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Prussian blue-glutamate oxidase modified glassy carbon electrode: A sensitive L-glutamate and beta-N-oxalyl-alpha-beta-diaminopropionic acid (beta-ODAP) sensor

Varma, Shailly LU ; Yigzaw, Yirgalem LU and Gorton, Lo LU (2006) In Analytica Chimica Acta 556(2). p.319-325
Abstract
A sensitive biosensor has been developed for the neurotoxin beta-N-oxalyl-alpha,beta-diaminopropionic acid (beta-ODAP) contained in the seeds of grass pea (Lathyrus sativus) and for L-glutamate based on glutamate oxidase (GlOx) and a Prussian blue (PB) modified glassy carbon (GC) electrode. The configuration of the system is so as to detect the hydrogen peroxide released during the enzymatic cycle at a low applied potential, -50 mV versus Ag vertical bar AgCl, in the flow injection mode. For this purpose GlOx was coupled to PB electrodeposited onto a glassy carbon electrode and stabilised by treatment with tetrabutylammonium toluene-4-sulfonate (TTS) during one of the steps in the electrodeposition. GlOx was cross-linked with... (More)
A sensitive biosensor has been developed for the neurotoxin beta-N-oxalyl-alpha,beta-diaminopropionic acid (beta-ODAP) contained in the seeds of grass pea (Lathyrus sativus) and for L-glutamate based on glutamate oxidase (GlOx) and a Prussian blue (PB) modified glassy carbon (GC) electrode. The configuration of the system is so as to detect the hydrogen peroxide released during the enzymatic cycle at a low applied potential, -50 mV versus Ag vertical bar AgCl, in the flow injection mode. For this purpose GlOx was coupled to PB electrodeposited onto a glassy carbon electrode and stabilised by treatment with tetrabutylammonium toluene-4-sulfonate (TTS) during one of the steps in the electrodeposition. GlOx was cross-linked with glutaraldehyde (GA), bovine serum albumin (BSA) and Tween-20 on the surface of the PB modified GC electrodes. Addition of 0.01% and 0.001% polyethyleneimine (PEI) to the immobilisation mixture resulted in an enhancement of the response signal with about 35% and 62% for glutamate and P-ODAP, respectively, when using 0.01% PEI and with 164% and 200% for glutamate and P-ODAP, respectively, when using 0.001% PEI. The linear response range for P-ODAP was extended from 0.05-0.5 mM to 0.01-1 mM, when 0.001% PEI was used. However, a higher concentration of PEI, 0.1%, caused a decrease in the sensitivity of the biosensor. (c) 2005 Elsevier B.V. All rights reserved. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Prussian blue, electrodes, enzyme modified, polyethyleneimine, glutamate oxidase, beta-ODAP
in
Analytica Chimica Acta
volume
556
issue
2
pages
319 - 325
publisher
Elsevier
external identifiers
  • wos:000234729700010
  • scopus:29944441490
ISSN
1873-4324
DOI
10.1016/j.aca.2005.09.047
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Department of Chemistry (011001220)
id
9e83a1e9-23d2-48ca-9316-48fa918d74ae (old id 419999)
date added to LUP
2016-04-01 16:37:04
date last changed
2021-02-17 06:04:46
@article{9e83a1e9-23d2-48ca-9316-48fa918d74ae,
  abstract     = {A sensitive biosensor has been developed for the neurotoxin beta-N-oxalyl-alpha,beta-diaminopropionic acid (beta-ODAP) contained in the seeds of grass pea (Lathyrus sativus) and for L-glutamate based on glutamate oxidase (GlOx) and a Prussian blue (PB) modified glassy carbon (GC) electrode. The configuration of the system is so as to detect the hydrogen peroxide released during the enzymatic cycle at a low applied potential, -50 mV versus Ag vertical bar AgCl, in the flow injection mode. For this purpose GlOx was coupled to PB electrodeposited onto a glassy carbon electrode and stabilised by treatment with tetrabutylammonium toluene-4-sulfonate (TTS) during one of the steps in the electrodeposition. GlOx was cross-linked with glutaraldehyde (GA), bovine serum albumin (BSA) and Tween-20 on the surface of the PB modified GC electrodes. Addition of 0.01% and 0.001% polyethyleneimine (PEI) to the immobilisation mixture resulted in an enhancement of the response signal with about 35% and 62% for glutamate and P-ODAP, respectively, when using 0.01% PEI and with 164% and 200% for glutamate and P-ODAP, respectively, when using 0.001% PEI. The linear response range for P-ODAP was extended from 0.05-0.5 mM to 0.01-1 mM, when 0.001% PEI was used. However, a higher concentration of PEI, 0.1%, caused a decrease in the sensitivity of the biosensor. (c) 2005 Elsevier B.V. All rights reserved.},
  author       = {Varma, Shailly and Yigzaw, Yirgalem and Gorton, Lo},
  issn         = {1873-4324},
  language     = {eng},
  number       = {2},
  pages        = {319--325},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {Prussian blue-glutamate oxidase modified glassy carbon electrode: A sensitive L-glutamate and beta-N-oxalyl-alpha-beta-diaminopropionic acid (beta-ODAP) sensor},
  url          = {http://dx.doi.org/10.1016/j.aca.2005.09.047},
  doi          = {10.1016/j.aca.2005.09.047},
  volume       = {556},
  year         = {2006},
}