06 04:40:41
@article{40906912-ea9f-40e4-beb9-4976b64384c4,
  abstract     = {The digestibility of sludge is dependent on the content of organic extra-cellular polymeric substances. Currently the most appropriate and rapid assessment methods for the organic content of wastewater are the chemical oxygen demand and volatile solids values. These parameters also measure the non-biodegradable organics. We have developed a method to predict sewage sludge digestibility and the appropriate composition of enzymes needed. Sludge is treated with selected enzymes and the released products characterized using a lectin panel. One protease and several glycosidases were selected and applied under mild conditions to minimize lysis of cells in the sludge. Combinations of enzymes with and without sodium triphosphate pentabasic were studied. Partially enzymaticaly hydrolyzed extra-cellular polymeric substances and the released glycoconjugates were quantitatively determined by conventional methods. Surface plasmon resonance was used to investigate the interaction of extracts with a lectin panel.},
  author       = {Dey, Estera and Szewczyk, Emil and Wawrzynczyk, Joanna and Norrlöw, Olof},
  issn         = {1544-8053},
  language     = {eng},
  number       = {2},
  pages        = {97--103},
  publisher    = {DEStech Publications},
  series       = {Journal of Residuals Science and Technology},
  title        = {A novel approach for characterization of exopolymeric material in sewage sludge},
  volume       = {3},
  year         = {2006},
}