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Levels of cysteinyl-leukotrienes in exhaled breath condensate are not due to saliva contamination

Tufvesson, Ellen LU ; van Weele, Linda Jacqueline ; Ekedahl, Henrik LU and Bjermer, Leif LU (2010) In Clinical Respiratory Journal 4(2). p.83-88
Abstract
Background and Aims: Saliva contamination has been suggested to be a major contributor to levels of cysteinyl leukotrienes in exhaled breath condensate (EBC). The aim of this study was to compare the levels of cysteinyl-leukotrienes (CysLT) and alpha-amylase activity in EBC to induced sputum and saliva collected from the same subjects (asthmatics and control). We thereby aimed to find out whether saliva contamination could be a plausible explanation to the levels found in EBC or not. Methods: EBC, saliva and induced sputum were collected from 11 asthmatic and 19 healthy adults. These samples were analyzed for CysLT concentration and alpha-amylase activity. Results: No significant correlation was found between CysLT concentration and... (More)
Background and Aims: Saliva contamination has been suggested to be a major contributor to levels of cysteinyl leukotrienes in exhaled breath condensate (EBC). The aim of this study was to compare the levels of cysteinyl-leukotrienes (CysLT) and alpha-amylase activity in EBC to induced sputum and saliva collected from the same subjects (asthmatics and control). We thereby aimed to find out whether saliva contamination could be a plausible explanation to the levels found in EBC or not. Methods: EBC, saliva and induced sputum were collected from 11 asthmatic and 19 healthy adults. These samples were analyzed for CysLT concentration and alpha-amylase activity. Results: No significant correlation was found between CysLT concentration and alpha-amylase activity in EBC, saliva or sputum. In addition, we show that the saliva contamination (measured as alpha-amylase activity) was negligible, as the relative amount of saliva CysLT was only 0.6% of that found in EBC. The amount of CysLT correlated between all three compartments (EBC, saliva and sputum), but no similar correlation was seen for the alpha-amylase activity in EBC compared to saliva and sputum. The levels of CysLT were higher in asthmatic patients compared to healthy controls in EBC, saliva and sputum. Conclusion: We conclude that the amount of CysLT in EBC cannot be explained by saliva contamination. Please cite this paper as: Tufvesson E, van Weele LJ, Ekedahl H and Bjermer L. Levels of cysteinyl-leukotrienes in exhaled breath condensate are not due to saliva contamination. The Clinical Respiratory Journal 2010; 4: 83-88. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
exhaled breath, cysteinyl-leukotrienes, alpha-amylase, asthma, saliva, sputum, condensate
in
Clinical Respiratory Journal
volume
4
issue
2
pages
83 - 88
publisher
Wiley-Blackwell
external identifiers
  • wos:000275766600004
  • pmid:20565481
  • scopus:77949881057
  • pmid:20565481
ISSN
1752-6981
DOI
10.1111/j.1752-699X.2009.00151.x
language
English
LU publication?
yes
id
416d493d-c08e-4c43-923d-e32205681944 (old id 1588105)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20565481?dopt=Abstract
date added to LUP
2016-04-01 10:38:22
date last changed
2022-01-26 01:07:41
@article{416d493d-c08e-4c43-923d-e32205681944,
  abstract     = {{Background and Aims: Saliva contamination has been suggested to be a major contributor to levels of cysteinyl leukotrienes in exhaled breath condensate (EBC). The aim of this study was to compare the levels of cysteinyl-leukotrienes (CysLT) and alpha-amylase activity in EBC to induced sputum and saliva collected from the same subjects (asthmatics and control). We thereby aimed to find out whether saliva contamination could be a plausible explanation to the levels found in EBC or not. Methods: EBC, saliva and induced sputum were collected from 11 asthmatic and 19 healthy adults. These samples were analyzed for CysLT concentration and alpha-amylase activity. Results: No significant correlation was found between CysLT concentration and alpha-amylase activity in EBC, saliva or sputum. In addition, we show that the saliva contamination (measured as alpha-amylase activity) was negligible, as the relative amount of saliva CysLT was only 0.6% of that found in EBC. The amount of CysLT correlated between all three compartments (EBC, saliva and sputum), but no similar correlation was seen for the alpha-amylase activity in EBC compared to saliva and sputum. The levels of CysLT were higher in asthmatic patients compared to healthy controls in EBC, saliva and sputum. Conclusion: We conclude that the amount of CysLT in EBC cannot be explained by saliva contamination. Please cite this paper as: Tufvesson E, van Weele LJ, Ekedahl H and Bjermer L. Levels of cysteinyl-leukotrienes in exhaled breath condensate are not due to saliva contamination. The Clinical Respiratory Journal 2010; 4: 83-88.}},
  author       = {{Tufvesson, Ellen and van Weele, Linda Jacqueline and Ekedahl, Henrik and Bjermer, Leif}},
  issn         = {{1752-6981}},
  keywords     = {{exhaled breath; cysteinyl-leukotrienes; alpha-amylase; asthma; saliva; sputum; condensate}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{83--88}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Clinical Respiratory Journal}},
  title        = {{Levels of cysteinyl-leukotrienes in exhaled breath condensate are not due to saliva contamination}},
  url          = {{http://dx.doi.org/10.1111/j.1752-699X.2009.00151.x}},
  doi          = {{10.1111/j.1752-699X.2009.00151.x}},
  volume       = {{4}},
  year         = {{2010}},
}