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Yeast ribosomal P0 protein has two separate binding sites for P1/P2 proteins

Krokowski, D ; Boguszewska, A ; Abramczyk, D ; Liljas, Anders LU ; Tchorzewski, Marek LU and Grankowski, N (2006) In Molecular Microbiology 60(2). p.386-400
Abstract
The ribosome has a distinct lateral protuberance called the stalk; in eukaryotes it is formed by the acidic ribosomal P-proteins which are organized as a pentameric entity described as P0-(P1-P2)(2). Bilateral interactions between P0 and P1/P2 proteins have been studied extensively, however, the region on P0 responsible for the binding of P1/P2 proteins has not been precisely defined. Here we report a study which takes the current knowledge of the P0 - P1/P2 protein interaction beyond the recently published information. Using truncated forms of P0 protein and several in vitro and in vivo approaches, we have defined the region between positions 199 and 258 as the P0 protein fragment responsible for the binding of P1/P2 proteins in the yeast... (More)
The ribosome has a distinct lateral protuberance called the stalk; in eukaryotes it is formed by the acidic ribosomal P-proteins which are organized as a pentameric entity described as P0-(P1-P2)(2). Bilateral interactions between P0 and P1/P2 proteins have been studied extensively, however, the region on P0 responsible for the binding of P1/P2 proteins has not been precisely defined. Here we report a study which takes the current knowledge of the P0 - P1/P2 protein interaction beyond the recently published information. Using truncated forms of P0 protein and several in vitro and in vivo approaches, we have defined the region between positions 199 and 258 as the P0 protein fragment responsible for the binding of P1/P2 proteins in the yeast Saccharomyces cerevisiae. We show two short amino acid regions of P0 protein located at positions 199-230 and 231-258, to be responsible for independent binding of two dimers, P1A-P2B and P1B-P2A respectively. In addition, two elements, the sequence spanning amino acids 199-230 and the P1A-P2B dimer were found to be essential for stalk formation, indicating that this process is dependent on a balance between the P1A-P2B dimer and the P0 protein. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Microbiology
volume
60
issue
2
pages
386 - 400
publisher
Wiley-Blackwell
external identifiers
  • pmid:16573688
  • wos:000236328700012
  • scopus:33645281960
  • pmid:16573688
ISSN
1365-2958
DOI
10.1111/j.1365-2958.2006.05117.x
language
English
LU publication?
yes
id
72c82135-ae19-476c-9361-2c8ea074e58d (old id 415057)
date added to LUP
2016-04-01 12:02:16
date last changed
2021-09-01 04:04:13
@article{72c82135-ae19-476c-9361-2c8ea074e58d,
  abstract     = {The ribosome has a distinct lateral protuberance called the stalk; in eukaryotes it is formed by the acidic ribosomal P-proteins which are organized as a pentameric entity described as P0-(P1-P2)(2). Bilateral interactions between P0 and P1/P2 proteins have been studied extensively, however, the region on P0 responsible for the binding of P1/P2 proteins has not been precisely defined. Here we report a study which takes the current knowledge of the P0 - P1/P2 protein interaction beyond the recently published information. Using truncated forms of P0 protein and several in vitro and in vivo approaches, we have defined the region between positions 199 and 258 as the P0 protein fragment responsible for the binding of P1/P2 proteins in the yeast Saccharomyces cerevisiae. We show two short amino acid regions of P0 protein located at positions 199-230 and 231-258, to be responsible for independent binding of two dimers, P1A-P2B and P1B-P2A respectively. In addition, two elements, the sequence spanning amino acids 199-230 and the P1A-P2B dimer were found to be essential for stalk formation, indicating that this process is dependent on a balance between the P1A-P2B dimer and the P0 protein.},
  author       = {Krokowski, D and Boguszewska, A and Abramczyk, D and Liljas, Anders and Tchorzewski, Marek and Grankowski, N},
  issn         = {1365-2958},
  language     = {eng},
  number       = {2},
  pages        = {386--400},
  publisher    = {Wiley-Blackwell},
  series       = {Molecular Microbiology},
  title        = {Yeast ribosomal P0 protein has two separate binding sites for P1/P2 proteins},
  url          = {http://dx.doi.org/10.1111/j.1365-2958.2006.05117.x},
  doi          = {10.1111/j.1365-2958.2006.05117.x},
  volume       = {60},
  year         = {2006},
}