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Bile Enhances Cell Surface Hydrophobicity and Biofilm Formation of Bifidobacteria.

Ambalam, Padma; Kondepudi, Kanthi Kiran; Nilsson, Ingrid LU ; Wadström, Torkel LU and Ljungh, Åsa LU (2014) In Applied Biochemistry and Biotechnology 172(4). p.1970-1981
Abstract
Twenty-four human bifidobacterial strains were analysed for cell surface hydrophobicity (CSH) using a salt aggregation test (SAT) and a Congo red binding (CRB) assay. Three strains were selected for a systematic study on the CSH and biofilm formation: Bifidobacterium breve 46, Bifidobacterium animalis ssp. lactis 8:8 and a reference strain B. animalis ssp. lactis JCM 10602. CRB of the B. breve 46 and B. animalis ssp. lactis JCM 10602 was significantly enhanced (P < 0.05) when grown in deMan-Rogosa-Sharpe cysteine (MRSC) broth supplemented with taurocholic acid (TA) or native porcine bile (PB). An enhanced CSH of the strains grown with PB and gastric mucin correlated with an increased mucin binding and an enhanced biofilm formation in... (More)
Twenty-four human bifidobacterial strains were analysed for cell surface hydrophobicity (CSH) using a salt aggregation test (SAT) and a Congo red binding (CRB) assay. Three strains were selected for a systematic study on the CSH and biofilm formation: Bifidobacterium breve 46, Bifidobacterium animalis ssp. lactis 8:8 and a reference strain B. animalis ssp. lactis JCM 10602. CRB of the B. breve 46 and B. animalis ssp. lactis JCM 10602 was significantly enhanced (P < 0.05) when grown in deMan-Rogosa-Sharpe cysteine (MRSC) broth supplemented with taurocholic acid (TA) or native porcine bile (PB). An enhanced CSH of the strains grown with PB and gastric mucin correlated with an increased mucin binding and an enhanced biofilm formation in prebiotic oligosaccharide-supplemented cultures. The three strains showed late bile-induced biofilm (72 h) under an anaerobic growth condition, and both B. animalis ssp. lactis strains showed a late bile-induced biofilm formation under aerobic conditions shown by crystal violet staining. These two strains were thus considered to be oxygen tolerant and more robust. Furthermore, enhanced biofilm formation of these robust bifidobacterial strains in the presence of prebiotics may allow for strong colonisation in the gastrointestinal tract when administered to in vivo models as a "synbiotic supplement". (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Biochemistry and Biotechnology
volume
172
issue
4
pages
1970 - 1981
publisher
Humana Press
external identifiers
  • pmid:24318587
  • wos:000333248000020
  • scopus:84896316080
ISSN
1559-0291
DOI
10.1007/s12010-013-0596-1
language
English
LU publication?
yes
id
81407bc9-8d1d-470e-afd2-a6e95e91e9a2 (old id 4225014)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24318587?dopt=Abstract
date added to LUP
2014-01-03 14:56:53
date last changed
2017-10-29 03:04:01
@article{81407bc9-8d1d-470e-afd2-a6e95e91e9a2,
  abstract     = {Twenty-four human bifidobacterial strains were analysed for cell surface hydrophobicity (CSH) using a salt aggregation test (SAT) and a Congo red binding (CRB) assay. Three strains were selected for a systematic study on the CSH and biofilm formation: Bifidobacterium breve 46, Bifidobacterium animalis ssp. lactis 8:8 and a reference strain B. animalis ssp. lactis JCM 10602. CRB of the B. breve 46 and B. animalis ssp. lactis JCM 10602 was significantly enhanced (P &lt; 0.05) when grown in deMan-Rogosa-Sharpe cysteine (MRSC) broth supplemented with taurocholic acid (TA) or native porcine bile (PB). An enhanced CSH of the strains grown with PB and gastric mucin correlated with an increased mucin binding and an enhanced biofilm formation in prebiotic oligosaccharide-supplemented cultures. The three strains showed late bile-induced biofilm (72 h) under an anaerobic growth condition, and both B. animalis ssp. lactis strains showed a late bile-induced biofilm formation under aerobic conditions shown by crystal violet staining. These two strains were thus considered to be oxygen tolerant and more robust. Furthermore, enhanced biofilm formation of these robust bifidobacterial strains in the presence of prebiotics may allow for strong colonisation in the gastrointestinal tract when administered to in vivo models as a "synbiotic supplement".},
  author       = {Ambalam, Padma and Kondepudi, Kanthi Kiran and Nilsson, Ingrid and Wadström, Torkel and Ljungh, Åsa},
  issn         = {1559-0291},
  language     = {eng},
  number       = {4},
  pages        = {1970--1981},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Bile Enhances Cell Surface Hydrophobicity and Biofilm Formation of Bifidobacteria.},
  url          = {http://dx.doi.org/10.1007/s12010-013-0596-1},
  volume       = {172},
  year         = {2014},
}