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Effect of modified Fenton's reaction on microbial activity and removal of PAHs in creosote oil contaminated soil

Palmroth, Marja LU ; Langwaldt, JH ; Aunola, TA ; Goi, A ; Munster, U ; Puhakka, JA and Tuhkanen, A (2006) In Biodegradation 17(2). p.29-39
Abstract
This study describes the removal of polycyclic aromatic hydrocarbons (PAHs) from creosote oil contaminated soil by modified Fenton's reaction in laboratory-scale column experiments and subsequent aerobic biodegradation of PAHs by indigenous bacteria during incubation of the soil. The effect of hydrogen peroxide addition for 4 and 10 days and saturation of soil with H2O2 on was studied. In both experiments the H2O2 dosage was 0.4 g H2O2/g soil. In completely H2O2-saturated soil the removal of PAHs (44% within 4 days) by modified Fenton reaction was uniform over the entire soil column. In non-uniformly saturated soil, PAH removal was higher in completely saturated soil (52% in 10 days) compared to partially saturated soil, with only 25% in... (More)
This study describes the removal of polycyclic aromatic hydrocarbons (PAHs) from creosote oil contaminated soil by modified Fenton's reaction in laboratory-scale column experiments and subsequent aerobic biodegradation of PAHs by indigenous bacteria during incubation of the soil. The effect of hydrogen peroxide addition for 4 and 10 days and saturation of soil with H2O2 on was studied. In both experiments the H2O2 dosage was 0.4 g H2O2/g soil. In completely H2O2-saturated soil the removal of PAHs (44% within 4 days) by modified Fenton reaction was uniform over the entire soil column. In non-uniformly saturated soil, PAH removal was higher in completely saturated soil (52% in 10 days) compared to partially saturated soil, with only 25% in 10 days. The effect of the modified Fenton's reaction on the microbial activity in the soil was assessed based on toxicity tests towards Vibrio fischeri, enumeration of viable and dead cells, microbial extracellular enzyme activity, and oxygen consumption and carbon dioxide production during soil incubation. During the laboratory-scale column experiments, the toxicity of column leachate towards Vibrio fischeri increased as a result of the modified Fenton's reaction. The activities of the microbial extracellular enzymes acetate- and acidic phosphomono-esterase were lower in the incubated modified Fenton's treated soil compared to extracellular enzyme activities in untreated soil. Abundance of viable cells was lower in incubated modified Fenton treated soil than in untreated soil. Incubation of soil in serum bottles at 20 degrees C resulted in consumption of oxygen and formation of carbon dioxide, indicating aerobic biodegradation of organic compounds. In untreated soil 20-30% of the PAHs were biodegraded during 2 months of incubation. Incubation of chemically treated soil slightly increased PAH-removal compared to PAH-removal in untreated soil. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
in situ, Fenton, extracellular enzymes, biodegradation, creosote oil, polycyclic aromatic hydrocarbons
in
Biodegradation
volume
17
issue
2
pages
29 - 39
publisher
Springer
external identifiers
  • pmid:16456613
  • wos:000237467600005
  • scopus:33645284152
ISSN
0923-9820
DOI
10.1007/s10532-005-6060-3
language
English
LU publication?
yes
id
c1091285-f4e1-435b-a50f-29bb5728a524 (old id 409775)
date added to LUP
2016-04-01 16:01:53
date last changed
2021-09-29 02:17:09
@article{c1091285-f4e1-435b-a50f-29bb5728a524,
  abstract     = {This study describes the removal of polycyclic aromatic hydrocarbons (PAHs) from creosote oil contaminated soil by modified Fenton's reaction in laboratory-scale column experiments and subsequent aerobic biodegradation of PAHs by indigenous bacteria during incubation of the soil. The effect of hydrogen peroxide addition for 4 and 10 days and saturation of soil with H2O2 on was studied. In both experiments the H2O2 dosage was 0.4 g H2O2/g soil. In completely H2O2-saturated soil the removal of PAHs (44% within 4 days) by modified Fenton reaction was uniform over the entire soil column. In non-uniformly saturated soil, PAH removal was higher in completely saturated soil (52% in 10 days) compared to partially saturated soil, with only 25% in 10 days. The effect of the modified Fenton's reaction on the microbial activity in the soil was assessed based on toxicity tests towards Vibrio fischeri, enumeration of viable and dead cells, microbial extracellular enzyme activity, and oxygen consumption and carbon dioxide production during soil incubation. During the laboratory-scale column experiments, the toxicity of column leachate towards Vibrio fischeri increased as a result of the modified Fenton's reaction. The activities of the microbial extracellular enzymes acetate- and acidic phosphomono-esterase were lower in the incubated modified Fenton's treated soil compared to extracellular enzyme activities in untreated soil. Abundance of viable cells was lower in incubated modified Fenton treated soil than in untreated soil. Incubation of soil in serum bottles at 20 degrees C resulted in consumption of oxygen and formation of carbon dioxide, indicating aerobic biodegradation of organic compounds. In untreated soil 20-30% of the PAHs were biodegraded during 2 months of incubation. Incubation of chemically treated soil slightly increased PAH-removal compared to PAH-removal in untreated soil.},
  author       = {Palmroth, Marja and Langwaldt, JH and Aunola, TA and Goi, A and Munster, U and Puhakka, JA and Tuhkanen, A},
  issn         = {0923-9820},
  language     = {eng},
  number       = {2},
  pages        = {29--39},
  publisher    = {Springer},
  series       = {Biodegradation},
  title        = {Effect of modified Fenton's reaction on microbial activity and removal of PAHs in creosote oil contaminated soil},
  url          = {http://dx.doi.org/10.1007/s10532-005-6060-3},
  doi          = {10.1007/s10532-005-6060-3},
  volume       = {17},
  year         = {2006},
}