A reusable capacitive immunosensor for carcinoembryonic antigen (CEA) detection using thiourea modified gold electrode
(2006) In Analytica Chimica Acta 561(1-2). p.55-61- Abstract
- A capacitive immunosensor based on a self-assembled monolayer (SAM) of thiourea on gold electrode has been developed. Anti-carcinoembryonic antigen (anti-CEA) was immobilized on a self-assembled thiourea monolayer (SATUM) via covalent coupling. Under optimum conditions, the decrease in capacitive signal when carcinoembryonic antigen (CEA) standard was injected could be determined with a detection limit of 10 pg ml(-1) and linearity in the range of 0.01-10 ng ml(-1). The immobilized anti-CEA on SATUM gold electrode was stable and after regeneration good reproducibility of the signal could be obtained tip to 45 times with an RSD lower than 3.4%. Good agreement was obtained when CEA concentrations of human serum samples determined by the flow... (More)
- A capacitive immunosensor based on a self-assembled monolayer (SAM) of thiourea on gold electrode has been developed. Anti-carcinoembryonic antigen (anti-CEA) was immobilized on a self-assembled thiourea monolayer (SATUM) via covalent coupling. Under optimum conditions, the decrease in capacitive signal when carcinoembryonic antigen (CEA) standard was injected could be determined with a detection limit of 10 pg ml(-1) and linearity in the range of 0.01-10 ng ml(-1). The immobilized anti-CEA on SATUM gold electrode was stable and after regeneration good reproducibility of the signal could be obtained tip to 45 times with an RSD lower than 3.4%. Good agreement was obtained when CEA concentrations of human serum samples determined by the flow injection capacitive immunosensor system were compared to those obtained using an enzyme linked fluorescent assay (ELFA) method (P < 0.05). (c) 2006 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/416609
- author
- Limbut, W ; Kanatharana, P ; Mattiasson, Bo LU ; Asawatreratanakul, P and Thavarungkul, P
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- carcinoembryonic antigen, capacitance, immunosensor, thiourea, self-assembled monolayer
- in
- Analytica Chimica Acta
- volume
- 561
- issue
- 1-2
- pages
- 55 - 61
- publisher
- Elsevier
- external identifiers
-
- wos:000235945200007
- scopus:33644819949
- ISSN
- 1873-4324
- DOI
- 10.1016/j.aca.2006.01.021
- language
- English
- LU publication?
- yes
- id
- 78e6e07c-8fbe-4983-bb64-9c4dd17f7781 (old id 416609)
- date added to LUP
- 2016-04-01 16:57:42
- date last changed
- 2022-02-20 17:32:35
@article{78e6e07c-8fbe-4983-bb64-9c4dd17f7781, abstract = {{A capacitive immunosensor based on a self-assembled monolayer (SAM) of thiourea on gold electrode has been developed. Anti-carcinoembryonic antigen (anti-CEA) was immobilized on a self-assembled thiourea monolayer (SATUM) via covalent coupling. Under optimum conditions, the decrease in capacitive signal when carcinoembryonic antigen (CEA) standard was injected could be determined with a detection limit of 10 pg ml(-1) and linearity in the range of 0.01-10 ng ml(-1). The immobilized anti-CEA on SATUM gold electrode was stable and after regeneration good reproducibility of the signal could be obtained tip to 45 times with an RSD lower than 3.4%. Good agreement was obtained when CEA concentrations of human serum samples determined by the flow injection capacitive immunosensor system were compared to those obtained using an enzyme linked fluorescent assay (ELFA) method (P < 0.05). (c) 2006 Elsevier B.V. All rights reserved.}}, author = {{Limbut, W and Kanatharana, P and Mattiasson, Bo and Asawatreratanakul, P and Thavarungkul, P}}, issn = {{1873-4324}}, keywords = {{carcinoembryonic antigen; capacitance; immunosensor; thiourea; self-assembled monolayer}}, language = {{eng}}, number = {{1-2}}, pages = {{55--61}}, publisher = {{Elsevier}}, series = {{Analytica Chimica Acta}}, title = {{A reusable capacitive immunosensor for carcinoembryonic antigen (CEA) detection using thiourea modified gold electrode}}, url = {{http://dx.doi.org/10.1016/j.aca.2006.01.021}}, doi = {{10.1016/j.aca.2006.01.021}}, volume = {{561}}, year = {{2006}}, }