Bile Enhances Cell Surface Hydrophobicity and Biofilm Formation of Bifidobacteria.
(2014) In Applied Biochemistry and Biotechnology 172(4). p.1970-1981- Abstract
- Twenty-four human bifidobacterial strains were analysed for cell surface hydrophobicity (CSH) using a salt aggregation test (SAT) and a Congo red binding (CRB) assay. Three strains were selected for a systematic study on the CSH and biofilm formation: Bifidobacterium breve 46, Bifidobacterium animalis ssp. lactis 8:8 and a reference strain B. animalis ssp. lactis JCM 10602. CRB of the B. breve 46 and B. animalis ssp. lactis JCM 10602 was significantly enhanced (P < 0.05) when grown in deMan-Rogosa-Sharpe cysteine (MRSC) broth supplemented with taurocholic acid (TA) or native porcine bile (PB). An enhanced CSH of the strains grown with PB and gastric mucin correlated with an increased mucin binding and an enhanced biofilm formation in... (More)
- Twenty-four human bifidobacterial strains were analysed for cell surface hydrophobicity (CSH) using a salt aggregation test (SAT) and a Congo red binding (CRB) assay. Three strains were selected for a systematic study on the CSH and biofilm formation: Bifidobacterium breve 46, Bifidobacterium animalis ssp. lactis 8:8 and a reference strain B. animalis ssp. lactis JCM 10602. CRB of the B. breve 46 and B. animalis ssp. lactis JCM 10602 was significantly enhanced (P < 0.05) when grown in deMan-Rogosa-Sharpe cysteine (MRSC) broth supplemented with taurocholic acid (TA) or native porcine bile (PB). An enhanced CSH of the strains grown with PB and gastric mucin correlated with an increased mucin binding and an enhanced biofilm formation in prebiotic oligosaccharide-supplemented cultures. The three strains showed late bile-induced biofilm (72 h) under an anaerobic growth condition, and both B. animalis ssp. lactis strains showed a late bile-induced biofilm formation under aerobic conditions shown by crystal violet staining. These two strains were thus considered to be oxygen tolerant and more robust. Furthermore, enhanced biofilm formation of these robust bifidobacterial strains in the presence of prebiotics may allow for strong colonisation in the gastrointestinal tract when administered to in vivo models as a "synbiotic supplement". (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4225014
- author
- Ambalam, Padma ; Kondepudi, Kanthi Kiran ; Nilsson, Ingrid LU ; Wadström, Torkel LU and Ljungh, Åsa LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied Biochemistry and Biotechnology
- volume
- 172
- issue
- 4
- pages
- 1970 - 1981
- publisher
- Humana Press
- external identifiers
-
- pmid:24318587
- wos:000333248000020
- scopus:84896316080
- pmid:24318587
- ISSN
- 1559-0291
- DOI
- 10.1007/s12010-013-0596-1
- language
- English
- LU publication?
- yes
- id
- 81407bc9-8d1d-470e-afd2-a6e95e91e9a2 (old id 4225014)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24318587?dopt=Abstract
- date added to LUP
- 2016-04-01 10:01:44
- date last changed
- 2022-04-19 21:54:39
@article{81407bc9-8d1d-470e-afd2-a6e95e91e9a2, abstract = {{Twenty-four human bifidobacterial strains were analysed for cell surface hydrophobicity (CSH) using a salt aggregation test (SAT) and a Congo red binding (CRB) assay. Three strains were selected for a systematic study on the CSH and biofilm formation: Bifidobacterium breve 46, Bifidobacterium animalis ssp. lactis 8:8 and a reference strain B. animalis ssp. lactis JCM 10602. CRB of the B. breve 46 and B. animalis ssp. lactis JCM 10602 was significantly enhanced (P < 0.05) when grown in deMan-Rogosa-Sharpe cysteine (MRSC) broth supplemented with taurocholic acid (TA) or native porcine bile (PB). An enhanced CSH of the strains grown with PB and gastric mucin correlated with an increased mucin binding and an enhanced biofilm formation in prebiotic oligosaccharide-supplemented cultures. The three strains showed late bile-induced biofilm (72 h) under an anaerobic growth condition, and both B. animalis ssp. lactis strains showed a late bile-induced biofilm formation under aerobic conditions shown by crystal violet staining. These two strains were thus considered to be oxygen tolerant and more robust. Furthermore, enhanced biofilm formation of these robust bifidobacterial strains in the presence of prebiotics may allow for strong colonisation in the gastrointestinal tract when administered to in vivo models as a "synbiotic supplement".}}, author = {{Ambalam, Padma and Kondepudi, Kanthi Kiran and Nilsson, Ingrid and Wadström, Torkel and Ljungh, Åsa}}, issn = {{1559-0291}}, language = {{eng}}, number = {{4}}, pages = {{1970--1981}}, publisher = {{Humana Press}}, series = {{Applied Biochemistry and Biotechnology}}, title = {{Bile Enhances Cell Surface Hydrophobicity and Biofilm Formation of Bifidobacteria.}}, url = {{http://dx.doi.org/10.1007/s12010-013-0596-1}}, doi = {{10.1007/s12010-013-0596-1}}, volume = {{172}}, year = {{2014}}, }