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Optical Analysis of biological media - continuous wave diffuse spectroscopy

Sørensen Dam, Jan LU (2000) In Lund Reports in Atomic Physics LRAP-265.
Abstract
The main topic of this thesis is real-time quantification of relevant chromophores and light scattering elements in biological media. The presented methods and instrumentation are based on continuous wave (steady-state) optical measurements of (a) spatially-resolved diffuse reflectance from bulk media and (b) combined spatially-resolved and goniometric measurements of re-emitted light from thin samples. These two configurations address applications for non-invasive medical diagnostics (optical biopsy), and in vitro diffuse spectroscopy of turbid samples (e.g. whole blood analysis and immunoassays), respectively. The physical basis of light-tissue interaction, i.e. absorption and scattering, is discussed, as well as various theoretical... (More)
The main topic of this thesis is real-time quantification of relevant chromophores and light scattering elements in biological media. The presented methods and instrumentation are based on continuous wave (steady-state) optical measurements of (a) spatially-resolved diffuse reflectance from bulk media and (b) combined spatially-resolved and goniometric measurements of re-emitted light from thin samples. These two configurations address applications for non-invasive medical diagnostics (optical biopsy), and in vitro diffuse spectroscopy of turbid samples (e.g. whole blood analysis and immunoassays), respectively. The physical basis of light-tissue interaction, i.e. absorption and scattering, is discussed, as well as various theoretical models for light propagation in turbid biological media, e.g. Monte Carlo simulations, diffusion theory, and the adding-doubling method. The optical properties are extracted from the measurements using multivariate calibration and analysis techniques. Therefore, a general introduction to such methods is also included, e.g. principal component analysis, multiple polynomial regression, and Newton-Raphson prediction algorithms. Finally, some of the prototype instrumentation developed during the project is presented, e.g. a fiber probe system, an integrating sphere setup, and a hybrid goniometric/spatially-resolved system. (Less)
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • Dr Sterenborg, HJCM, Department of Radiotherapy, Academic Hospital Rotterdam
organization
publishing date
type
Thesis
publication status
published
subject
keywords
spatially resolved diffuse reflectance, in vitro diffuse spectroscopy, continuous wave techniques, Nuclear physics, multivariate data analysis., Kärnfysik, Fysik, Physics, Medicin (människa och djur), Medicine (human and vertebrates), Monte Carlo simulations, light propagation models, multiple scattering, Tissue optics, tissue optical properties, Fysicumarkivet A:2000:Sörensen Dam
in
Lund Reports in Atomic Physics
volume
LRAP-265
pages
176 pages
publisher
Department of Physics, Lund University
defense location
Hörsal B, Dept. of Physics
defense date
2000-12-15 10:15:00
external identifiers
  • other:ISRN: LUTFD2(TFAF-1045)1-91 (2000)
ISSN
0281-2762
ISBN
91-628-4546-2
language
English
LU publication?
yes
additional info
J.S. Dam, P.E. Andersen, T. Dalgaard and P.E. Fabricius, Determination of tissue optical properties from diffuse reflectance profiles by multivariate calibration, Appl. Opt. 37, 772-778 (1998).
J.S. Dam, T. Dalgaard, P.E. Fabricius and S. Andersson-Engels, Multiple polynomial regression method for determination of biomedical optical properties from integrating sphere measurements, Appl. Opt. 39, 1202-1209 (2000).
J.S. Dam, C.B. Pedersen, T. Dalgaard, P. Aruna and S. Andersson-Engels, Fiber optic probe for non-invasive real-time determination of tissue optical properties at multiple wavelengths, Appl. Opt. (Accepted, Oct. 2000).
T.H. Pham, F. Bevilacqua, T. Spott, J.S. Dam, B.J. Tromberg and S. Andersson-Engels, Quantifying the absorption and reduced scattering coefficients of tissue-like turbid media over a broad spectral range using a non-contact Fourier interferometric, hyperspectral imaging system, Appl. Opt. (In press, vol. 39 2000).
J.S. Dam, C.B. Pedersen, T. Dalgaard, P.E. Fabricius and S. Andersson-Engels, Monte Carlo study on optical characterization of thin turbid samples using angular and spatially- resolved measurements. (Manuscript for Appl. Opt.).
P.E. Andersen, J.S. Dam, P.M. Petersen and P. Bjerring, Local diffuse reflectance from three-layered skin tissue structures, "Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model, and Human Studies II", SPIE Proc. 2979, 515-526 (1997).
J. Swartling, C. Klinteberg, J.S. Dam, T. Johansson, J. Roth, and S. Andersson-Engels. Comparison of spatially and temporally resolved diffuse reflectance measurement systems for determination of biomedical optical properties at 785 nm, (Manuscript in preparation)
id
b4d2188c-7bd5-4adb-97a0-12225f04cc98 (old id 41154)
date added to LUP
2016-04-04 11:51:52
date last changed
2019-05-21 17:53:08
@phdthesis{b4d2188c-7bd5-4adb-97a0-12225f04cc98,
  abstract     = {The main topic of this thesis is real-time quantification of relevant chromophores and light scattering elements in biological media. The presented methods and instrumentation are based on continuous wave (steady-state) optical measurements of (a) spatially-resolved diffuse reflectance from bulk media and (b) combined spatially-resolved and goniometric measurements of re-emitted light from thin samples. These two configurations address applications for non-invasive medical diagnostics (optical biopsy), and in vitro diffuse spectroscopy of turbid samples (e.g. whole blood analysis and immunoassays), respectively. The physical basis of light-tissue interaction, i.e. absorption and scattering, is discussed, as well as various theoretical models for light propagation in turbid biological media, e.g. Monte Carlo simulations, diffusion theory, and the adding-doubling method. The optical properties are extracted from the measurements using multivariate calibration and analysis techniques. Therefore, a general introduction to such methods is also included, e.g. principal component analysis, multiple polynomial regression, and Newton-Raphson prediction algorithms. Finally, some of the prototype instrumentation developed during the project is presented, e.g. a fiber probe system, an integrating sphere setup, and a hybrid goniometric/spatially-resolved system.},
  author       = {Sørensen Dam, Jan},
  isbn         = {91-628-4546-2},
  issn         = {0281-2762},
  language     = {eng},
  publisher    = {Department of Physics, Lund University},
  school       = {Lund University},
  series       = {Lund Reports in Atomic Physics},
  title        = {Optical Analysis of biological media - continuous wave diffuse spectroscopy},
  url          = {https://lup.lub.lu.se/search/ws/files/5872911/2299924.pdf},
  volume       = {LRAP-265},
  year         = {2000},
}