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Expression profiling of Wilms tumors reveals new candidate genes for different clinical parameters

Zirn, B ; Hartmann, O ; Samans, B ; Krause, M ; Wittmann, S ; Mertens, Fredrik LU ; Graf, N ; Eilers, M and Gessler, M (2006) In International Journal of Cancer 118(8). p.1954-1962
Abstract
Wilms tumor is the most frequent renal neoplasm in children, but our understanding of its genetic basis is still limited. We performed cDNA microarray experiments using 63 primary Wilms tumors with the aim of detecting new candidate genes associated with malignancy grade and tumor progression. All tumors had received preoperative chemotherapy as mandated by the SIOP protocol, which sets this study apart from related approaches in the Unites States that are based on untreated samples. The stratification of expression data according to clinical criteria allowed a rather clear distinction between different subsets of Wilms tumors. Clear-cut differences in expression patterns were discovered between relapse-free as opposed to relapsed tumors... (More)
Wilms tumor is the most frequent renal neoplasm in children, but our understanding of its genetic basis is still limited. We performed cDNA microarray experiments using 63 primary Wilms tumors with the aim of detecting new candidate genes associated with malignancy grade and tumor progression. All tumors had received preoperative chemotherapy as mandated by the SIOP protocol, which sets this study apart from related approaches in the Unites States that are based on untreated samples. The stratification of expression data according to clinical criteria allowed a rather clear distinction between different subsets of Wilms tumors. Clear-cut differences in expression patterns were discovered between relapse-free as opposed to relapsed tumors and tumors with intermediate risk as opposed to high risk histology. Several differentially expressed genes, e.g. TRIM22, CENPF, MYCN, CTGF, RARRES3 and EZH2, were associated with Wilms tumor progression. For a subset of differentially expressed genes, microarray data were confirmed by real-time RT-PCR on the original set of tumors. Interestingly, we found the retinoic acid pathway to be deregulated at different levels in advanced tumors suggesting that treatment of these tumors with retinoic acid may represent a promising novel therapeutic approach. (c) 2005 Wiley-Liss, Inc. (Less)
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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
E2F, microarray, retinoic acid, Wilms tumor, nephroblastoma
in
International Journal of Cancer
volume
118
issue
8
pages
1954 - 1962
publisher
John Wiley and Sons
external identifiers
  • wos:000236397200015
  • pmid:16287080
  • scopus:33645236966
ISSN
0020-7136
DOI
10.1002/ijc.21564
language
English
LU publication?
yes
id
9f35400f-5c56-45f6-b252-625d8cf4962d (old id 414832)
date added to LUP
2016-04-01 11:46:05
date last changed
2021-01-12 04:05:10
@article{9f35400f-5c56-45f6-b252-625d8cf4962d,
  abstract     = {Wilms tumor is the most frequent renal neoplasm in children, but our understanding of its genetic basis is still limited. We performed cDNA microarray experiments using 63 primary Wilms tumors with the aim of detecting new candidate genes associated with malignancy grade and tumor progression. All tumors had received preoperative chemotherapy as mandated by the SIOP protocol, which sets this study apart from related approaches in the Unites States that are based on untreated samples. The stratification of expression data according to clinical criteria allowed a rather clear distinction between different subsets of Wilms tumors. Clear-cut differences in expression patterns were discovered between relapse-free as opposed to relapsed tumors and tumors with intermediate risk as opposed to high risk histology. Several differentially expressed genes, e.g. TRIM22, CENPF, MYCN, CTGF, RARRES3 and EZH2, were associated with Wilms tumor progression. For a subset of differentially expressed genes, microarray data were confirmed by real-time RT-PCR on the original set of tumors. Interestingly, we found the retinoic acid pathway to be deregulated at different levels in advanced tumors suggesting that treatment of these tumors with retinoic acid may represent a promising novel therapeutic approach. (c) 2005 Wiley-Liss, Inc.},
  author       = {Zirn, B and Hartmann, O and Samans, B and Krause, M and Wittmann, S and Mertens, Fredrik and Graf, N and Eilers, M and Gessler, M},
  issn         = {0020-7136},
  language     = {eng},
  number       = {8},
  pages        = {1954--1962},
  publisher    = {John Wiley and Sons},
  series       = {International Journal of Cancer},
  title        = {Expression profiling of Wilms tumors reveals new candidate genes for different clinical parameters},
  url          = {http://dx.doi.org/10.1002/ijc.21564},
  doi          = {10.1002/ijc.21564},
  volume       = {118},
  year         = {2006},
}