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A reusable capacitive immunosensor for carcinoembryonic antigen (CEA) detection using thiourea modified gold electrode

Limbut, W ; Kanatharana, P ; Mattiasson, Bo LU ; Asawatreratanakul, P and Thavarungkul, P (2006) In Analytica Chimica Acta 561(1-2). p.55-61
Abstract
A capacitive immunosensor based on a self-assembled monolayer (SAM) of thiourea on gold electrode has been developed. Anti-carcinoembryonic antigen (anti-CEA) was immobilized on a self-assembled thiourea monolayer (SATUM) via covalent coupling. Under optimum conditions, the decrease in capacitive signal when carcinoembryonic antigen (CEA) standard was injected could be determined with a detection limit of 10 pg ml(-1) and linearity in the range of 0.01-10 ng ml(-1). The immobilized anti-CEA on SATUM gold electrode was stable and after regeneration good reproducibility of the signal could be obtained tip to 45 times with an RSD lower than 3.4%. Good agreement was obtained when CEA concentrations of human serum samples determined by the flow... (More)
A capacitive immunosensor based on a self-assembled monolayer (SAM) of thiourea on gold electrode has been developed. Anti-carcinoembryonic antigen (anti-CEA) was immobilized on a self-assembled thiourea monolayer (SATUM) via covalent coupling. Under optimum conditions, the decrease in capacitive signal when carcinoembryonic antigen (CEA) standard was injected could be determined with a detection limit of 10 pg ml(-1) and linearity in the range of 0.01-10 ng ml(-1). The immobilized anti-CEA on SATUM gold electrode was stable and after regeneration good reproducibility of the signal could be obtained tip to 45 times with an RSD lower than 3.4%. Good agreement was obtained when CEA concentrations of human serum samples determined by the flow injection capacitive immunosensor system were compared to those obtained using an enzyme linked fluorescent assay (ELFA) method (P < 0.05). (c) 2006 Elsevier B.V. All rights reserved. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
carcinoembryonic antigen, capacitance, immunosensor, thiourea, self-assembled monolayer
in
Analytica Chimica Acta
volume
561
issue
1-2
pages
55 - 61
publisher
Elsevier
external identifiers
  • wos:000235945200007
  • scopus:33644819949
ISSN
1873-4324
DOI
10.1016/j.aca.2006.01.021
language
English
LU publication?
yes
id
78e6e07c-8fbe-4983-bb64-9c4dd17f7781 (old id 416609)
date added to LUP
2016-04-01 16:57:42
date last changed
2020-11-10 02:12:21
@article{78e6e07c-8fbe-4983-bb64-9c4dd17f7781,
  abstract     = {A capacitive immunosensor based on a self-assembled monolayer (SAM) of thiourea on gold electrode has been developed. Anti-carcinoembryonic antigen (anti-CEA) was immobilized on a self-assembled thiourea monolayer (SATUM) via covalent coupling. Under optimum conditions, the decrease in capacitive signal when carcinoembryonic antigen (CEA) standard was injected could be determined with a detection limit of 10 pg ml(-1) and linearity in the range of 0.01-10 ng ml(-1). The immobilized anti-CEA on SATUM gold electrode was stable and after regeneration good reproducibility of the signal could be obtained tip to 45 times with an RSD lower than 3.4%. Good agreement was obtained when CEA concentrations of human serum samples determined by the flow injection capacitive immunosensor system were compared to those obtained using an enzyme linked fluorescent assay (ELFA) method (P &lt; 0.05). (c) 2006 Elsevier B.V. All rights reserved.},
  author       = {Limbut, W and Kanatharana, P and Mattiasson, Bo and Asawatreratanakul, P and Thavarungkul, P},
  issn         = {1873-4324},
  language     = {eng},
  number       = {1-2},
  pages        = {55--61},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {A reusable capacitive immunosensor for carcinoembryonic antigen (CEA) detection using thiourea modified gold electrode},
  url          = {http://dx.doi.org/10.1016/j.aca.2006.01.021},
  doi          = {10.1016/j.aca.2006.01.021},
  volume       = {561},
  year         = {2006},
}