Immobilised peptide displaying phages as affinity ligands purification of lactoferrin from defatted milk
(2006) In Journal of Chromatography A 1101(1-2). p.79-85- Abstract
- An affinity purification procedure for the direct purification of lactoferrin from defatted (skimmed) milk has been developed. The procedure is based on using selected phage clones expressing a peptide with high binding affinity for lactoferrin which were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10-100 mu m) of macroporous poly(dimethylacrylamide) makes it possible to couple long (1 mu m) phage particles as ligands without any risk of blocking the monolithic column. Bound lactoferrin was eluted using 1 M NaC1 with a purity of > 95%. The technique presents a good alternative to conventional immunoaffinity chromatography for purification of a protein of interest from complex samples... (More)
- An affinity purification procedure for the direct purification of lactoferrin from defatted (skimmed) milk has been developed. The procedure is based on using selected phage clones expressing a peptide with high binding affinity for lactoferrin which were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10-100 mu m) of macroporous poly(dimethylacrylamide) makes it possible to couple long (1 mu m) phage particles as ligands without any risk of blocking the monolithic column. Bound lactoferrin was eluted using 1 M NaC1 with a purity of > 95%. The technique presents a good alternative to conventional immunoaffinity chromatography for purification of a protein of interest from complex samples due to (i) the robustness of the system in terms of recovery and ligand leakage and (ii) economical aspect in terms of low ligand cost. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/421315
- author
- Noppe, W ; Plieva, Fatima LU ; Galaev, Igor LU ; Vanhoorelbeke, K ; Mattiasson, Bo LU and Deckmyn, H
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- purification, lactoferrin, macroporous monolithic gels, phage clones
- in
- Journal of Chromatography A
- volume
- 1101
- issue
- 1-2
- pages
- 79 - 85
- publisher
- Elsevier
- external identifiers
-
- pmid:16216254
- wos:000234639600009
- scopus:28944452854
- ISSN
- 0021-9673
- DOI
- 10.1016/j.chroma.2005.09.064
- language
- English
- LU publication?
- yes
- id
- 17c4548e-0aa9-4ddc-b314-77662a3d6303 (old id 421315)
- date added to LUP
- 2016-04-01 17:11:52
- date last changed
- 2022-03-15 05:37:37
@article{17c4548e-0aa9-4ddc-b314-77662a3d6303,
abstract = {{An affinity purification procedure for the direct purification of lactoferrin from defatted (skimmed) milk has been developed. The procedure is based on using selected phage clones expressing a peptide with high binding affinity for lactoferrin which were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10-100 mu m) of macroporous poly(dimethylacrylamide) makes it possible to couple long (1 mu m) phage particles as ligands without any risk of blocking the monolithic column. Bound lactoferrin was eluted using 1 M NaC1 with a purity of > 95%. The technique presents a good alternative to conventional immunoaffinity chromatography for purification of a protein of interest from complex samples due to (i) the robustness of the system in terms of recovery and ligand leakage and (ii) economical aspect in terms of low ligand cost.}},
author = {{Noppe, W and Plieva, Fatima and Galaev, Igor and Vanhoorelbeke, K and Mattiasson, Bo and Deckmyn, H}},
issn = {{0021-9673}},
keywords = {{purification; lactoferrin; macroporous monolithic gels; phage clones}},
language = {{eng}},
number = {{1-2}},
pages = {{79--85}},
publisher = {{Elsevier}},
series = {{Journal of Chromatography A}},
title = {{Immobilised peptide displaying phages as affinity ligands purification of lactoferrin from defatted milk}},
url = {{http://dx.doi.org/10.1016/j.chroma.2005.09.064}},
doi = {{10.1016/j.chroma.2005.09.064}},
volume = {{1101}},
year = {{2006}},
}