Defensin-rich granules of human neutrophils: characterization of secretory properties
(2002) In Biochimica et Biophysica Acta 1591(1-3). p.29-35- Abstract
- The various granule subtypes of the human neutrophil differ in propensity for exocytosis. As a rule, granules formed at late stages of myelopoiesis have a higher secretory potential than granules formed in more immature myeloid cells. Neutrophils contain four closely related alpha-defensins, which are stored in a subset of azurophil granules. These defensin-rich azurophil granules (DRG) are formed later than defensin-poor azurophil granules, near the promyelocyte/myelocyte transition. In order to characterize the secretory properties of DRG, we developed a sensitive and accurate ELISA for detection of the neutrophil alpha-defensins HNP 1-3. This allowed us to quantify the exocytosis of alpha-defensins and markers of azurophil... (More)
- The various granule subtypes of the human neutrophil differ in propensity for exocytosis. As a rule, granules formed at late stages of myelopoiesis have a higher secretory potential than granules formed in more immature myeloid cells. Neutrophils contain four closely related alpha-defensins, which are stored in a subset of azurophil granules. These defensin-rich azurophil granules (DRG) are formed later than defensin-poor azurophil granules, near the promyelocyte/myelocyte transition. In order to characterize the secretory properties of DRG, we developed a sensitive and accurate ELISA for detection of the neutrophil alpha-defensins HNP 1-3. This allowed us to quantify the exocytosis of alpha-defensins and markers of azurophil (myeloperoxidase), specific (lactoferrin) and gelatinase (gelatinase) granules from neutrophils stimulated with different secretagogues. The release pattern of alpha-defensins correlated perfectly with the release of myeloperoxidase and showed no resemblance to the exocytosis of lactoferrin or gelatinase. This finding was substantiated through subcellular fractionation experiments. In conclusion, despite a distinct profile of biosynthesis, DRG are indistinguishable from defensin-poor azurophil granules with respect to exocytosis. Thus, in contrast to peroxidase-negative granules, azurophil granules display homogeneity in their availability for extracellular release. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1123808
- author
- Faurschou, Mikkel ; Sørensen, Ole E LU ; Johnsen, Anders H ; Askaa, Jon and Borregaard, Niels
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochimica et Biophysica Acta
- volume
- 1591
- issue
- 1-3
- pages
- 29 - 35
- publisher
- Elsevier
- external identifiers
-
- pmid:12183052
- scopus:0037135707
- ISSN
- 0006-3002
- DOI
- 10.1016/S0167-4889(02)00243-4
- language
- English
- LU publication?
- no
- id
- 4270e568-76ce-451e-bad7-da251006ca6f (old id 1123808)
- date added to LUP
- 2016-04-01 15:24:38
- date last changed
- 2022-04-22 07:36:45
@article{4270e568-76ce-451e-bad7-da251006ca6f,
abstract = {{The various granule subtypes of the human neutrophil differ in propensity for exocytosis. As a rule, granules formed at late stages of myelopoiesis have a higher secretory potential than granules formed in more immature myeloid cells. Neutrophils contain four closely related alpha-defensins, which are stored in a subset of azurophil granules. These defensin-rich azurophil granules (DRG) are formed later than defensin-poor azurophil granules, near the promyelocyte/myelocyte transition. In order to characterize the secretory properties of DRG, we developed a sensitive and accurate ELISA for detection of the neutrophil alpha-defensins HNP 1-3. This allowed us to quantify the exocytosis of alpha-defensins and markers of azurophil (myeloperoxidase), specific (lactoferrin) and gelatinase (gelatinase) granules from neutrophils stimulated with different secretagogues. The release pattern of alpha-defensins correlated perfectly with the release of myeloperoxidase and showed no resemblance to the exocytosis of lactoferrin or gelatinase. This finding was substantiated through subcellular fractionation experiments. In conclusion, despite a distinct profile of biosynthesis, DRG are indistinguishable from defensin-poor azurophil granules with respect to exocytosis. Thus, in contrast to peroxidase-negative granules, azurophil granules display homogeneity in their availability for extracellular release.}},
author = {{Faurschou, Mikkel and Sørensen, Ole E and Johnsen, Anders H and Askaa, Jon and Borregaard, Niels}},
issn = {{0006-3002}},
language = {{eng}},
number = {{1-3}},
pages = {{29--35}},
publisher = {{Elsevier}},
series = {{Biochimica et Biophysica Acta}},
title = {{Defensin-rich granules of human neutrophils: characterization of secretory properties}},
url = {{http://dx.doi.org/10.1016/S0167-4889(02)00243-4}},
doi = {{10.1016/S0167-4889(02)00243-4}},
volume = {{1591}},
year = {{2002}},
}