A combination of magnetic permeability detection with nanometer-scaled superparamagnetic tracer and its application for one-step detection of human urinary albumin in undiluted urine
(2006) In Biosensors & Bioelectronics 21(12). p.2248-2254- Abstract
- A rapid (6.5 min) and simple one-step magnetic immunoassay (MIA) has been developed for analysis of human urinary albumin in near patient settings. Polyclonal rabbit anti-human albumin was used as a capture antibody and monoclonal mouse anti-human albumin as a detection antibody in a two-site immunometric assay requiring no additional washing procedures. The polyclonal anti-human albumin was conjugated to silica microparticles (solid phase) and the monoclonal antibody to dextran-coated nanoscaled superparamagnetic particles (tracer). Quantification of human albumin in undiluted urine was performed by adding 2 mu L urine to a measuring vial containing solid-phase, superpararnagnetic tracer and reaction buffer and then inverting the vial by... (More)
- A rapid (6.5 min) and simple one-step magnetic immunoassay (MIA) has been developed for analysis of human urinary albumin in near patient settings. Polyclonal rabbit anti-human albumin was used as a capture antibody and monoclonal mouse anti-human albumin as a detection antibody in a two-site immunometric assay requiring no additional washing procedures. The polyclonal anti-human albumin was conjugated to silica microparticles (solid phase) and the monoclonal antibody to dextran-coated nanoscaled superparamagnetic particles (tracer). Quantification of human albumin in undiluted urine was performed by adding 2 mu L urine to a measuring vial containing solid-phase, superpararnagnetic tracer and reaction buffer and then inverting the vial by hand for 20 s. The measuring vial was allowed to stand for 6 min prior to detection, in order for the solid-phase sediment to form at the bottom of the vial. Lastly, the measuring vial was placed into a magnetic permeability detector, which measured the enrichment of superparamagnetic tracer in the sediment due to complex formation with human albumin. Total analysis time was 6.5 min. A linear response was obtained for 0-400 mg/L albumin with a detection limit of 5 mg/L. The total coefficient of variation (CV) was 11% calculated from four consecutive runs on a urine sample containing 11.1 mg/L human albumin during 3 consecutive days. Human urinary albumin analysis was performed on 149 patient samples using the MIA technique and the obtained results showed good correlation with the hospital immunoturbidimetric reference method (y = 1.004x + 4.01, R-2 = 0.978, N= 149) and a commercially available point of care albumin analysis provided by HemoCue Inc. (y = 0.98x + 5.8, R-2 = 0.833, N= 90). (c) 2005 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/409758
- author
- Lu, M ; Ibraimi, Filiz LU ; Kriz, D and Kriz, Kirstin LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- tracer, superparamagnetic, anti-human albumin-conjugated, albumin-conjugated silica particles, anti-human, magnetic immunoassay (MIA), human urinary albumin
- in
- Biosensors & Bioelectronics
- volume
- 21
- issue
- 12
- pages
- 2248 - 2254
- publisher
- Elsevier
- external identifiers
-
- pmid:16386414
- wos:000237613700008
- scopus:33646104219
- pmid:16386414
- ISSN
- 1873-4235
- DOI
- 10.1016/j.bios.2005.11.008
- language
- English
- LU publication?
- yes
- id
- d1552e2d-54a2-4592-8b3f-a05e1696ab07 (old id 409758)
- date added to LUP
- 2016-04-01 16:48:37
- date last changed
- 2022-01-28 22:17:46
@article{d1552e2d-54a2-4592-8b3f-a05e1696ab07,
abstract = {{A rapid (6.5 min) and simple one-step magnetic immunoassay (MIA) has been developed for analysis of human urinary albumin in near patient settings. Polyclonal rabbit anti-human albumin was used as a capture antibody and monoclonal mouse anti-human albumin as a detection antibody in a two-site immunometric assay requiring no additional washing procedures. The polyclonal anti-human albumin was conjugated to silica microparticles (solid phase) and the monoclonal antibody to dextran-coated nanoscaled superparamagnetic particles (tracer). Quantification of human albumin in undiluted urine was performed by adding 2 mu L urine to a measuring vial containing solid-phase, superpararnagnetic tracer and reaction buffer and then inverting the vial by hand for 20 s. The measuring vial was allowed to stand for 6 min prior to detection, in order for the solid-phase sediment to form at the bottom of the vial. Lastly, the measuring vial was placed into a magnetic permeability detector, which measured the enrichment of superparamagnetic tracer in the sediment due to complex formation with human albumin. Total analysis time was 6.5 min. A linear response was obtained for 0-400 mg/L albumin with a detection limit of 5 mg/L. The total coefficient of variation (CV) was 11% calculated from four consecutive runs on a urine sample containing 11.1 mg/L human albumin during 3 consecutive days. Human urinary albumin analysis was performed on 149 patient samples using the MIA technique and the obtained results showed good correlation with the hospital immunoturbidimetric reference method (y = 1.004x + 4.01, R-2 = 0.978, N= 149) and a commercially available point of care albumin analysis provided by HemoCue Inc. (y = 0.98x + 5.8, R-2 = 0.833, N= 90). (c) 2005 Elsevier B.V. All rights reserved.}},
author = {{Lu, M and Ibraimi, Filiz and Kriz, D and Kriz, Kirstin}},
issn = {{1873-4235}},
keywords = {{tracer; superparamagnetic; anti-human albumin-conjugated; albumin-conjugated silica particles; anti-human; magnetic immunoassay (MIA); human urinary albumin}},
language = {{eng}},
number = {{12}},
pages = {{2248--2254}},
publisher = {{Elsevier}},
series = {{Biosensors & Bioelectronics}},
title = {{A combination of magnetic permeability detection with nanometer-scaled superparamagnetic tracer and its application for one-step detection of human urinary albumin in undiluted urine}},
url = {{http://dx.doi.org/10.1016/j.bios.2005.11.008}},
doi = {{10.1016/j.bios.2005.11.008}},
volume = {{21}},
year = {{2006}},
}