Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Automated flow enzyme-linked immunosorbent assay (ELISA) system for analysis of methyl parathion

Kumar, MA ; Chouhan, RS ; Thakur, MS ; Rani, BEA ; Mattiasson, Bo LU and Karanth, NG (2006) In Analytica Chimica Acta 560(1-2). p.30-34
Abstract
sensitive detection of pesticides is of utmost importance in environment pesticides in agricultural and environmental samples wherein antibodies are employed against the target molecules. Accurate diagnosis depends on the affinity and specificity of the antibody preparation used, and high affinity antibodies are essential for the detection of very small amounts of pesticides. Enzyme linked immuno sorbent assay (ELISA) coupled with flow injection analysis (FIA) technique provides a very high sensitivity with high throughput of analyses. Automation of this analysis scheme ensures precise detection with high accuracy. The present development aims at providing a user-friendly system for achieving this objective. It employs a 8952... (More)
sensitive detection of pesticides is of utmost importance in environment pesticides in agricultural and environmental samples wherein antibodies are employed against the target molecules. Accurate diagnosis depends on the affinity and specificity of the antibody preparation used, and high affinity antibodies are essential for the detection of very small amounts of pesticides. Enzyme linked immuno sorbent assay (ELISA) coupled with flow injection analysis (FIA) technique provides a very high sensitivity with high throughput of analyses. Automation of this analysis scheme ensures precise detection with high accuracy. The present development aims at providing a user-friendly system for achieving this objective. It employs a 8952 microcontroller for precise flow of reagents, samples, substrate and conjugates used for analysis to be passed through an immobilized antibody column at predetermined time. With the sequence and flow control of buffers used, it also provides the option for reuse of the immobilized antibody column. The system is flexible to accommodate multiple sequences up to a maximum of 99 steps. It is customizable for different flow ELISA applications. It can control up to eight solenoid valves (dc 24 V) and two peristaltic pumps and has one 12 bit analog channel for data acquisition. With the serial interface port, the system provides convenient means for data acquisition into the computer. The system has been successfully tested for immuno analysis of organophosphorous pesticide methyl parathion. (c) 2005 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
microcontroller, methyl parathion, immunosensor, ELISA, flow ELISA
in
Analytica Chimica Acta
volume
560
issue
1-2
pages
30 - 34
publisher
Elsevier
external identifiers
  • wos:000235927500003
  • scopus:33344467408
ISSN
1873-4324
DOI
10.1016/j.aca.2005.12.026
language
English
LU publication?
yes
id
93c9326d-000a-40cf-8914-d8ebe620c4c5 (old id 416591)
date added to LUP
2016-04-01 16:04:43
date last changed
2020-12-29 01:09:18
@article{93c9326d-000a-40cf-8914-d8ebe620c4c5,
  abstract     = {sensitive detection of pesticides is of utmost importance in environment pesticides in agricultural and environmental samples wherein antibodies are employed against the target molecules. Accurate diagnosis depends on the affinity and specificity of the antibody preparation used, and high affinity antibodies are essential for the detection of very small amounts of pesticides. Enzyme linked immuno sorbent assay (ELISA) coupled with flow injection analysis (FIA) technique provides a very high sensitivity with high throughput of analyses. Automation of this analysis scheme ensures precise detection with high accuracy. The present development aims at providing a user-friendly system for achieving this objective. It employs a 8952 microcontroller for precise flow of reagents, samples, substrate and conjugates used for analysis to be passed through an immobilized antibody column at predetermined time. With the sequence and flow control of buffers used, it also provides the option for reuse of the immobilized antibody column. The system is flexible to accommodate multiple sequences up to a maximum of 99 steps. It is customizable for different flow ELISA applications. It can control up to eight solenoid valves (dc 24 V) and two peristaltic pumps and has one 12 bit analog channel for data acquisition. With the serial interface port, the system provides convenient means for data acquisition into the computer. The system has been successfully tested for immuno analysis of organophosphorous pesticide methyl parathion. (c) 2005 Elsevier B.V. All rights reserved.},
  author       = {Kumar, MA and Chouhan, RS and Thakur, MS and Rani, BEA and Mattiasson, Bo and Karanth, NG},
  issn         = {1873-4324},
  language     = {eng},
  number       = {1-2},
  pages        = {30--34},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {Automated flow enzyme-linked immunosorbent assay (ELISA) system for analysis of methyl parathion},
  url          = {http://dx.doi.org/10.1016/j.aca.2005.12.026},
  doi          = {10.1016/j.aca.2005.12.026},
  volume       = {560},
  year         = {2006},
}