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Plasmid DNA purification using a multimodal Chromatography resin

Matos, Tiago LU ; Queiroz, João A. and Bülow, Leif LU (2014) In Journal of Molecular Recognition 27(4). p.184-189
Abstract (Swedish)
Abstract in Undetermined

Multimodal chromatography is widely used for isolation of proteins because it often results in improved selectivity compared to conventional separation resins. The binding potential and chromatographic behavior of plasmid DNA have here been examined on a Capto Adhere resin. Capto Adhere is a recent multimodal chromatography material allowing molecular recognition between the ligand and target molecule, which is based on combined ionic and aromatic interactions. Capto Adhere proved to offer a very strong binding of nucleic acids. This property could be used to isolate plasmid DNA from a crude Escherichia coli extract. Using a stepwise NaCl gradient, pure plasmid DNA could be obtained without protein... (More)
Abstract in Undetermined

Multimodal chromatography is widely used for isolation of proteins because it often results in improved selectivity compared to conventional separation resins. The binding potential and chromatographic behavior of plasmid DNA have here been examined on a Capto Adhere resin. Capto Adhere is a recent multimodal chromatography material allowing molecular recognition between the ligand and target molecule, which is based on combined ionic and aromatic interactions. Capto Adhere proved to offer a very strong binding of nucleic acids. This property could be used to isolate plasmid DNA from a crude Escherichia coli extract. Using a stepwise NaCl gradient, pure plasmid DNA could be obtained without protein and endotoxin contaminations. The RNA fraction bound most strongly to the resin and could be eluted only at very high salt concentrations (2.0 M NaCl). The chromatographic separation behavior was very robust between pH values 6 and 9, and the dynamic binding capacity was estimated to 60 µg/ml resin. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Molecular Recognition
volume
27
issue
4
pages
184 - 189
publisher
John Wiley & Sons
external identifiers
  • wos:000333021800002
  • pmid:24591175
  • scopus:84897594310
ISSN
1099-1352
DOI
10.1002/jmr.2349
language
English
LU publication?
yes
id
855f4368-72ec-48d3-8580-d33801092481 (old id 4228186)
date added to LUP
2014-01-30 12:12:25
date last changed
2017-09-10 03:25:01
@article{855f4368-72ec-48d3-8580-d33801092481,
  abstract     = {<b>Abstract in Undetermined</b><br/><br>
Multimodal chromatography is widely used for isolation of proteins because it often results in improved selectivity compared to conventional separation resins. The binding potential and chromatographic behavior of plasmid DNA have here been examined on a Capto Adhere resin. Capto Adhere is a recent multimodal chromatography material allowing molecular recognition between the ligand and target molecule, which is based on combined ionic and aromatic interactions. Capto Adhere proved to offer a very strong binding of nucleic acids. This property could be used to isolate plasmid DNA from a crude Escherichia coli extract. Using a stepwise NaCl gradient, pure plasmid DNA could be obtained without protein and endotoxin contaminations. The RNA fraction bound most strongly to the resin and could be eluted only at very high salt concentrations (2.0 M NaCl). The chromatographic separation behavior was very robust between pH values 6 and 9, and the dynamic binding capacity was estimated to 60 µg/ml resin.},
  author       = {Matos, Tiago and Queiroz, João A. and Bülow, Leif},
  issn         = {1099-1352},
  language     = {eng},
  number       = {4},
  pages        = {184--189},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Molecular Recognition},
  title        = {Plasmid DNA purification using a multimodal Chromatography resin},
  url          = {http://dx.doi.org/10.1002/jmr.2349},
  volume       = {27},
  year         = {2014},
}