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Chemically modified poly(2-hydroxyethyl methacrylate) cryogel for the adsorption of heparin.

La Spina, Rita LU ; Tripisciano, C; Mecca, T; Cunsolo, F; Weber, V and Mattiasson, Bo LU (2014) In Journal of Biomedical Materials Research. Part B - Applied Biomaterials 102(6). p.1207-1216
Abstract
Various clinical procedures, such as cardiovascular surgery or extracorporeal blood purification, involve systemic anticoagulation using heparin. High concentrations of circulating heparin require neutralization due to possible serious bleeding complications. The intravenous administration of the heparin antagonist protamine sulfate is routinely clinically performed, but is frequently associated with adverse reactions. Therefore, there is a need for a valid and safe alternative to achieve extracorporeal heparin removal from blood or plasma, such as a filter, a matrix, or an adsorbent. Here, we describe the development of a macroporous poly(2-hydroxyethyl methacrylate)-based monolithic cryogel functionalized with l-lysine (pHEMA-lys) and... (More)
Various clinical procedures, such as cardiovascular surgery or extracorporeal blood purification, involve systemic anticoagulation using heparin. High concentrations of circulating heparin require neutralization due to possible serious bleeding complications. The intravenous administration of the heparin antagonist protamine sulfate is routinely clinically performed, but is frequently associated with adverse reactions. Therefore, there is a need for a valid and safe alternative to achieve extracorporeal heparin removal from blood or plasma, such as a filter, a matrix, or an adsorbent. Here, we describe the development of a macroporous poly(2-hydroxyethyl methacrylate)-based monolithic cryogel functionalized with l-lysine (pHEMA-lys) and the characterization of its selective heparin adsorption. The maximum binding capacity was quantified in vitro using aqueous and serum solutions under static and dynamic conditions, and fresh human plasma under static conditions. The pHEMA-lys bound 40,500 IU and 32,500 IU heparin/g cryogel at the equilibrium in aqueous solution and 50% serum, respectively. In human plasma spiked with 100 IU/mL of heparin, the binding was still highly efficient (4330 IU/g cryogel after 30 min, i.e., 87% of the initial concentration). The cryogels showed good blood compatibility, as indicated by negligible adsorption of albumin, antithrombin III, and total protein, and may thus be suitable for extracorporeal heparin removal. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2014. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biomedical Materials Research. Part B - Applied Biomaterials
volume
102
issue
6
pages
1207 - 1216
publisher
John Wiley & Sons
external identifiers
  • wos:000339106300011
  • pmid:24425361
  • scopus:84904045631
ISSN
1552-4981
DOI
10.1002/jbm.b.33104
language
English
LU publication?
yes
id
8bc353d3-6120-44c3-8bcb-a411959efb6a (old id 4291432)
date added to LUP
2014-02-24 12:14:03
date last changed
2017-08-20 03:27:17
@article{8bc353d3-6120-44c3-8bcb-a411959efb6a,
  abstract     = {Various clinical procedures, such as cardiovascular surgery or extracorporeal blood purification, involve systemic anticoagulation using heparin. High concentrations of circulating heparin require neutralization due to possible serious bleeding complications. The intravenous administration of the heparin antagonist protamine sulfate is routinely clinically performed, but is frequently associated with adverse reactions. Therefore, there is a need for a valid and safe alternative to achieve extracorporeal heparin removal from blood or plasma, such as a filter, a matrix, or an adsorbent. Here, we describe the development of a macroporous poly(2-hydroxyethyl methacrylate)-based monolithic cryogel functionalized with l-lysine (pHEMA-lys) and the characterization of its selective heparin adsorption. The maximum binding capacity was quantified in vitro using aqueous and serum solutions under static and dynamic conditions, and fresh human plasma under static conditions. The pHEMA-lys bound 40,500 IU and 32,500 IU heparin/g cryogel at the equilibrium in aqueous solution and 50% serum, respectively. In human plasma spiked with 100 IU/mL of heparin, the binding was still highly efficient (4330 IU/g cryogel after 30 min, i.e., 87% of the initial concentration). The cryogels showed good blood compatibility, as indicated by negligible adsorption of albumin, antithrombin III, and total protein, and may thus be suitable for extracorporeal heparin removal. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2014.},
  author       = {La Spina, Rita and Tripisciano, C and Mecca, T and Cunsolo, F and Weber, V and Mattiasson, Bo},
  issn         = {1552-4981},
  language     = {eng},
  number       = {6},
  pages        = {1207--1216},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Biomedical Materials Research. Part B - Applied Biomaterials},
  title        = {Chemically modified poly(2-hydroxyethyl methacrylate) cryogel for the adsorption of heparin.},
  url          = {http://dx.doi.org/10.1002/jbm.b.33104},
  volume       = {102},
  year         = {2014},
}