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Ex-Vivo Force Spectroscopy of Intestinal Mucosa Reveals the Mechanical Properties of Mucus Blankets

Sotres, Javier; Jankovskaja, Skaidre LU ; Wannerberger, Kristin and Arnebrant, Thomas (2017) In Scientific Reports 7(1).
Abstract

Mucus is the viscous gel that protects mucosal surfaces. It also plays a crucial role in several diseases as well as in mucosal drug delivery. Because of technical limitations, mucus properties have mainly been addressed by in-vitro studies. However, this approach can lead to artifacts as mucus collection can alter its structure. Here we show that by using an implemented atomic force microscope it is possible to measure the interactions between micro-particles and mucus blankets ex-vivo i.e., on fresh excised mucus-covered tissues. By applying this method to study the small intestine, we were able to quantify the stiffness and adhesiveness of its mucus blanket at different pH values. We also demonstrate the ability of mucus blankets to... (More)

Mucus is the viscous gel that protects mucosal surfaces. It also plays a crucial role in several diseases as well as in mucosal drug delivery. Because of technical limitations, mucus properties have mainly been addressed by in-vitro studies. However, this approach can lead to artifacts as mucus collection can alter its structure. Here we show that by using an implemented atomic force microscope it is possible to measure the interactions between micro-particles and mucus blankets ex-vivo i.e., on fresh excised mucus-covered tissues. By applying this method to study the small intestine, we were able to quantify the stiffness and adhesiveness of its mucus blanket at different pH values. We also demonstrate the ability of mucus blankets to bind and attract particles hundreds of μm away from their surface, and to trap and bury them even if their size is as big as 15 μm.

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author
publishing date
type
Contribution to journal
publication status
epub
in
Scientific Reports
volume
7
issue
1
publisher
Nature Publishing Group
external identifiers
  • scopus:85026815728
ISSN
2045-2322
DOI
10.1038/s41598-017-07552-7
language
English
LU publication?
no
id
414c2a1c-38ec-4103-82e0-72385c56bfde
date added to LUP
2017-08-24 15:06:57
date last changed
2017-08-25 03:00:02
@article{414c2a1c-38ec-4103-82e0-72385c56bfde,
  abstract     = {<p>Mucus is the viscous gel that protects mucosal surfaces. It also plays a crucial role in several diseases as well as in mucosal drug delivery. Because of technical limitations, mucus properties have mainly been addressed by in-vitro studies. However, this approach can lead to artifacts as mucus collection can alter its structure. Here we show that by using an implemented atomic force microscope it is possible to measure the interactions between micro-particles and mucus blankets ex-vivo i.e., on fresh excised mucus-covered tissues. By applying this method to study the small intestine, we were able to quantify the stiffness and adhesiveness of its mucus blanket at different pH values. We also demonstrate the ability of mucus blankets to bind and attract particles hundreds of μm away from their surface, and to trap and bury them even if their size is as big as 15 μm.</p>},
  articleno    = {7270},
  author       = {Sotres, Javier and Jankovskaja, Skaidre and Wannerberger, Kristin and Arnebrant, Thomas},
  issn         = {2045-2322},
  language     = {eng},
  month        = {08},
  number       = {1},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Ex-Vivo Force Spectroscopy of Intestinal Mucosa Reveals the Mechanical Properties of Mucus Blankets},
  url          = {http://dx.doi.org/10.1038/s41598-017-07552-7},
  volume       = {7},
  year         = {2017},
}