Dynamic changes in dopamine neuron function after DNSP-11 treatment: Effects in vivo and increased ERK 1/2 phosphorylation in vitro.
(2014) In Peptides 54(Jan 7). p.1-8- Abstract
- Glial cell-line derived neurotrophic factor (GDNF) has demonstrated robust effects on dopamine (DA) neuron function and survival. A post-translational processing model of the human GDNF proprotein theorizes the formation of smaller, amidated peptide(s) from the proregion that exhibit neurobiological function, including an 11-amino-acid peptide named dopamine neuron stimulating peptide-11 (DNSP-11). A single treatment of DNSP-11 was delivered to the substantia nigra in the rat to investigate effects on DA-neuron function. Four weeks after treatment, potassium (K+) and d-amphetamine evoked DA release were studied in the striatum using microdialysis. There were no significant changes in DA-release after DNSP-11 treatment determined by... (More)
- Glial cell-line derived neurotrophic factor (GDNF) has demonstrated robust effects on dopamine (DA) neuron function and survival. A post-translational processing model of the human GDNF proprotein theorizes the formation of smaller, amidated peptide(s) from the proregion that exhibit neurobiological function, including an 11-amino-acid peptide named dopamine neuron stimulating peptide-11 (DNSP-11). A single treatment of DNSP-11 was delivered to the substantia nigra in the rat to investigate effects on DA-neuron function. Four weeks after treatment, potassium (K+) and d-amphetamine evoked DA release were studied in the striatum using microdialysis. There were no significant changes in DA-release after DNSP-11 treatment determined by microdialysis. Dopamine release was further examined in discrete regions of the striatum using high-speed chronoamperometry at 1-, 2-, and 4-weeks after DNSP-11 treatment. Two weeks after DNSP-11 treatment, potassium-evoked DA release was increased in specific subregions of the striatum. However, spontaneous locomotor activity was unchanged by DNSP-11 treatment. In addition, we show that a single treatment of DNSP-11 in the MN9D dopaminergic neuronal cell line results in phosphorylation of ERK1/2, which suggests a novel cellular mechanism responsible for increases in DA function. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4291722
- author
- Fuqua, Joshua L ; Littrell, Ofelia M ; Lundblad, Martin LU ; Turchan-Cholewo, Jadwiga ; Abdelmoti, Lina G ; Galperin, Emilia ; Bradley, Luke H ; Cass, Wayne A ; Gash, Don M and Gerhardt, Greg A
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Peptides
- volume
- 54
- issue
- Jan 7
- pages
- 1 - 8
- publisher
- Elsevier
- external identifiers
-
- pmid:24406899
- wos:000334508400001
- scopus:84893128670
- pmid:24406899
- ISSN
- 1873-5169
- DOI
- 10.1016/j.peptides.2013.12.007
- language
- English
- LU publication?
- yes
- id
- 5b3434ad-fd3f-4ecd-8a55-22130b891c85 (old id 4291722)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24406899?dopt=Abstract
- date added to LUP
- 2016-04-01 10:00:15
- date last changed
- 2022-02-17 05:39:42
@article{5b3434ad-fd3f-4ecd-8a55-22130b891c85,
abstract = {{Glial cell-line derived neurotrophic factor (GDNF) has demonstrated robust effects on dopamine (DA) neuron function and survival. A post-translational processing model of the human GDNF proprotein theorizes the formation of smaller, amidated peptide(s) from the proregion that exhibit neurobiological function, including an 11-amino-acid peptide named dopamine neuron stimulating peptide-11 (DNSP-11). A single treatment of DNSP-11 was delivered to the substantia nigra in the rat to investigate effects on DA-neuron function. Four weeks after treatment, potassium (K+) and d-amphetamine evoked DA release were studied in the striatum using microdialysis. There were no significant changes in DA-release after DNSP-11 treatment determined by microdialysis. Dopamine release was further examined in discrete regions of the striatum using high-speed chronoamperometry at 1-, 2-, and 4-weeks after DNSP-11 treatment. Two weeks after DNSP-11 treatment, potassium-evoked DA release was increased in specific subregions of the striatum. However, spontaneous locomotor activity was unchanged by DNSP-11 treatment. In addition, we show that a single treatment of DNSP-11 in the MN9D dopaminergic neuronal cell line results in phosphorylation of ERK1/2, which suggests a novel cellular mechanism responsible for increases in DA function.}},
author = {{Fuqua, Joshua L and Littrell, Ofelia M and Lundblad, Martin and Turchan-Cholewo, Jadwiga and Abdelmoti, Lina G and Galperin, Emilia and Bradley, Luke H and Cass, Wayne A and Gash, Don M and Gerhardt, Greg A}},
issn = {{1873-5169}},
language = {{eng}},
number = {{Jan 7}},
pages = {{1--8}},
publisher = {{Elsevier}},
series = {{Peptides}},
title = {{Dynamic changes in dopamine neuron function after DNSP-11 treatment: Effects in vivo and increased ERK 1/2 phosphorylation in vitro.}},
url = {{http://dx.doi.org/10.1016/j.peptides.2013.12.007}},
doi = {{10.1016/j.peptides.2013.12.007}},
volume = {{54}},
year = {{2014}},
}