A novel procedure for the isolation of glycolipids from Bifidobacterium adolescentis 94 BIM using supercritical carbon dioxide
(2006) In Journal of Biotechnology 121(4). p.555-562- Abstract
- This study describes a novel isolation procedure for major glycolipids from Bifidobacterium adolescentis 94 BIM. The procedure consists of the use of supercritical carbon dioxide (scCO(2)) with hydro-methanolic solution as co-solvent. The major Glycolipids were isolated using the following operating conditions: pressure, 30 MPa, co-solvent concentration, 10% (9:1, methanol/water, v/v), CO2 flow rate, 5 g/min, extraction time and temperature, 2 h and 55 degrees C, respectively. The reference glycolipids sample was prepared by classical organic solvent extraction followed by chromatographic purification. All isolates were characterized by TLC and the major glycolipids additionally by enzyme linked immunosorbent (ELISA). Sixty milligrams of... (More)
- This study describes a novel isolation procedure for major glycolipids from Bifidobacterium adolescentis 94 BIM. The procedure consists of the use of supercritical carbon dioxide (scCO(2)) with hydro-methanolic solution as co-solvent. The major Glycolipids were isolated using the following operating conditions: pressure, 30 MPa, co-solvent concentration, 10% (9:1, methanol/water, v/v), CO2 flow rate, 5 g/min, extraction time and temperature, 2 h and 55 degrees C, respectively. The reference glycolipids sample was prepared by classical organic solvent extraction followed by chromatographic purification. All isolates were characterized by TLC and the major glycolipids additionally by enzyme linked immunosorbent (ELISA). Sixty milligrams of glycolipids with similar immunoreactivity as the reference glycolipids were isolated from I a of freeze-dried biomass (6% of yield). (c) 2005 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/416739
- author
- Novik, Galina LU ; Gamian, A ; da Cruz Francisco, José LU and Dey, Estera LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- immunosorbent assay (ELISA), enzyme linked, Bifidobacterium adolescentis, bifidobacteria, neutral lipids, supercritical carbon dioxide, glycolipids
- in
- Journal of Biotechnology
- volume
- 121
- issue
- 4
- pages
- 555 - 562
- publisher
- Elsevier
- external identifiers
-
- pmid:16229918
- wos:000235697100013
- scopus:32144462464
- ISSN
- 1873-4863
- DOI
- 10.1016/j.jbiotec.2005.08.018
- language
- English
- LU publication?
- yes
- id
- 145f1290-a125-4aeb-bbff-0c53285f2792 (old id 416739)
- date added to LUP
- 2016-04-01 11:42:47
- date last changed
- 2022-01-26 17:06:31
@article{145f1290-a125-4aeb-bbff-0c53285f2792, abstract = {{This study describes a novel isolation procedure for major glycolipids from Bifidobacterium adolescentis 94 BIM. The procedure consists of the use of supercritical carbon dioxide (scCO(2)) with hydro-methanolic solution as co-solvent. The major Glycolipids were isolated using the following operating conditions: pressure, 30 MPa, co-solvent concentration, 10% (9:1, methanol/water, v/v), CO2 flow rate, 5 g/min, extraction time and temperature, 2 h and 55 degrees C, respectively. The reference glycolipids sample was prepared by classical organic solvent extraction followed by chromatographic purification. All isolates were characterized by TLC and the major glycolipids additionally by enzyme linked immunosorbent (ELISA). Sixty milligrams of glycolipids with similar immunoreactivity as the reference glycolipids were isolated from I a of freeze-dried biomass (6% of yield). (c) 2005 Elsevier B.V. All rights reserved.}}, author = {{Novik, Galina and Gamian, A and da Cruz Francisco, José and Dey, Estera}}, issn = {{1873-4863}}, keywords = {{immunosorbent assay (ELISA); enzyme linked; Bifidobacterium adolescentis; bifidobacteria; neutral lipids; supercritical carbon dioxide; glycolipids}}, language = {{eng}}, number = {{4}}, pages = {{555--562}}, publisher = {{Elsevier}}, series = {{Journal of Biotechnology}}, title = {{A novel procedure for the isolation of glycolipids from Bifidobacterium adolescentis 94 BIM using supercritical carbon dioxide}}, url = {{http://dx.doi.org/10.1016/j.jbiotec.2005.08.018}}, doi = {{10.1016/j.jbiotec.2005.08.018}}, volume = {{121}}, year = {{2006}}, }