Rapid one-step whole blood C-reactive protein magnetic permeability immunoassay with monoclonal antibody conjugated nanoparticles as superparamagnetic labels and enhanced sedimentation
(2006) In Analytical and Bioanalytical Chemistry 384(3). p.651-657- Abstract
- A rapid (5.5 min) one-step whole blood C-reactive protein (CRP) magnetic permeability immunoassay utilizing monoclonal antibody conjugated dextran iron oxide nanoparticles (70 nm) as superparamagnetic labels and mixed fractions (1:1 ratio of 15-40 and 60 mu m) of polyclonal anti-CRP conjugated silica microparticles for enhanced sedimentation is described. In this one-step assay procedure, a whole blood sample (4 mu l) is applied to an assay glass vial, containing both antibody conjugates, and mixed for 30 s. The target analyte, CRP, forms a sandwich complex between the conjugated nanoparticles and microparticles, and, subsequently, the complex sediments under normal gravitation within 5 min to the bottom of the vial. The magnetic... (More)
- A rapid (5.5 min) one-step whole blood C-reactive protein (CRP) magnetic permeability immunoassay utilizing monoclonal antibody conjugated dextran iron oxide nanoparticles (70 nm) as superparamagnetic labels and mixed fractions (1:1 ratio of 15-40 and 60 mu m) of polyclonal anti-CRP conjugated silica microparticles for enhanced sedimentation is described. In this one-step assay procedure, a whole blood sample (4 mu l) is applied to an assay glass vial, containing both antibody conjugates, and mixed for 30 s. The target analyte, CRP, forms a sandwich complex between the conjugated nanoparticles and microparticles, and, subsequently, the complex sediments under normal gravitation within 5 min to the bottom of the vial. The magnetic permeability increase of the sediment due to the presence of the complexed superparamagnetic nanoparticles is determined using an inductance-based transducer. Assayed patient whole blood samples were compared with the Abbott Diagnostics Architect reference method. A strong linear correlation was observed for the CRP concentration range 0-260 mg/l in whole blood (y=1.001x+0.42, R-2=0.982, n=50). The CRP assay presented showed a limit of detection of 3 mg/l and a total imprecision (coefficient of variation) of 10.5%. On the basis of our observations, we propose a rapid, one-step, CRP assay for near-patient testing. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/417646
- author
- Ibraimi, Filiz LU ; Kriz, D ; Lu, M ; Hansson, LO and Kriz, Kirstin LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- immunoassay, magneto, magnetic permeability, superparamagnetic nanoparticles, C-reactive protein, point-of-care
- in
- Analytical and Bioanalytical Chemistry
- volume
- 384
- issue
- 3
- pages
- 651 - 657
- publisher
- Springer
- external identifiers
-
- wos:000235339600012
- scopus:32544447073
- ISSN
- 1618-2642
- DOI
- 10.1007/s00216-005-0094-6
- language
- English
- LU publication?
- yes
- id
- bbb8806b-a252-444a-a43d-0278181675a7 (old id 417646)
- date added to LUP
- 2016-04-01 12:35:10
- date last changed
- 2022-04-13 21:01:01
@article{bbb8806b-a252-444a-a43d-0278181675a7,
abstract = {{A rapid (5.5 min) one-step whole blood C-reactive protein (CRP) magnetic permeability immunoassay utilizing monoclonal antibody conjugated dextran iron oxide nanoparticles (70 nm) as superparamagnetic labels and mixed fractions (1:1 ratio of 15-40 and 60 mu m) of polyclonal anti-CRP conjugated silica microparticles for enhanced sedimentation is described. In this one-step assay procedure, a whole blood sample (4 mu l) is applied to an assay glass vial, containing both antibody conjugates, and mixed for 30 s. The target analyte, CRP, forms a sandwich complex between the conjugated nanoparticles and microparticles, and, subsequently, the complex sediments under normal gravitation within 5 min to the bottom of the vial. The magnetic permeability increase of the sediment due to the presence of the complexed superparamagnetic nanoparticles is determined using an inductance-based transducer. Assayed patient whole blood samples were compared with the Abbott Diagnostics Architect reference method. A strong linear correlation was observed for the CRP concentration range 0-260 mg/l in whole blood (y=1.001x+0.42, R-2=0.982, n=50). The CRP assay presented showed a limit of detection of 3 mg/l and a total imprecision (coefficient of variation) of 10.5%. On the basis of our observations, we propose a rapid, one-step, CRP assay for near-patient testing.}},
author = {{Ibraimi, Filiz and Kriz, D and Lu, M and Hansson, LO and Kriz, Kirstin}},
issn = {{1618-2642}},
keywords = {{immunoassay; magneto; magnetic permeability; superparamagnetic nanoparticles; C-reactive protein; point-of-care}},
language = {{eng}},
number = {{3}},
pages = {{651--657}},
publisher = {{Springer}},
series = {{Analytical and Bioanalytical Chemistry}},
title = {{Rapid one-step whole blood C-reactive protein magnetic permeability immunoassay with monoclonal antibody conjugated nanoparticles as superparamagnetic labels and enhanced sedimentation}},
url = {{http://dx.doi.org/10.1007/s00216-005-0094-6}},
doi = {{10.1007/s00216-005-0094-6}},
volume = {{384}},
year = {{2006}},
}