Decreased expression of mannose-specific adhesins by Escherichia coli in the colonic microflora of immunoglobulin A-deficient individuals
(1996) In Infection and Immunity 64(7). p.2794-2798- Abstract
Most Escherichia coli isolates can express type 1 fimbriae with mannose- specific adhesins. These adhesins bind to the oligosaccharide chains of secretory immunoglobulin A (IgA). Thus, in addition to specific antibody activity, secretory IgA possesses a broad reactivity with bacteria expressing type I fimbriae. The absence of secretory IgA in colonic secretions, as seen in IgA deficiency, might therefore alter the ability of type 1-fimbriated E. coli to colonize the large intestines of these individuals. In the present study, 10 E. coli isolates from each of 17 IgA-deficient and 17 age-matched control individuals were assessed for the carriage of the fim gene cluster by DNA-DNA hybridization and for the expression of type 1 fimbriae by... (More)
Most Escherichia coli isolates can express type 1 fimbriae with mannose- specific adhesins. These adhesins bind to the oligosaccharide chains of secretory immunoglobulin A (IgA). Thus, in addition to specific antibody activity, secretory IgA possesses a broad reactivity with bacteria expressing type I fimbriae. The absence of secretory IgA in colonic secretions, as seen in IgA deficiency, might therefore alter the ability of type 1-fimbriated E. coli to colonize the large intestines of these individuals. In the present study, 10 E. coli isolates from each of 17 IgA-deficient and 17 age-matched control individuals were assessed for the carriage of the fim gene cluster by DNA-DNA hybridization and for the expression of type 1 fimbriae by hemagglutination of guinea pig erythrocytes. The contribution of type 1- fimbria-mediated adherence to HT-29 colonic cells was also analyzed. The proportion of fim+ E. coli isolates was lower in IgA-deficient than in control individuals (74 versus 94%, P < 0.05), as was the proportion of isolates expressing type 1 fimbriae in vitro (69% versus 85%, P < 0.05). The median mannose-sensitive adherence to HT-29 cells was lower for isolates from IgA-deficient individuals than from the controls (9 versus 26 bacteria per cell, P < 0.05). Isolates expressing type 1 fimbriae showed lower adherence to HT-29 cells when they were derived from IgA-deficient individuals than when they were derived from control individuals (115 versus 27 bacteria per cell, P < 0.05). The results suggest that the interaction of type 1 fimbriae with secretory IgA contributes to the large intestinal colonization by these bacteria.
(Less)
- author
- Friman, Vanda ; Adlerberth, Ingegerd ; Connell, Hugh LU ; Svanborg, Catharina LU ; Hanson, Lars Å and Wold, Agnes E.
- organization
- publishing date
- 1996-07-19
- type
- Contribution to journal
- publication status
- published
- in
- Infection and Immunity
- volume
- 64
- issue
- 7
- pages
- 5 pages
- publisher
- American Society for Microbiology
- external identifiers
-
- scopus:0029976479
- pmid:8698510
- ISSN
- 0019-9567
- language
- English
- LU publication?
- yes
- id
- 4222eab2-2ccb-451a-8b44-2a37352378b4
- date added to LUP
- 2019-06-19 14:17:20
- date last changed
- 2025-01-09 15:19:35
@article{4222eab2-2ccb-451a-8b44-2a37352378b4,
abstract = {{<p>Most Escherichia coli isolates can express type 1 fimbriae with mannose- specific adhesins. These adhesins bind to the oligosaccharide chains of secretory immunoglobulin A (IgA). Thus, in addition to specific antibody activity, secretory IgA possesses a broad reactivity with bacteria expressing type I fimbriae. The absence of secretory IgA in colonic secretions, as seen in IgA deficiency, might therefore alter the ability of type 1-fimbriated E. coli to colonize the large intestines of these individuals. In the present study, 10 E. coli isolates from each of 17 IgA-deficient and 17 age-matched control individuals were assessed for the carriage of the fim gene cluster by DNA-DNA hybridization and for the expression of type 1 fimbriae by hemagglutination of guinea pig erythrocytes. The contribution of type 1- fimbria-mediated adherence to HT-29 colonic cells was also analyzed. The proportion of fim<sup>+</sup> E. coli isolates was lower in IgA-deficient than in control individuals (74 versus 94%, P < 0.05), as was the proportion of isolates expressing type 1 fimbriae in vitro (69% versus 85%, P < 0.05). The median mannose-sensitive adherence to HT-29 cells was lower for isolates from IgA-deficient individuals than from the controls (9 versus 26 bacteria per cell, P < 0.05). Isolates expressing type 1 fimbriae showed lower adherence to HT-29 cells when they were derived from IgA-deficient individuals than when they were derived from control individuals (115 versus 27 bacteria per cell, P < 0.05). The results suggest that the interaction of type 1 fimbriae with secretory IgA contributes to the large intestinal colonization by these bacteria.</p>}},
author = {{Friman, Vanda and Adlerberth, Ingegerd and Connell, Hugh and Svanborg, Catharina and Hanson, Lars Å and Wold, Agnes E.}},
issn = {{0019-9567}},
language = {{eng}},
month = {{07}},
number = {{7}},
pages = {{2794--2798}},
publisher = {{American Society for Microbiology}},
series = {{Infection and Immunity}},
title = {{Decreased expression of mannose-specific adhesins by Escherichia coli in the colonic microflora of immunoglobulin A-deficient individuals}},
volume = {{64}},
year = {{1996}},
}