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Probiotic properties of exopolysaccharide producing lactic acid bacteria isolated from vegetables and traditional Indian fermented foods

Patel, Ami LU ; Lindström, Cecilia LU ; Patel, Arti; Prajapati, Jashbai and Holst, Olle LU (2012) In International Journal of Fermented Foods 1(1). p.87-101
Abstract
A total of 203 lactic acid bacteria (LAB) isolated from vegetables and traditional

fermented food products of India such as idli batter, dhokla batter and dahi were

screened for exopolysaccharide (EPS) production. Based on the amount of EPS

produced, 17 LAB isolates were selected for biochemical and genetic

characterization using 16S rRNA gene sequencing. The isolates were belonging

to the genera Lactobacillus, Weissella and Pediococcus. In vitro examination

was performed to evaluate their probiotic potential. Resistance to low pH and 0.3

% bile salts was studied. Bile salt hydrolase (BSH) activity, susceptibility to

various antibiotics and antimicrobial activity... (More)
A total of 203 lactic acid bacteria (LAB) isolated from vegetables and traditional

fermented food products of India such as idli batter, dhokla batter and dahi were

screened for exopolysaccharide (EPS) production. Based on the amount of EPS

produced, 17 LAB isolates were selected for biochemical and genetic

characterization using 16S rRNA gene sequencing. The isolates were belonging

to the genera Lactobacillus, Weissella and Pediococcus. In vitro examination

was performed to evaluate their probiotic potential. Resistance to low pH and 0.3

% bile salts was studied. Bile salt hydrolase (BSH) activity, susceptibility to

various antibiotics and antimicrobial activity against the non pathogenic E. coli

K12 were also investigated. The results showed that the isolates could not grow

in the presence of oxbile however some survived exposure to it for 2.5 h. Some

isolates were able to grow in the presence of sodium taurocholate and showed

considerable antimicrobial activity against E. coli K12. Five isolates were showing

BSH activity which is reported for the genus Weissella for the first time. The

results suggest that traditional fermented products could be an alternative and

readily available (Less)
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organization
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Contribution to journal
publication status
published
subject
keywords
Exopolysaccharides, BSH activity, Weissella, dhokla batter, idli batter, antimicrobial activity
in
International Journal of Fermented Foods
volume
1
issue
1
pages
87 - 101
publisher
New Delhi Publishers
ISSN
2321-712X
language
English
LU publication?
yes
id
0e6f0ea0-580c-4cbb-9c45-a71c75795fde (old id 4124589)
date added to LUP
2013-11-04 11:17:01
date last changed
2016-04-15 21:24:57
@article{0e6f0ea0-580c-4cbb-9c45-a71c75795fde,
  abstract     = {A total of 203 lactic acid bacteria (LAB) isolated from vegetables and traditional<br/><br>
fermented food products of India such as idli batter, dhokla batter and dahi were<br/><br>
screened for exopolysaccharide (EPS) production. Based on the amount of EPS<br/><br>
produced, 17 LAB isolates were selected for biochemical and genetic<br/><br>
characterization using 16S rRNA gene sequencing. The isolates were belonging<br/><br>
to the genera Lactobacillus, Weissella and Pediococcus. In vitro examination<br/><br>
was performed to evaluate their probiotic potential. Resistance to low pH and 0.3<br/><br>
% bile salts was studied. Bile salt hydrolase (BSH) activity, susceptibility to<br/><br>
various antibiotics and antimicrobial activity against the non pathogenic E. coli<br/><br>
K12 were also investigated. The results showed that the isolates could not grow<br/><br>
in the presence of oxbile however some survived exposure to it for 2.5 h. Some<br/><br>
isolates were able to grow in the presence of sodium taurocholate and showed<br/><br>
considerable antimicrobial activity against E. coli K12. Five isolates were showing<br/><br>
BSH activity which is reported for the genus Weissella for the first time. The<br/><br>
results suggest that traditional fermented products could be an alternative and<br/><br>
readily available},
  author       = {Patel, Ami and Lindström, Cecilia and Patel, Arti and Prajapati, Jashbai and Holst, Olle},
  issn         = {2321-712X},
  keyword      = {Exopolysaccharides,BSH activity,Weissella,dhokla batter,idli batter,antimicrobial activity},
  language     = {eng},
  number       = {1},
  pages        = {87--101},
  publisher    = {New Delhi Publishers},
  series       = {International Journal of Fermented Foods},
  title        = {Probiotic properties of exopolysaccharide producing lactic acid bacteria isolated from vegetables and traditional Indian fermented foods},
  volume       = {1},
  year         = {2012},
}