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A combination of magnetic permeability detection with nanometer-scaled superparamagnetic tracer and its application for one-step detection of human urinary albumin in undiluted urine

Lu, M ; Ibraimi, Filiz LU ; Kriz, D and Kriz, Kirstin LU (2006) In Biosensors & Bioelectronics 21(12). p.2248-2254
Abstract
A rapid (6.5 min) and simple one-step magnetic immunoassay (MIA) has been developed for analysis of human urinary albumin in near patient settings. Polyclonal rabbit anti-human albumin was used as a capture antibody and monoclonal mouse anti-human albumin as a detection antibody in a two-site immunometric assay requiring no additional washing procedures. The polyclonal anti-human albumin was conjugated to silica microparticles (solid phase) and the monoclonal antibody to dextran-coated nanoscaled superparamagnetic particles (tracer). Quantification of human albumin in undiluted urine was performed by adding 2 mu L urine to a measuring vial containing solid-phase, superpararnagnetic tracer and reaction buffer and then inverting the vial by... (More)
A rapid (6.5 min) and simple one-step magnetic immunoassay (MIA) has been developed for analysis of human urinary albumin in near patient settings. Polyclonal rabbit anti-human albumin was used as a capture antibody and monoclonal mouse anti-human albumin as a detection antibody in a two-site immunometric assay requiring no additional washing procedures. The polyclonal anti-human albumin was conjugated to silica microparticles (solid phase) and the monoclonal antibody to dextran-coated nanoscaled superparamagnetic particles (tracer). Quantification of human albumin in undiluted urine was performed by adding 2 mu L urine to a measuring vial containing solid-phase, superpararnagnetic tracer and reaction buffer and then inverting the vial by hand for 20 s. The measuring vial was allowed to stand for 6 min prior to detection, in order for the solid-phase sediment to form at the bottom of the vial. Lastly, the measuring vial was placed into a magnetic permeability detector, which measured the enrichment of superparamagnetic tracer in the sediment due to complex formation with human albumin. Total analysis time was 6.5 min. A linear response was obtained for 0-400 mg/L albumin with a detection limit of 5 mg/L. The total coefficient of variation (CV) was 11% calculated from four consecutive runs on a urine sample containing 11.1 mg/L human albumin during 3 consecutive days. Human urinary albumin analysis was performed on 149 patient samples using the MIA technique and the obtained results showed good correlation with the hospital immunoturbidimetric reference method (y = 1.004x + 4.01, R-2 = 0.978, N= 149) and a commercially available point of care albumin analysis provided by HemoCue Inc. (y = 0.98x + 5.8, R-2 = 0.833, N= 90). (c) 2005 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
tracer, superparamagnetic, anti-human albumin-conjugated, albumin-conjugated silica particles, anti-human, magnetic immunoassay (MIA), human urinary albumin
in
Biosensors & Bioelectronics
volume
21
issue
12
pages
2248 - 2254
publisher
Elsevier
external identifiers
  • pmid:16386414
  • wos:000237613700008
  • scopus:33646104219
  • pmid:16386414
ISSN
1873-4235
DOI
10.1016/j.bios.2005.11.008
language
English
LU publication?
yes
id
d1552e2d-54a2-4592-8b3f-a05e1696ab07 (old id 409758)
date added to LUP
2016-04-01 16:48:37
date last changed
2021-08-25 05:00:16
@article{d1552e2d-54a2-4592-8b3f-a05e1696ab07,
  abstract     = {A rapid (6.5 min) and simple one-step magnetic immunoassay (MIA) has been developed for analysis of human urinary albumin in near patient settings. Polyclonal rabbit anti-human albumin was used as a capture antibody and monoclonal mouse anti-human albumin as a detection antibody in a two-site immunometric assay requiring no additional washing procedures. The polyclonal anti-human albumin was conjugated to silica microparticles (solid phase) and the monoclonal antibody to dextran-coated nanoscaled superparamagnetic particles (tracer). Quantification of human albumin in undiluted urine was performed by adding 2 mu L urine to a measuring vial containing solid-phase, superpararnagnetic tracer and reaction buffer and then inverting the vial by hand for 20 s. The measuring vial was allowed to stand for 6 min prior to detection, in order for the solid-phase sediment to form at the bottom of the vial. Lastly, the measuring vial was placed into a magnetic permeability detector, which measured the enrichment of superparamagnetic tracer in the sediment due to complex formation with human albumin. Total analysis time was 6.5 min. A linear response was obtained for 0-400 mg/L albumin with a detection limit of 5 mg/L. The total coefficient of variation (CV) was 11% calculated from four consecutive runs on a urine sample containing 11.1 mg/L human albumin during 3 consecutive days. Human urinary albumin analysis was performed on 149 patient samples using the MIA technique and the obtained results showed good correlation with the hospital immunoturbidimetric reference method (y = 1.004x + 4.01, R-2 = 0.978, N= 149) and a commercially available point of care albumin analysis provided by HemoCue Inc. (y = 0.98x + 5.8, R-2 = 0.833, N= 90). (c) 2005 Elsevier B.V. All rights reserved.},
  author       = {Lu, M and Ibraimi, Filiz and Kriz, D and Kriz, Kirstin},
  issn         = {1873-4235},
  language     = {eng},
  number       = {12},
  pages        = {2248--2254},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {A combination of magnetic permeability detection with nanometer-scaled superparamagnetic tracer and its application for one-step detection of human urinary albumin in undiluted urine},
  url          = {http://dx.doi.org/10.1016/j.bios.2005.11.008},
  doi          = {10.1016/j.bios.2005.11.008},
  volume       = {21},
  year         = {2006},
}