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Simulated moving bed technology with a simplified approach for protein purification - Separation of lactoperoxidase and lactoferrin from whey protein concentrate

Andersson, Jonatan LU and Mattiasson, Bo LU (2006) In Journal of Chromatography A 1107(1-2). p.88-95
Abstract
Simulated moving bed (SMB) technology is a continuous chromatographic technique proven to have many advantages compared to conventional batch chromatography, such as: raised productivity and product concentration, reduced buffer consumption as well as more efficient use of raw material. In this study a 20 column SMB process for the separation of lactoperoxidase and lactoferrin from whey protein concentrate (WPC) was developed. A simplified approach with data from a single column experiment was used when designing the process. The SMB process data were compared to a theoretical scale-up of the breakthrough experiment reflecting the same 20 column set-up run in non-moving bed mode. The outcome of the comparison is a 48% raise in... (More)
Simulated moving bed (SMB) technology is a continuous chromatographic technique proven to have many advantages compared to conventional batch chromatography, such as: raised productivity and product concentration, reduced buffer consumption as well as more efficient use of raw material. In this study a 20 column SMB process for the separation of lactoperoxidase and lactoferrin from whey protein concentrate (WPC) was developed. A simplified approach with data from a single column experiment was used when designing the process. The SMB process data were compared to a theoretical scale-up of the breakthrough experiment reflecting the same 20 column set-up run in non-moving bed mode. The outcome of the comparison is a 48% raise in productivity, a 4.3 times decrease in buffer consumption, 6.5 times raise in target protein concentration with a raw material utilization which is slightly better for the SMB process. (Less)
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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cation exchange, whey, WPC, lactoferrin, lactoperoxidase, separation, protein, simulated moving bed, SMB
in
Journal of Chromatography A
volume
1107
issue
1-2
pages
88 - 95
publisher
Elsevier
external identifiers
  • pmid:16387313
  • wos:000235505100011
  • scopus:31844449572
ISSN
0021-9673
DOI
10.1016/j.chroma.2005.12.018
language
English
LU publication?
yes
id
5de30997-4e99-49a4-8f88-5d7263fd8d18 (old id 417121)
date added to LUP
2016-04-01 16:16:24
date last changed
2021-05-25 04:19:55
@article{5de30997-4e99-49a4-8f88-5d7263fd8d18,
  abstract     = {Simulated moving bed (SMB) technology is a continuous chromatographic technique proven to have many advantages compared to conventional batch chromatography, such as: raised productivity and product concentration, reduced buffer consumption as well as more efficient use of raw material. In this study a 20 column SMB process for the separation of lactoperoxidase and lactoferrin from whey protein concentrate (WPC) was developed. A simplified approach with data from a single column experiment was used when designing the process. The SMB process data were compared to a theoretical scale-up of the breakthrough experiment reflecting the same 20 column set-up run in non-moving bed mode. The outcome of the comparison is a 48% raise in productivity, a 4.3 times decrease in buffer consumption, 6.5 times raise in target protein concentration with a raw material utilization which is slightly better for the SMB process.},
  author       = {Andersson, Jonatan and Mattiasson, Bo},
  issn         = {0021-9673},
  language     = {eng},
  number       = {1-2},
  pages        = {88--95},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Simulated moving bed technology with a simplified approach for protein purification - Separation of lactoperoxidase and lactoferrin from whey protein concentrate},
  url          = {http://dx.doi.org/10.1016/j.chroma.2005.12.018},
  doi          = {10.1016/j.chroma.2005.12.018},
  volume       = {1107},
  year         = {2006},
}