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Remoção da cartilagem articular associada ou não a implante homógeno ou enxerto autógeno de osso esponjoso em cães submetidos à artrodese atlantoaxial

Festugatto, Rafael ; Mazzanti, Alexandre ; Raiser, Alceu Gaspar ; da Silva, Fernanda Souza Barbosa ; Treiche, Tiago Luis Eilers ; da Cunha, João Paulo Mori LU ; Muler, Fernanda Cátia ; Vasconcellos, Jaine Soares de Paula ; Veiga, Denise de Castro and Anjos, Bruno Leite Dos (2013) In Ciencia Rural 43(3). p.466-473
Abstract

The aim of this study was to evaluate the degree of joint fusion and bone formation in dogs undergoing atlantoaxial arthrodesis after removal of articular cartilage associated or not to implant homogenous or autogenous cancellous bone. Twelve dogs, weighing between 8 and 12kg were randomly divided into three groups. Group I (GI) performed only the removal of joint cartilage and joint immobilization with acrylic resin and pins. Group II (GII): after removel of joint cartilage and articular immobilization was performed modeling and placement of homogenous cancellous bone at the given location. The volume of homograft placed in the joint was measured using a precision balance and all animals received the same amount. Group III (GIII): this... (More)

The aim of this study was to evaluate the degree of joint fusion and bone formation in dogs undergoing atlantoaxial arthrodesis after removal of articular cartilage associated or not to implant homogenous or autogenous cancellous bone. Twelve dogs, weighing between 8 and 12kg were randomly divided into three groups. Group I (GI) performed only the removal of joint cartilage and joint immobilization with acrylic resin and pins. Group II (GII): after removel of joint cartilage and articular immobilization was performed modeling and placement of homogenous cancellous bone at the given location. The volume of homograft placed in the joint was measured using a precision balance and all animals received the same amount. Group III (GIII): this was the same procedure as GI, however, was used autogenous cancellous bone graft at the given location. Radiographic examinations were performed on all animals at 30, 60 and 90 days postoperatively. At 90 days of PO was carried out euthanasia of animals for testing of manual palpation and CT evaluation and histological. Samples were kept in solution in 10% buffered formaldehyde for fixation for a minimum of 72 hours and after, decalcified in formic acid and sodium citrate for subsequent processing and included in paraffin. Sections were stained with hematoxylin-eosin for light microscopy evaluation. Statistical analysis of the association between the degree of joint fusion assessed by manual palpation, the digitized radiographic images and CT scans and treatment groups, we applied the Chi-square test of independence. The test results were evaluated by the exact significance and considered statistically significant at 5% significance (P<0.05). By manual palpation test and by the CT images can be seen that there was no statistically significant difference between groups at 90 days postoperatively. Radiographic analysis of the atlantoaxial joint showed that the degree of fusion was similar among treatments, no statistical difference at 30, 60 and 90 days postoperatively. Regarding the histopathological study of atlantoaxial joint of dogs after 90 days postoperatively was found that bone formation in group I had 25% of each intensity (absent, mild, moderate, severe) in group II, 75% lighter and 25% sharp and in group III, 25% moderate and 75% severe. It can be concluded that the use of graft homogenous preserved in 98% glycerin in dogs undergoing atlantoaxial fusion method is a viable alternative for treatment of atlantoaxial instability. There is no difference in the degree of joint fusion and bone formation when the technique of atlantoaxial arthrodesis in dogs is associated with autogenous cancellous bone graft or implant homogenous. The removal of articular cartilage alone is an effective and shows similar results to the use of autograft or homogenous implant.

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author
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alternative title
Joint cartilage removal associated or not to homologous implant or autologous cancellous bone graft in dogs submitted to atlantoaxial arthrodesis
publishing date
type
Contribution to journal
publication status
published
keywords
Atlantoaxial instability, Cancellous bone, Dog, Neurology
in
Ciencia Rural
volume
43
issue
3
pages
466 - 473
publisher
Universidade Federal de Santa Maria
external identifiers
  • scopus:84875203335
ISSN
0103-8478
DOI
10.1590/S0103-84782013000300015
language
Portuguese
LU publication?
no
id
41cb8300-286b-409b-a6be-8ccb60d6d4e7
date added to LUP
2019-09-18 13:47:21
date last changed
2020-01-13 02:23:41
@article{41cb8300-286b-409b-a6be-8ccb60d6d4e7,
  abstract     = {<p>The aim of this study was to evaluate the degree of joint fusion and bone formation in dogs undergoing atlantoaxial arthrodesis after removal of articular cartilage associated or not to implant homogenous or autogenous cancellous bone. Twelve dogs, weighing between 8 and 12kg were randomly divided into three groups. Group I (GI) performed only the removal of joint cartilage and joint immobilization with acrylic resin and pins. Group II (GII): after removel of joint cartilage and articular immobilization was performed modeling and placement of homogenous cancellous bone at the given location. The volume of homograft placed in the joint was measured using a precision balance and all animals received the same amount. Group III (GIII): this was the same procedure as GI, however, was used autogenous cancellous bone graft at the given location. Radiographic examinations were performed on all animals at 30, 60 and 90 days postoperatively. At 90 days of PO was carried out euthanasia of animals for testing of manual palpation and CT evaluation and histological. Samples were kept in solution in 10% buffered formaldehyde for fixation for a minimum of 72 hours and after, decalcified in formic acid and sodium citrate for subsequent processing and included in paraffin. Sections were stained with hematoxylin-eosin for light microscopy evaluation. Statistical analysis of the association between the degree of joint fusion assessed by manual palpation, the digitized radiographic images and CT scans and treatment groups, we applied the Chi-square test of independence. The test results were evaluated by the exact significance and considered statistically significant at 5% significance (P&lt;0.05). By manual palpation test and by the CT images can be seen that there was no statistically significant difference between groups at 90 days postoperatively. Radiographic analysis of the atlantoaxial joint showed that the degree of fusion was similar among treatments, no statistical difference at 30, 60 and 90 days postoperatively. Regarding the histopathological study of atlantoaxial joint of dogs after 90 days postoperatively was found that bone formation in group I had 25% of each intensity (absent, mild, moderate, severe) in group II, 75% lighter and 25% sharp and in group III, 25% moderate and 75% severe. It can be concluded that the use of graft homogenous preserved in 98% glycerin in dogs undergoing atlantoaxial fusion method is a viable alternative for treatment of atlantoaxial instability. There is no difference in the degree of joint fusion and bone formation when the technique of atlantoaxial arthrodesis in dogs is associated with autogenous cancellous bone graft or implant homogenous. The removal of articular cartilage alone is an effective and shows similar results to the use of autograft or homogenous implant.</p>},
  author       = {Festugatto, Rafael and Mazzanti, Alexandre and Raiser, Alceu Gaspar and da Silva, Fernanda Souza Barbosa and Treiche, Tiago Luis Eilers and da Cunha, João Paulo Mori and Muler, Fernanda Cátia and Vasconcellos, Jaine Soares de Paula and Veiga, Denise de Castro and Anjos, Bruno Leite Dos},
  issn         = {0103-8478},
  language     = {por},
  number       = {3},
  pages        = {466--473},
  publisher    = {Universidade Federal de Santa Maria},
  series       = {Ciencia Rural},
  title        = {Remoção da cartilagem articular associada ou não a implante homógeno ou enxerto autógeno de osso esponjoso em cães submetidos à artrodese atlantoaxial},
  url          = {http://dx.doi.org/10.1590/S0103-84782013000300015},
  doi          = {10.1590/S0103-84782013000300015},
  volume       = {43},
  year         = {2013},
}