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Three-dimensional imaging of whole mouse models: comparing nondestructive X-ray phase-contrast micro-CT with cryotome-based planar epi-illumination imaging

Tapfer, A.; Bech, Martin LU ; Zanette, I.; Symvoulidis, P.; Stangl, S.; Multhoff, G.; Molls, M.; Ntziachristos, V. and Pfeiffer, F. (2014) In Journal of Microscopy 253(1). p.24-30
Abstract
In this study, we compare two evolving techniques for obtaining high-resolution 3D anatomical data of a mouse specimen. On the one hand, we investigate cryotome-based planar epi-illumination imaging (cryo-imaging). On the other hand, we examine X-ray phase-contrast micro-computed tomography (micro-CT) using synchrotron radiation. Cryo-imaging is a technique in which an electron multiplying charge coupled camera takes images of a cryo-frozen specimen during the sectioning process. Subsequent image alignment and virtual stacking result in volumetric data. X-ray phase-contrast imaging is based on the minute refraction of X-rays inside the specimen and features higher soft-tissue contrast than conventional, attenuation-based micro-CT. To... (More)
In this study, we compare two evolving techniques for obtaining high-resolution 3D anatomical data of a mouse specimen. On the one hand, we investigate cryotome-based planar epi-illumination imaging (cryo-imaging). On the other hand, we examine X-ray phase-contrast micro-computed tomography (micro-CT) using synchrotron radiation. Cryo-imaging is a technique in which an electron multiplying charge coupled camera takes images of a cryo-frozen specimen during the sectioning process. Subsequent image alignment and virtual stacking result in volumetric data. X-ray phase-contrast imaging is based on the minute refraction of X-rays inside the specimen and features higher soft-tissue contrast than conventional, attenuation-based micro-CT. To explore the potential of both techniques for studying whole mouse disease models, one mouse specimen was imaged using both techniques. Obtained data are compared visually and quantitatively, specifically with regard to the visibility of fine anatomical details. Internal structure of the mouse specimen is visible in great detail with both techniques and the study shows in particular that soft-tissue contrast is strongly enhanced in the X-ray phase images compared to the attenuation-based images. This identifies phase-contrast micro-CT as a powerful tool for the study of small animal disease models. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Computed tomography, planar epi-illumination imaging, X-ray phase, contrast
in
Journal of Microscopy
volume
253
issue
1
pages
24 - 30
publisher
Wiley-Blackwell
external identifiers
  • wos:000327994100003
  • scopus:84889683968
ISSN
0022-2720
DOI
10.1111/jmi.12094
language
English
LU publication?
yes
id
9b90b5e5-6b30-4133-aac1-00dba6398830 (old id 4273014)
date added to LUP
2014-02-10 12:24:06
date last changed
2017-02-26 03:43:37
@article{9b90b5e5-6b30-4133-aac1-00dba6398830,
  abstract     = {In this study, we compare two evolving techniques for obtaining high-resolution 3D anatomical data of a mouse specimen. On the one hand, we investigate cryotome-based planar epi-illumination imaging (cryo-imaging). On the other hand, we examine X-ray phase-contrast micro-computed tomography (micro-CT) using synchrotron radiation. Cryo-imaging is a technique in which an electron multiplying charge coupled camera takes images of a cryo-frozen specimen during the sectioning process. Subsequent image alignment and virtual stacking result in volumetric data. X-ray phase-contrast imaging is based on the minute refraction of X-rays inside the specimen and features higher soft-tissue contrast than conventional, attenuation-based micro-CT. To explore the potential of both techniques for studying whole mouse disease models, one mouse specimen was imaged using both techniques. Obtained data are compared visually and quantitatively, specifically with regard to the visibility of fine anatomical details. Internal structure of the mouse specimen is visible in great detail with both techniques and the study shows in particular that soft-tissue contrast is strongly enhanced in the X-ray phase images compared to the attenuation-based images. This identifies phase-contrast micro-CT as a powerful tool for the study of small animal disease models.},
  author       = {Tapfer, A. and Bech, Martin and Zanette, I. and Symvoulidis, P. and Stangl, S. and Multhoff, G. and Molls, M. and Ntziachristos, V. and Pfeiffer, F.},
  issn         = {0022-2720},
  keyword      = {Computed tomography,planar epi-illumination imaging,X-ray phase,contrast},
  language     = {eng},
  number       = {1},
  pages        = {24--30},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Microscopy},
  title        = {Three-dimensional imaging of whole mouse models: comparing nondestructive X-ray phase-contrast micro-CT with cryotome-based planar epi-illumination imaging},
  url          = {http://dx.doi.org/10.1111/jmi.12094},
  volume       = {253},
  year         = {2014},
}