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Human class II major histocompatibility antigen β-chains are derived from at least three loci

Böhme, Jan ; Owerbach, David ; Denaro, Maurizio ; Lernmark, Åke LU ; Peterson, Per A. and Rask, Lars (1983) In Nature 301(5895). p.82-84
Abstract

Class II antigens of the major histocompatibility complex (MHC) consist of two glycosylated, membrane-integrated polypeptide chains1. These cell surface-expressed molecules are involved in several immunobiological events involving cell-cell interactions2,3, most of which seem to require that genetically identical class II antigens, or other molecules controlled by the same region of the MHC, are expressed on the interacting cells4. The extensive genetic polymorphism of the class II antigens5 has rendered analyses in the human system of the number of non-allelic species of class II antigens difficult, although several laboratories have reported the existence of at least two types of human class... (More)

Class II antigens of the major histocompatibility complex (MHC) consist of two glycosylated, membrane-integrated polypeptide chains1. These cell surface-expressed molecules are involved in several immunobiological events involving cell-cell interactions2,3, most of which seem to require that genetically identical class II antigens, or other molecules controlled by the same region of the MHC, are expressed on the interacting cells4. The extensive genetic polymorphism of the class II antigens5 has rendered analyses in the human system of the number of non-allelic species of class II antigens difficult, although several laboratories have reported the existence of at least two types of human class II antigens6-9. Here we present the results of experiments using restriction enzyme digestions and separation of DNA from individuals homozygous for the MHC followed by hybridization to human class II antigen α-10,11 and β-12-14 chain cDNA probes. While the α -chain probe gave only a single hybridization band, the various β -chain probes revealed a more complex pattern that is consistent with the existence of at least three separate β -chain genes or pseudogenes in the human MHC.

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published
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in
Nature
volume
301
issue
5895
pages
3 pages
publisher
Nature Publishing Group
external identifiers
  • scopus:0020679853
  • pmid:6296686
ISSN
0028-0836
DOI
10.1038/301082a0
language
English
LU publication?
no
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41be168c-aa71-4f23-9a16-2e2d91799192
date added to LUP
2019-12-12 23:23:10
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2020-01-13 02:36:24
@article{41be168c-aa71-4f23-9a16-2e2d91799192,
  abstract     = {<p>Class II antigens of the major histocompatibility complex (MHC) consist of two glycosylated, membrane-integrated polypeptide chains<sup>1</sup>. These cell surface-expressed molecules are involved in several immunobiological events involving cell-cell interactions<sup>2,3</sup>, most of which seem to require that genetically identical class II antigens, or other molecules controlled by the same region of the MHC, are expressed on the interacting cells<sup>4</sup>. The extensive genetic polymorphism of the class II antigens<sup>5</sup> has rendered analyses in the human system of the number of non-allelic species of class II antigens difficult, although several laboratories have reported the existence of at least two types of human class II antigens<sup>6-9</sup>. Here we present the results of experiments using restriction enzyme digestions and separation of DNA from individuals homozygous for the MHC followed by hybridization to human class II antigen α-<sup>10,11</sup> and β-<sup>12-14</sup> chain cDNA probes. While the α -chain probe gave only a single hybridization band, the various β -chain probes revealed a more complex pattern that is consistent with the existence of at least three separate β -chain genes or pseudogenes in the human MHC.</p>},
  author       = {Böhme, Jan and Owerbach, David and Denaro, Maurizio and Lernmark, Åke and Peterson, Per A. and Rask, Lars},
  issn         = {0028-0836},
  language     = {eng},
  month        = {12},
  number       = {5895},
  pages        = {82--84},
  publisher    = {Nature Publishing Group},
  series       = {Nature},
  title        = {Human class II major histocompatibility antigen β-chains are derived from at least three loci},
  url          = {http://dx.doi.org/10.1038/301082a0},
  doi          = {10.1038/301082a0},
  volume       = {301},
  year         = {1983},
}