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A novel procedure for the isolation of glycolipids from Bifidobacterium adolescentis 94 BIM using supercritical carbon dioxide

Novik, Galina ; Gamian, A ; da Cruz Francisco, José and Dey, Estera (2006) In Journal of Biotechnology 121(4). p.555-562
Abstract
This study describes a novel isolation procedure for major glycolipids from Bifidobacterium adolescentis 94 BIM. The procedure consists of the use of supercritical carbon dioxide (scCO(2)) with hydro-methanolic solution as co-solvent. The major Glycolipids were isolated using the following operating conditions: pressure, 30 MPa, co-solvent concentration, 10% (9:1, methanol/water, v/v), CO2 flow rate, 5 g/min, extraction time and temperature, 2 h and 55 degrees C, respectively. The reference glycolipids sample was prepared by classical organic solvent extraction followed by chromatographic purification. All isolates were characterized by TLC and the major glycolipids additionally by enzyme linked immunosorbent (ELISA). Sixty milligrams of... (More)
This study describes a novel isolation procedure for major glycolipids from Bifidobacterium adolescentis 94 BIM. The procedure consists of the use of supercritical carbon dioxide (scCO(2)) with hydro-methanolic solution as co-solvent. The major Glycolipids were isolated using the following operating conditions: pressure, 30 MPa, co-solvent concentration, 10% (9:1, methanol/water, v/v), CO2 flow rate, 5 g/min, extraction time and temperature, 2 h and 55 degrees C, respectively. The reference glycolipids sample was prepared by classical organic solvent extraction followed by chromatographic purification. All isolates were characterized by TLC and the major glycolipids additionally by enzyme linked immunosorbent (ELISA). Sixty milligrams of glycolipids with similar immunoreactivity as the reference glycolipids were isolated from I a of freeze-dried biomass (6% of yield). (c) 2005 Elsevier B.V. All rights reserved. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
immunosorbent assay (ELISA), enzyme linked, Bifidobacterium adolescentis, bifidobacteria, neutral lipids, supercritical carbon dioxide, glycolipids
in
Journal of Biotechnology
volume
121
issue
4
pages
555 - 562
publisher
Elsevier
external identifiers
  • pmid:16229918
  • wos:000235697100013
  • scopus:32144462464
ISSN
1873-4863
DOI
10.1016/j.jbiotec.2005.08.018
language
English
LU publication?
yes
id
145f1290-a125-4aeb-bbff-0c53285f2792 (old id 416739)
date added to LUP
2016-04-01 11:42:47
date last changed
2021-08-11 02:35:49
@article{145f1290-a125-4aeb-bbff-0c53285f2792,
  abstract     = {This study describes a novel isolation procedure for major glycolipids from Bifidobacterium adolescentis 94 BIM. The procedure consists of the use of supercritical carbon dioxide (scCO(2)) with hydro-methanolic solution as co-solvent. The major Glycolipids were isolated using the following operating conditions: pressure, 30 MPa, co-solvent concentration, 10% (9:1, methanol/water, v/v), CO2 flow rate, 5 g/min, extraction time and temperature, 2 h and 55 degrees C, respectively. The reference glycolipids sample was prepared by classical organic solvent extraction followed by chromatographic purification. All isolates were characterized by TLC and the major glycolipids additionally by enzyme linked immunosorbent (ELISA). Sixty milligrams of glycolipids with similar immunoreactivity as the reference glycolipids were isolated from I a of freeze-dried biomass (6% of yield). (c) 2005 Elsevier B.V. All rights reserved.},
  author       = {Novik, Galina and Gamian, A and da Cruz Francisco, José and Dey, Estera},
  issn         = {1873-4863},
  language     = {eng},
  number       = {4},
  pages        = {555--562},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {A novel procedure for the isolation of glycolipids from Bifidobacterium adolescentis 94 BIM using supercritical carbon dioxide},
  url          = {http://dx.doi.org/10.1016/j.jbiotec.2005.08.018},
  doi          = {10.1016/j.jbiotec.2005.08.018},
  volume       = {121},
  year         = {2006},
}