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Progressive improvement in wound healing with increased therapy in haemophilia B mice

Monroe, D. M. ; Hoffman, M. ; Roberts, H. R. and Hedner, Ulla LU (2013) In Haemophilia 19(6). p.926-932
Abstract
Previous work has shown that normalized haemostasis only at the time of an injury is not sufficient to promote optimal wound healing in haemophilia B (HB) mice. However, the duration of treatment required for optimal healing has not been established. The goal of these studies was to determine the effect of different durations of replacement or bypassing therapy [factor IX(FIX) or factor VIIa (FVIIa)] on wound healing parameters in a mouse model of HB. A dermal wound was placed on the back of HB mice. Animals were either untreated or pretreated and then subsequently treated for 3days, 5days, or 7days with FIX or FVIIa. Wound area, time to wound healing, haematoma formation and iron deposition were measured. All treated animals showed... (More)
Previous work has shown that normalized haemostasis only at the time of an injury is not sufficient to promote optimal wound healing in haemophilia B (HB) mice. However, the duration of treatment required for optimal healing has not been established. The goal of these studies was to determine the effect of different durations of replacement or bypassing therapy [factor IX(FIX) or factor VIIa (FVIIa)] on wound healing parameters in a mouse model of HB. A dermal wound was placed on the back of HB mice. Animals were either untreated or pretreated and then subsequently treated for 3days, 5days, or 7days with FIX or FVIIa. Wound area, time to wound healing, haematoma formation and iron deposition were measured. All treated animals showed shortened time to healing relative to untreated animals. Haematoma formation was prevented by treatment and bleeding into the wounds, measured by iron scores, was reduced by treatment. In addition, there was a progressive improvement in healing with 7days of treatment more effective than 5days which was more effective than 3days. Replacement therapy with FIX had slightly shorter healing times than bypassing therapy with FVIIa. HB mice treated with FIX had slightly smaller wound area than untreated animals; by contrast, FVIIa-treated animals had much smaller wound areas that were close to the wound areas seen in wild-type animals. The data suggest that sustained therapy is required for normal wound healing. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bleeding, factor IX, factor VIIa, haemophilia, mouse models, wound, healing
in
Haemophilia
volume
19
issue
6
pages
926 - 932
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • wos:000326105100042
  • scopus:84886641038
  • pmid:23879625
ISSN
1351-8216
DOI
10.1111/hae.12220
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Emergency medicine/Medicine/Surgery (013240200)
id
d104bf08-74af-43a6-9b33-ed6b37588237 (old id 4204511)
date added to LUP
2016-04-01 09:51:33
date last changed
2019-08-07 01:01:42
@article{d104bf08-74af-43a6-9b33-ed6b37588237,
  abstract     = {Previous work has shown that normalized haemostasis only at the time of an injury is not sufficient to promote optimal wound healing in haemophilia B (HB) mice. However, the duration of treatment required for optimal healing has not been established. The goal of these studies was to determine the effect of different durations of replacement or bypassing therapy [factor IX(FIX) or factor VIIa (FVIIa)] on wound healing parameters in a mouse model of HB. A dermal wound was placed on the back of HB mice. Animals were either untreated or pretreated and then subsequently treated for 3days, 5days, or 7days with FIX or FVIIa. Wound area, time to wound healing, haematoma formation and iron deposition were measured. All treated animals showed shortened time to healing relative to untreated animals. Haematoma formation was prevented by treatment and bleeding into the wounds, measured by iron scores, was reduced by treatment. In addition, there was a progressive improvement in healing with 7days of treatment more effective than 5days which was more effective than 3days. Replacement therapy with FIX had slightly shorter healing times than bypassing therapy with FVIIa. HB mice treated with FIX had slightly smaller wound area than untreated animals; by contrast, FVIIa-treated animals had much smaller wound areas that were close to the wound areas seen in wild-type animals. The data suggest that sustained therapy is required for normal wound healing.},
  author       = {Monroe, D. M. and Hoffman, M. and Roberts, H. R. and Hedner, Ulla},
  issn         = {1351-8216},
  language     = {eng},
  number       = {6},
  pages        = {926--932},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {Haemophilia},
  title        = {Progressive improvement in wound healing with increased therapy in haemophilia B mice},
  url          = {http://dx.doi.org/10.1111/hae.12220},
  doi          = {10.1111/hae.12220},
  volume       = {19},
  year         = {2013},
}