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Evaluation of Drug Exposure and Metabolism in Locust and Zebrafish Brains Using Mass Spectrometry Imaging

Villacrez, Marvin LU ; Hellman, Karin LU ; Ono, Tatsuya LU ; Sugihara, Yutaka LU ; Rezeli, Melinda LU ; Ek, Fredrik LU ; Marko-Varga, Gyorgy LU and Olsson, Roger LU (2018) In ACS Chemical Neuroscience 9(8). p.1994-2000
Abstract

Studying how and where drugs are metabolized in the brain is challenging. In an entire organism, peripheral metabolism produces many of the same metabolites as those in the brain, and many of these metabolites can cross the blood-brain barrier from the periphery, thus making the relative contributions of hepatic and brain metabolism difficult to study in vivo. In addition, drugs and metabolites contained in ventricles and in the residual blood of capillaries in the brain may overestimate drugs' and metabolites' concentrations in the brain. In this study, we examine locusts and zebrafish using matrix assisted laser desorption ionization mass spectrometry imaging to study brain metabolism and distribution. These animal models are... (More)

Studying how and where drugs are metabolized in the brain is challenging. In an entire organism, peripheral metabolism produces many of the same metabolites as those in the brain, and many of these metabolites can cross the blood-brain barrier from the periphery, thus making the relative contributions of hepatic and brain metabolism difficult to study in vivo. In addition, drugs and metabolites contained in ventricles and in the residual blood of capillaries in the brain may overestimate drugs' and metabolites' concentrations in the brain. In this study, we examine locusts and zebrafish using matrix assisted laser desorption ionization mass spectrometry imaging to study brain metabolism and distribution. These animal models are cost-effective and ethically sound for initial drug development studies.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
blood-brain barrier, clozapine, drug metabolism, Locust, Mass spectrometry imaging, zebrafish
in
ACS Chemical Neuroscience
volume
9
issue
8
pages
7 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:29350027
  • scopus:85051668187
ISSN
1948-7193
DOI
10.1021/acschemneuro.7b00459
language
English
LU publication?
yes
id
423a3054-8ed1-4c55-915c-1b4bf3783d05
date added to LUP
2018-09-10 09:36:10
date last changed
2019-12-10 07:39:35
@article{423a3054-8ed1-4c55-915c-1b4bf3783d05,
  abstract     = {<p>Studying how and where drugs are metabolized in the brain is challenging. In an entire organism, peripheral metabolism produces many of the same metabolites as those in the brain, and many of these metabolites can cross the blood-brain barrier from the periphery, thus making the relative contributions of hepatic and brain metabolism difficult to study in vivo. In addition, drugs and metabolites contained in ventricles and in the residual blood of capillaries in the brain may overestimate drugs' and metabolites' concentrations in the brain. In this study, we examine locusts and zebrafish using matrix assisted laser desorption ionization mass spectrometry imaging to study brain metabolism and distribution. These animal models are cost-effective and ethically sound for initial drug development studies.</p>},
  author       = {Villacrez, Marvin and Hellman, Karin and Ono, Tatsuya and Sugihara, Yutaka and Rezeli, Melinda and Ek, Fredrik and Marko-Varga, Gyorgy and Olsson, Roger},
  issn         = {1948-7193},
  language     = {eng},
  month        = {08},
  number       = {8},
  pages        = {1994--2000},
  publisher    = {The American Chemical Society (ACS)},
  series       = {ACS Chemical Neuroscience},
  title        = {Evaluation of Drug Exposure and Metabolism in Locust and Zebrafish Brains Using Mass Spectrometry Imaging},
  url          = {http://dx.doi.org/10.1021/acschemneuro.7b00459},
  doi          = {10.1021/acschemneuro.7b00459},
  volume       = {9},
  year         = {2018},
}