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Bladder volume alters cholinergic responses of the isolated whole mouse bladder

Lagou, M ; Gillespie, JI ; Andersson, Karl-Erik LU orcid ; Kirkwood, T and Drake, MJ (2006) In Journal of Urology 175(2). p.771-776
Abstract
Purpose: The isolated bladder expresses autonomous activity, which may contribute to the generation of lower urinary tract sensation or pathophysiology. We evaluated how the effect of a cholinergic agonist on autonomous activity alters with increasing volume and in the presence of substances known to modulate functional bladder capacity. Materials and Methods: The bladder of 22 adult female C57 black mice were mounted in whole organ tissue baths. Recordings of intravesical pressure were performed under standardized conditions at different bladder volumes. Results: At low volume the muscarinic agonist arecaidine elicited an initial peak response, which subsided to a sustained steady state pressure. At high volume phasic pressure... (More)
Purpose: The isolated bladder expresses autonomous activity, which may contribute to the generation of lower urinary tract sensation or pathophysiology. We evaluated how the effect of a cholinergic agonist on autonomous activity alters with increasing volume and in the presence of substances known to modulate functional bladder capacity. Materials and Methods: The bladder of 22 adult female C57 black mice were mounted in whole organ tissue baths. Recordings of intravesical pressure were performed under standardized conditions at different bladder volumes. Results: At low volume the muscarinic agonist arecaidine elicited an initial peak response, which subsided to a sustained steady state pressure. At high volume phasic pressure fluctuations were also apparent. An M-2-receptor antagonist caused a significantly greater decrease in peak and steady state responses than in pressure fluctuations. An M-3-receptor antagonist decreased all 3 components. alpha, beta-methylene adenosine triphosphate markedly decreased fluctuations, in contrast to norepinephrine, which eliminated the steady state response while preserving fluctuations. Conclusions: The response to cholinergic stimulation of the isolated bladder has 3 components. The initial tonic peak response increases with bladder distention and it is inhibited by M-2 and M-3 muscarinic receptor antagonists. The tonic steady state response does not vary with bladder volume and it is inhibited by M-2 and M-3-receptor antagonists, and by beta(3)-adrenergic receptor agonists. Phasic fluctuations are minimal at low bladder volume, and with alpha, beta-methylene adenosine triphosphate or an M3-receptor antagonist. Thus, the response to cholinergic stimulation varies with bladder volume. It can be differentially modulated by muscarinic antagonists and also by agents acting through nonmuscarinic receptors. (Less)
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
muscle, smooth, mice, bladder, urinary incontinence
in
Journal of Urology
volume
175
issue
2
pages
771 - 776
publisher
Lippincott Williams & Wilkins
external identifiers
  • pmid:16407048
  • wos:000234576200096
  • scopus:30344472440
ISSN
1527-3792
DOI
10.1016/S0022-5347(05)00140-0
language
English
LU publication?
yes
id
d8ff3e58-6917-44e1-84ec-8c7f1fd7403d (old id 419726)
date added to LUP
2016-04-01 15:49:19
date last changed
2020-11-24 01:56:54
@article{d8ff3e58-6917-44e1-84ec-8c7f1fd7403d,
  abstract     = {Purpose: The isolated bladder expresses autonomous activity, which may contribute to the generation of lower urinary tract sensation or pathophysiology. We evaluated how the effect of a cholinergic agonist on autonomous activity alters with increasing volume and in the presence of substances known to modulate functional bladder capacity. Materials and Methods: The bladder of 22 adult female C57 black mice were mounted in whole organ tissue baths. Recordings of intravesical pressure were performed under standardized conditions at different bladder volumes. Results: At low volume the muscarinic agonist arecaidine elicited an initial peak response, which subsided to a sustained steady state pressure. At high volume phasic pressure fluctuations were also apparent. An M-2-receptor antagonist caused a significantly greater decrease in peak and steady state responses than in pressure fluctuations. An M-3-receptor antagonist decreased all 3 components. alpha, beta-methylene adenosine triphosphate markedly decreased fluctuations, in contrast to norepinephrine, which eliminated the steady state response while preserving fluctuations. Conclusions: The response to cholinergic stimulation of the isolated bladder has 3 components. The initial tonic peak response increases with bladder distention and it is inhibited by M-2 and M-3 muscarinic receptor antagonists. The tonic steady state response does not vary with bladder volume and it is inhibited by M-2 and M-3-receptor antagonists, and by beta(3)-adrenergic receptor agonists. Phasic fluctuations are minimal at low bladder volume, and with alpha, beta-methylene adenosine triphosphate or an M3-receptor antagonist. Thus, the response to cholinergic stimulation varies with bladder volume. It can be differentially modulated by muscarinic antagonists and also by agents acting through nonmuscarinic receptors.},
  author       = {Lagou, M and Gillespie, JI and Andersson, Karl-Erik and Kirkwood, T and Drake, MJ},
  issn         = {1527-3792},
  language     = {eng},
  number       = {2},
  pages        = {771--776},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Journal of Urology},
  title        = {Bladder volume alters cholinergic responses of the isolated whole mouse bladder},
  url          = {http://dx.doi.org/10.1016/S0022-5347(05)00140-0},
  doi          = {10.1016/S0022-5347(05)00140-0},
  volume       = {175},
  year         = {2006},
}