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Decreased expression of mannose-specific adhesins by Escherichia coli in the colonic microflora of immunoglobulin A-deficient individuals

Friman, Vanda ; Adlerberth, Ingegerd ; Connell, Hugh LU ; Svanborg, Catharina LU ; Hanson, Lars Å and Wold, Agnes E. (1996) In Infection and Immunity 64(7). p.2794-2798
Abstract

Most Escherichia coli isolates can express type 1 fimbriae with mannose- specific adhesins. These adhesins bind to the oligosaccharide chains of secretory immunoglobulin A (IgA). Thus, in addition to specific antibody activity, secretory IgA possesses a broad reactivity with bacteria expressing type I fimbriae. The absence of secretory IgA in colonic secretions, as seen in IgA deficiency, might therefore alter the ability of type 1-fimbriated E. coli to colonize the large intestines of these individuals. In the present study, 10 E. coli isolates from each of 17 IgA-deficient and 17 age-matched control individuals were assessed for the carriage of the fim gene cluster by DNA-DNA hybridization and for the expression of type 1 fimbriae by... (More)

Most Escherichia coli isolates can express type 1 fimbriae with mannose- specific adhesins. These adhesins bind to the oligosaccharide chains of secretory immunoglobulin A (IgA). Thus, in addition to specific antibody activity, secretory IgA possesses a broad reactivity with bacteria expressing type I fimbriae. The absence of secretory IgA in colonic secretions, as seen in IgA deficiency, might therefore alter the ability of type 1-fimbriated E. coli to colonize the large intestines of these individuals. In the present study, 10 E. coli isolates from each of 17 IgA-deficient and 17 age-matched control individuals were assessed for the carriage of the fim gene cluster by DNA-DNA hybridization and for the expression of type 1 fimbriae by hemagglutination of guinea pig erythrocytes. The contribution of type 1- fimbria-mediated adherence to HT-29 colonic cells was also analyzed. The proportion of fim+ E. coli isolates was lower in IgA-deficient than in control individuals (74 versus 94%, P < 0.05), as was the proportion of isolates expressing type 1 fimbriae in vitro (69% versus 85%, P < 0.05). The median mannose-sensitive adherence to HT-29 cells was lower for isolates from IgA-deficient individuals than from the controls (9 versus 26 bacteria per cell, P < 0.05). Isolates expressing type 1 fimbriae showed lower adherence to HT-29 cells when they were derived from IgA-deficient individuals than when they were derived from control individuals (115 versus 27 bacteria per cell, P < 0.05). The results suggest that the interaction of type 1 fimbriae with secretory IgA contributes to the large intestinal colonization by these bacteria.

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Contribution to journal
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published
in
Infection and Immunity
volume
64
issue
7
pages
5 pages
publisher
American Society for Microbiology
external identifiers
  • pmid:8698510
  • scopus:0029976479
ISSN
0019-9567
language
English
LU publication?
yes
id
4222eab2-2ccb-451a-8b44-2a37352378b4
date added to LUP
2019-06-19 14:17:20
date last changed
2024-04-02 08:50:05
@article{4222eab2-2ccb-451a-8b44-2a37352378b4,
  abstract     = {{<p>Most Escherichia coli isolates can express type 1 fimbriae with mannose- specific adhesins. These adhesins bind to the oligosaccharide chains of secretory immunoglobulin A (IgA). Thus, in addition to specific antibody activity, secretory IgA possesses a broad reactivity with bacteria expressing type I fimbriae. The absence of secretory IgA in colonic secretions, as seen in IgA deficiency, might therefore alter the ability of type 1-fimbriated E. coli to colonize the large intestines of these individuals. In the present study, 10 E. coli isolates from each of 17 IgA-deficient and 17 age-matched control individuals were assessed for the carriage of the fim gene cluster by DNA-DNA hybridization and for the expression of type 1 fimbriae by hemagglutination of guinea pig erythrocytes. The contribution of type 1- fimbria-mediated adherence to HT-29 colonic cells was also analyzed. The proportion of fim<sup>+</sup> E. coli isolates was lower in IgA-deficient than in control individuals (74 versus 94%, P &lt; 0.05), as was the proportion of isolates expressing type 1 fimbriae in vitro (69% versus 85%, P &lt; 0.05). The median mannose-sensitive adherence to HT-29 cells was lower for isolates from IgA-deficient individuals than from the controls (9 versus 26 bacteria per cell, P &lt; 0.05). Isolates expressing type 1 fimbriae showed lower adherence to HT-29 cells when they were derived from IgA-deficient individuals than when they were derived from control individuals (115 versus 27 bacteria per cell, P &lt; 0.05). The results suggest that the interaction of type 1 fimbriae with secretory IgA contributes to the large intestinal colonization by these bacteria.</p>}},
  author       = {{Friman, Vanda and Adlerberth, Ingegerd and Connell, Hugh and Svanborg, Catharina and Hanson, Lars Å and Wold, Agnes E.}},
  issn         = {{0019-9567}},
  language     = {{eng}},
  month        = {{07}},
  number       = {{7}},
  pages        = {{2794--2798}},
  publisher    = {{American Society for Microbiology}},
  series       = {{Infection and Immunity}},
  title        = {{Decreased expression of mannose-specific adhesins by Escherichia coli in the colonic microflora of immunoglobulin A-deficient individuals}},
  volume       = {{64}},
  year         = {{1996}},
}