Simulated moving bed technology with a simplified approach for protein purification - Separation of lactoperoxidase and lactoferrin from whey protein concentrate
(2006) In Journal of Chromatography A 1107(1-2). p.88-95- Abstract
- Simulated moving bed (SMB) technology is a continuous chromatographic technique proven to have many advantages compared to conventional batch chromatography, such as: raised productivity and product concentration, reduced buffer consumption as well as more efficient use of raw material. In this study a 20 column SMB process for the separation of lactoperoxidase and lactoferrin from whey protein concentrate (WPC) was developed. A simplified approach with data from a single column experiment was used when designing the process. The SMB process data were compared to a theoretical scale-up of the breakthrough experiment reflecting the same 20 column set-up run in non-moving bed mode. The outcome of the comparison is a 48% raise in... (More)
- Simulated moving bed (SMB) technology is a continuous chromatographic technique proven to have many advantages compared to conventional batch chromatography, such as: raised productivity and product concentration, reduced buffer consumption as well as more efficient use of raw material. In this study a 20 column SMB process for the separation of lactoperoxidase and lactoferrin from whey protein concentrate (WPC) was developed. A simplified approach with data from a single column experiment was used when designing the process. The SMB process data were compared to a theoretical scale-up of the breakthrough experiment reflecting the same 20 column set-up run in non-moving bed mode. The outcome of the comparison is a 48% raise in productivity, a 4.3 times decrease in buffer consumption, 6.5 times raise in target protein concentration with a raw material utilization which is slightly better for the SMB process. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/417121
- author
- Andersson, Jonatan LU and Mattiasson, Bo LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cation exchange, whey, WPC, lactoferrin, lactoperoxidase, separation, protein, simulated moving bed, SMB
- in
- Journal of Chromatography A
- volume
- 1107
- issue
- 1-2
- pages
- 88 - 95
- publisher
- Elsevier
- external identifiers
-
- pmid:16387313
- wos:000235505100011
- scopus:31844449572
- ISSN
- 0021-9673
- DOI
- 10.1016/j.chroma.2005.12.018
- language
- English
- LU publication?
- yes
- id
- 5de30997-4e99-49a4-8f88-5d7263fd8d18 (old id 417121)
- date added to LUP
- 2016-04-01 16:16:24
- date last changed
- 2022-03-14 23:13:48
@article{5de30997-4e99-49a4-8f88-5d7263fd8d18, abstract = {{Simulated moving bed (SMB) technology is a continuous chromatographic technique proven to have many advantages compared to conventional batch chromatography, such as: raised productivity and product concentration, reduced buffer consumption as well as more efficient use of raw material. In this study a 20 column SMB process for the separation of lactoperoxidase and lactoferrin from whey protein concentrate (WPC) was developed. A simplified approach with data from a single column experiment was used when designing the process. The SMB process data were compared to a theoretical scale-up of the breakthrough experiment reflecting the same 20 column set-up run in non-moving bed mode. The outcome of the comparison is a 48% raise in productivity, a 4.3 times decrease in buffer consumption, 6.5 times raise in target protein concentration with a raw material utilization which is slightly better for the SMB process.}}, author = {{Andersson, Jonatan and Mattiasson, Bo}}, issn = {{0021-9673}}, keywords = {{cation exchange; whey; WPC; lactoferrin; lactoperoxidase; separation; protein; simulated moving bed; SMB}}, language = {{eng}}, number = {{1-2}}, pages = {{88--95}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography A}}, title = {{Simulated moving bed technology with a simplified approach for protein purification - Separation of lactoperoxidase and lactoferrin from whey protein concentrate}}, url = {{http://dx.doi.org/10.1016/j.chroma.2005.12.018}}, doi = {{10.1016/j.chroma.2005.12.018}}, volume = {{1107}}, year = {{2006}}, }