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MALDI-target integrated platform for affinity-captured protein digestion.

Ahmad Tajudin, Asilah LU ; Adler, Belinda LU ; Ekström, Simon LU ; Marko-Varga, György LU ; Malm, Johan LU ; Lilja, Hans LU and Laurell, Thomas (2014) In Analytica Chimica Acta 807(Jan 7). p.1-8
Abstract
To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, digestion and sample cleanup with a direct interface to mass spectrometry is presented. The robustness of the on-ISET protein digestion protocol was validated by MALDI MS analysis of model proteins, ranging from 40fmol to 1pmol per nanovial. On-ISET digestion and MALDI MS/MS analysis of immunoaffinity captured disease-associated biomarker PSA (prostate specific antigen) from human seminal plasma are presented.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytica Chimica Acta
volume
807
issue
Jan 7
pages
1 - 8
publisher
Elsevier
external identifiers
  • wos:000328569500001
  • pmid:24356215
  • scopus:84890437114
ISSN
1873-4324
DOI
10.1016/j.aca.2013.08.051
language
English
LU publication?
yes
id
872d2fec-62a2-474c-9253-60c9aa60cd39 (old id 4223477)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24356215?dopt=Abstract
date added to LUP
2014-01-05 19:35:59
date last changed
2017-07-30 03:05:29
@article{872d2fec-62a2-474c-9253-60c9aa60cd39,
  abstract     = {To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, digestion and sample cleanup with a direct interface to mass spectrometry is presented. The robustness of the on-ISET protein digestion protocol was validated by MALDI MS analysis of model proteins, ranging from 40fmol to 1pmol per nanovial. On-ISET digestion and MALDI MS/MS analysis of immunoaffinity captured disease-associated biomarker PSA (prostate specific antigen) from human seminal plasma are presented.},
  author       = {Ahmad Tajudin, Asilah and Adler, Belinda and Ekström, Simon and Marko-Varga, György and Malm, Johan and Lilja, Hans and Laurell, Thomas},
  issn         = {1873-4324},
  language     = {eng},
  number       = {Jan 7},
  pages        = {1--8},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {MALDI-target integrated platform for affinity-captured protein digestion.},
  url          = {http://dx.doi.org/10.1016/j.aca.2013.08.051},
  volume       = {807},
  year         = {2014},
}