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Rapid fluorometric screening of antibiotics in seafood

Liu, Y and Danielsson, Bengt LU (2006) In Microchimica Acta 153(3-4). p.133-137
Abstract
Simple and rapid fluorometric screening methods have been developed based on the competitive binding between the target and an intercalating fluorophore dye to double-stranded-DNA (dsDNA). In this study, the long-wavelength fluorescente dye TOTO-3 was employed as the indicator. Compounds that interact with dsDNA will affect the binding of TOTO-3 to the nucleic acid thereby changing the fluorescence intensity. The analyte concentration is indirectly determined by the decrease in fluorescence intensity. A fiber optic fluorescence screening system was developed for rapid and convenient sample processing. Lambda DNA (48.5kb) was chosen as a suitable sensing nucleic acid material. Detection of sulfathiazole and chloramphenicol in shrimps using... (More)
Simple and rapid fluorometric screening methods have been developed based on the competitive binding between the target and an intercalating fluorophore dye to double-stranded-DNA (dsDNA). In this study, the long-wavelength fluorescente dye TOTO-3 was employed as the indicator. Compounds that interact with dsDNA will affect the binding of TOTO-3 to the nucleic acid thereby changing the fluorescence intensity. The analyte concentration is indirectly determined by the decrease in fluorescence intensity. A fiber optic fluorescence screening system was developed for rapid and convenient sample processing. Lambda DNA (48.5kb) was chosen as a suitable sensing nucleic acid material. Detection of sulfathiazole and chloramphenicol in shrimps using this method was studied in the range of 0.5-25ng mL(-1) of sulfathiazole and of 1-50ng mL(-1) of chloramphenicol. Detection limits of 0.5ng mL(-1) of sulfathiazole and 1ng mL(-1) of chloramphenicol were achieved. This approach is useful as a routine test in the monitoring of antibiotics in the environment or aquaculture products. The easy operation and the rapid and sensitive detection make this a potential high-throughput screening method. (Less)
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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
fluorometric screening, screening, DNA-based drug, sulfathiazole, chloramphenicol, seafood contamination
in
Microchimica Acta
volume
153
issue
3-4
pages
133 - 137
publisher
Springer
external identifiers
  • wos:000235202500003
  • scopus:32144432310
ISSN
1436-5073
DOI
10.1007/s00604-005-0421-9
language
English
LU publication?
yes
id
9ce9b9d6-ffa9-4fd9-93cc-59c411065b3d (old id 417688)
date added to LUP
2016-04-01 12:20:00
date last changed
2025-01-02 14:13:04
@article{9ce9b9d6-ffa9-4fd9-93cc-59c411065b3d,
  abstract     = {{Simple and rapid fluorometric screening methods have been developed based on the competitive binding between the target and an intercalating fluorophore dye to double-stranded-DNA (dsDNA). In this study, the long-wavelength fluorescente dye TOTO-3 was employed as the indicator. Compounds that interact with dsDNA will affect the binding of TOTO-3 to the nucleic acid thereby changing the fluorescence intensity. The analyte concentration is indirectly determined by the decrease in fluorescence intensity. A fiber optic fluorescence screening system was developed for rapid and convenient sample processing. Lambda DNA (48.5kb) was chosen as a suitable sensing nucleic acid material. Detection of sulfathiazole and chloramphenicol in shrimps using this method was studied in the range of 0.5-25ng mL(-1) of sulfathiazole and of 1-50ng mL(-1) of chloramphenicol. Detection limits of 0.5ng mL(-1) of sulfathiazole and 1ng mL(-1) of chloramphenicol were achieved. This approach is useful as a routine test in the monitoring of antibiotics in the environment or aquaculture products. The easy operation and the rapid and sensitive detection make this a potential high-throughput screening method.}},
  author       = {{Liu, Y and Danielsson, Bengt}},
  issn         = {{1436-5073}},
  keywords     = {{fluorometric screening; screening; DNA-based drug; sulfathiazole; chloramphenicol; seafood contamination}},
  language     = {{eng}},
  number       = {{3-4}},
  pages        = {{133--137}},
  publisher    = {{Springer}},
  series       = {{Microchimica Acta}},
  title        = {{Rapid fluorometric screening of antibiotics in seafood}},
  url          = {{http://dx.doi.org/10.1007/s00604-005-0421-9}},
  doi          = {{10.1007/s00604-005-0421-9}},
  volume       = {{153}},
  year         = {{2006}},
}