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Detachment of affinity-captured bioparticles by elastic deformation of a macroporous hydrogel

Dainiak, Maria LU ; Kumar, Ashok LU ; Galaev, Igor LU and Mattiasson, Bo LU (2006) In Proceedings of the National Academy of Sciences 103(4). p.849-854
Abstract
Adsorption of bioparticles to affinity surfaces involves polyvalent interactions, complicating greatly the recovery of the adsorbed material. A unique system for the efficient binding and release of different cells and particles is described. Affinity-bound bioparticles and synthetic particles are detached from the macroporous hydrogel matrix, a so-called cryogel, when the cryogel undergoes elastic deformation. The particle detachment upon elastic deformation is believed to be due to breaking of many of the multipoint attachments between the particles and the affinity matrix and the change in the distance between affinity ligands when the matrix is deformed. However, no release of affinity-bound protein occurred upon elastic deformation.... (More)
Adsorption of bioparticles to affinity surfaces involves polyvalent interactions, complicating greatly the recovery of the adsorbed material. A unique system for the efficient binding and release of different cells and particles is described. Affinity-bound bioparticles and synthetic particles are detached from the macroporous hydrogel matrix, a so-called cryogel, when the cryogel undergoes elastic deformation. The particle detachment upon elastic deformation is believed to be due to breaking of many of the multipoint attachments between the particles and the affinity matrix and the change in the distance between affinity ligands when the matrix is deformed. However, no release of affinity-bound protein occurred upon elastic deformation. The phenomenon of particle detachment upon elastic deformation is believed to be of a generic nature, because it was demonstrated for a variety of bioparticles of different sizes and for synthetic particles, for different ligand-receptor pairs (IgG-protein A, sugar-ConA, metal ion-chelating ligand), and when the deformation was caused by either external forces (mechanical deformation) or internal forces (the shrinkage of thermosensitive, macroporous hydrogel upon an increase in temperature). The elasticity of cryogel monoliths ensures high recovery of captured cells under mild conditions, with highly retained viability. This property, along with their continuous porous structure makes cryogel monoliths very attractive for applications in affinity cell separation. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
thermosensitive hydrogels, cell separation, affinity cryogel monoliths, polyvalent interactions, cell release
in
Proceedings of the National Academy of Sciences
volume
103
issue
4
pages
849 - 854
publisher
National Acad Sciences
external identifiers
  • wos:000234938300005
  • pmid:16418282
  • scopus:32244446815
ISSN
1091-6490
DOI
10.1073/pnas.0508432103
language
English
LU publication?
yes
id
2f3b15e2-06e7-4c76-9593-d73805753903 (old id 418340)
date added to LUP
2016-04-01 12:20:36
date last changed
2021-02-17 03:14:37
@article{2f3b15e2-06e7-4c76-9593-d73805753903,
  abstract     = {Adsorption of bioparticles to affinity surfaces involves polyvalent interactions, complicating greatly the recovery of the adsorbed material. A unique system for the efficient binding and release of different cells and particles is described. Affinity-bound bioparticles and synthetic particles are detached from the macroporous hydrogel matrix, a so-called cryogel, when the cryogel undergoes elastic deformation. The particle detachment upon elastic deformation is believed to be due to breaking of many of the multipoint attachments between the particles and the affinity matrix and the change in the distance between affinity ligands when the matrix is deformed. However, no release of affinity-bound protein occurred upon elastic deformation. The phenomenon of particle detachment upon elastic deformation is believed to be of a generic nature, because it was demonstrated for a variety of bioparticles of different sizes and for synthetic particles, for different ligand-receptor pairs (IgG-protein A, sugar-ConA, metal ion-chelating ligand), and when the deformation was caused by either external forces (mechanical deformation) or internal forces (the shrinkage of thermosensitive, macroporous hydrogel upon an increase in temperature). The elasticity of cryogel monoliths ensures high recovery of captured cells under mild conditions, with highly retained viability. This property, along with their continuous porous structure makes cryogel monoliths very attractive for applications in affinity cell separation.},
  author       = {Dainiak, Maria and Kumar, Ashok and Galaev, Igor and Mattiasson, Bo},
  issn         = {1091-6490},
  language     = {eng},
  number       = {4},
  pages        = {849--854},
  publisher    = {National Acad Sciences},
  series       = {Proceedings of the National Academy of Sciences},
  title        = {Detachment of affinity-captured bioparticles by elastic deformation of a macroporous hydrogel},
  url          = {http://dx.doi.org/10.1073/pnas.0508432103},
  doi          = {10.1073/pnas.0508432103},
  volume       = {103},
  year         = {2006},
}