Rapid fluorometric screening of antibiotics in seafood
(2006) In Microchimica Acta 153(3-4). p.133-137- Abstract
- Simple and rapid fluorometric screening methods have been developed based on the competitive binding between the target and an intercalating fluorophore dye to double-stranded-DNA (dsDNA). In this study, the long-wavelength fluorescente dye TOTO-3 was employed as the indicator. Compounds that interact with dsDNA will affect the binding of TOTO-3 to the nucleic acid thereby changing the fluorescence intensity. The analyte concentration is indirectly determined by the decrease in fluorescence intensity. A fiber optic fluorescence screening system was developed for rapid and convenient sample processing. Lambda DNA (48.5kb) was chosen as a suitable sensing nucleic acid material. Detection of sulfathiazole and chloramphenicol in shrimps using... (More)
- Simple and rapid fluorometric screening methods have been developed based on the competitive binding between the target and an intercalating fluorophore dye to double-stranded-DNA (dsDNA). In this study, the long-wavelength fluorescente dye TOTO-3 was employed as the indicator. Compounds that interact with dsDNA will affect the binding of TOTO-3 to the nucleic acid thereby changing the fluorescence intensity. The analyte concentration is indirectly determined by the decrease in fluorescence intensity. A fiber optic fluorescence screening system was developed for rapid and convenient sample processing. Lambda DNA (48.5kb) was chosen as a suitable sensing nucleic acid material. Detection of sulfathiazole and chloramphenicol in shrimps using this method was studied in the range of 0.5-25ng mL(-1) of sulfathiazole and of 1-50ng mL(-1) of chloramphenicol. Detection limits of 0.5ng mL(-1) of sulfathiazole and 1ng mL(-1) of chloramphenicol were achieved. This approach is useful as a routine test in the monitoring of antibiotics in the environment or aquaculture products. The easy operation and the rapid and sensitive detection make this a potential high-throughput screening method. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/417688
- author
- Liu, Y and Danielsson, Bengt LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- fluorometric screening, screening, DNA-based drug, sulfathiazole, chloramphenicol, seafood contamination
- in
- Microchimica Acta
- volume
- 153
- issue
- 3-4
- pages
- 133 - 137
- publisher
- Springer
- external identifiers
-
- wos:000235202500003
- scopus:32144432310
- ISSN
- 1436-5073
- DOI
- 10.1007/s00604-005-0421-9
- language
- English
- LU publication?
- yes
- id
- 9ce9b9d6-ffa9-4fd9-93cc-59c411065b3d (old id 417688)
- date added to LUP
- 2016-04-01 12:20:00
- date last changed
- 2023-01-03 07:04:22
@article{9ce9b9d6-ffa9-4fd9-93cc-59c411065b3d, abstract = {{Simple and rapid fluorometric screening methods have been developed based on the competitive binding between the target and an intercalating fluorophore dye to double-stranded-DNA (dsDNA). In this study, the long-wavelength fluorescente dye TOTO-3 was employed as the indicator. Compounds that interact with dsDNA will affect the binding of TOTO-3 to the nucleic acid thereby changing the fluorescence intensity. The analyte concentration is indirectly determined by the decrease in fluorescence intensity. A fiber optic fluorescence screening system was developed for rapid and convenient sample processing. Lambda DNA (48.5kb) was chosen as a suitable sensing nucleic acid material. Detection of sulfathiazole and chloramphenicol in shrimps using this method was studied in the range of 0.5-25ng mL(-1) of sulfathiazole and of 1-50ng mL(-1) of chloramphenicol. Detection limits of 0.5ng mL(-1) of sulfathiazole and 1ng mL(-1) of chloramphenicol were achieved. This approach is useful as a routine test in the monitoring of antibiotics in the environment or aquaculture products. The easy operation and the rapid and sensitive detection make this a potential high-throughput screening method.}}, author = {{Liu, Y and Danielsson, Bengt}}, issn = {{1436-5073}}, keywords = {{fluorometric screening; screening; DNA-based drug; sulfathiazole; chloramphenicol; seafood contamination}}, language = {{eng}}, number = {{3-4}}, pages = {{133--137}}, publisher = {{Springer}}, series = {{Microchimica Acta}}, title = {{Rapid fluorometric screening of antibiotics in seafood}}, url = {{http://dx.doi.org/10.1007/s00604-005-0421-9}}, doi = {{10.1007/s00604-005-0421-9}}, volume = {{153}}, year = {{2006}}, }