MALDI-target integrated platform for affinity-captured protein digestion.
(2014) In Analytica Chimica Acta 807(Jan 7). p.1-8- Abstract
- To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, digestion and sample cleanup with a direct interface to mass spectrometry is presented. The robustness of the on-ISET protein digestion protocol was validated by MALDI MS analysis of model proteins, ranging from 40fmol to 1pmol per nanovial. On-ISET digestion and MALDI MS/MS analysis of immunoaffinity captured disease-associated biomarker PSA (prostate specific antigen) from human seminal plasma are presented.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4223477
- author
- Ahmad Tajudin, Asilah LU ; Adler, Belinda LU ; Ekström, Simon LU ; Marko-Varga, György LU ; Malm, Johan LU ; Lilja, Hans LU and Laurell, Thomas
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytica Chimica Acta
- volume
- 807
- issue
- Jan 7
- pages
- 1 - 8
- publisher
- Elsevier
- external identifiers
-
- wos:000328569500001
- pmid:24356215
- scopus:84890437114
- pmid:24356215
- ISSN
- 1873-4324
- DOI
- 10.1016/j.aca.2013.08.051
- language
- English
- LU publication?
- yes
- id
- 872d2fec-62a2-474c-9253-60c9aa60cd39 (old id 4223477)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24356215?dopt=Abstract
- date added to LUP
- 2016-04-01 10:02:47
- date last changed
- 2022-02-09 22:11:59
@article{872d2fec-62a2-474c-9253-60c9aa60cd39, abstract = {{To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, digestion and sample cleanup with a direct interface to mass spectrometry is presented. The robustness of the on-ISET protein digestion protocol was validated by MALDI MS analysis of model proteins, ranging from 40fmol to 1pmol per nanovial. On-ISET digestion and MALDI MS/MS analysis of immunoaffinity captured disease-associated biomarker PSA (prostate specific antigen) from human seminal plasma are presented.}}, author = {{Ahmad Tajudin, Asilah and Adler, Belinda and Ekström, Simon and Marko-Varga, György and Malm, Johan and Lilja, Hans and Laurell, Thomas}}, issn = {{1873-4324}}, language = {{eng}}, number = {{Jan 7}}, pages = {{1--8}}, publisher = {{Elsevier}}, series = {{Analytica Chimica Acta}}, title = {{MALDI-target integrated platform for affinity-captured protein digestion.}}, url = {{http://dx.doi.org/10.1016/j.aca.2013.08.051}}, doi = {{10.1016/j.aca.2013.08.051}}, volume = {{807}}, year = {{2014}}, }