Plasmid DNA purification using a multimodal Chromatography resin
(2014) In Journal of Molecular Recognition 27(4). p.184-189- Abstract
- Abstract in Undetermined
Multimodal chromatography is widely used for isolation of proteins because it often results in improved selectivity compared to conventional separation resins. The binding potential and chromatographic behavior of plasmid DNA have here been examined on a Capto Adhere resin. Capto Adhere is a recent multimodal chromatography material allowing molecular recognition between the ligand and target molecule, which is based on combined ionic and aromatic interactions. Capto Adhere proved to offer a very strong binding of nucleic acids. This property could be used to isolate plasmid DNA from a crude Escherichia coli extract. Using a stepwise NaCl gradient, pure plasmid DNA could be obtained without protein and... (More) - Abstract in Undetermined
Multimodal chromatography is widely used for isolation of proteins because it often results in improved selectivity compared to conventional separation resins. The binding potential and chromatographic behavior of plasmid DNA have here been examined on a Capto Adhere resin. Capto Adhere is a recent multimodal chromatography material allowing molecular recognition between the ligand and target molecule, which is based on combined ionic and aromatic interactions. Capto Adhere proved to offer a very strong binding of nucleic acids. This property could be used to isolate plasmid DNA from a crude Escherichia coli extract. Using a stepwise NaCl gradient, pure plasmid DNA could be obtained without protein and endotoxin contaminations. The RNA fraction bound most strongly to the resin and could be eluted only at very high salt concentrations (2.0 M NaCl). The chromatographic separation behavior was very robust between pH values 6 and 9, and the dynamic binding capacity was estimated to 60 µg/ml resin. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4228186
- author
- Matos, Tiago LU ; Queiroz, João A. and Bülow, Leif LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Molecular Recognition
- volume
- 27
- issue
- 4
- pages
- 184 - 189
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000333021800002
- pmid:24591175
- scopus:84897594310
- pmid:24591175
- ISSN
- 1099-1352
- DOI
- 10.1002/jmr.2349
- language
- English
- LU publication?
- yes
- id
- 855f4368-72ec-48d3-8580-d33801092481 (old id 4228186)
- date added to LUP
- 2016-04-01 10:59:50
- date last changed
- 2025-01-14 04:05:13
@article{855f4368-72ec-48d3-8580-d33801092481, abstract = {{Abstract in Undetermined<br/>Multimodal chromatography is widely used for isolation of proteins because it often results in improved selectivity compared to conventional separation resins. The binding potential and chromatographic behavior of plasmid DNA have here been examined on a Capto Adhere resin. Capto Adhere is a recent multimodal chromatography material allowing molecular recognition between the ligand and target molecule, which is based on combined ionic and aromatic interactions. Capto Adhere proved to offer a very strong binding of nucleic acids. This property could be used to isolate plasmid DNA from a crude Escherichia coli extract. Using a stepwise NaCl gradient, pure plasmid DNA could be obtained without protein and endotoxin contaminations. The RNA fraction bound most strongly to the resin and could be eluted only at very high salt concentrations (2.0 M NaCl). The chromatographic separation behavior was very robust between pH values 6 and 9, and the dynamic binding capacity was estimated to 60 µg/ml resin.}}, author = {{Matos, Tiago and Queiroz, João A. and Bülow, Leif}}, issn = {{1099-1352}}, language = {{eng}}, number = {{4}}, pages = {{184--189}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Journal of Molecular Recognition}}, title = {{Plasmid DNA purification using a multimodal Chromatography resin}}, url = {{http://dx.doi.org/10.1002/jmr.2349}}, doi = {{10.1002/jmr.2349}}, volume = {{27}}, year = {{2014}}, }