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Robust isolation of malignant plasma cells in multiple myeloma.

Frigyesi, Ildiko LU ; Adolfsson, Jörgen LU ; Ali, Mina LU ; Christophersen, Mikael Kronborg LU ; Johnsson, Ellinor LU ; Turesson, Ingemar LU ; Gullberg, Urban LU ; Hansson, Markus LU and Nilsson, Björn LU (2014) In Blood 123(9). p.1336-1340
Abstract
Molecular characterization of malignant plasma cells is increasingly important for diagnostic and therapeutic stratification in multiple myeloma (MM). However, the malignant plasma cells represent a relatively small subset of bone marrow cells, and need to be enriched prior to analysis. Currently, the cell surface marker CD138 (SDC1) is used for this enrichment, but has an important limitation in that its expression decreases rapidly after sampling. Seeking alternatives to CD138, we performed a computational screen for myeloma plasma cell markers and evaluated seven candidates systematically. Our results conclusively show that the markers CD319 (SLAMF7/CS1) and CD269 (TNFRSF17/BCMA) are considerably more robust than CD138, and enable... (More)
Molecular characterization of malignant plasma cells is increasingly important for diagnostic and therapeutic stratification in multiple myeloma (MM). However, the malignant plasma cells represent a relatively small subset of bone marrow cells, and need to be enriched prior to analysis. Currently, the cell surface marker CD138 (SDC1) is used for this enrichment, but has an important limitation in that its expression decreases rapidly after sampling. Seeking alternatives to CD138, we performed a computational screen for myeloma plasma cell markers and evaluated seven candidates systematically. Our results conclusively show that the markers CD319 (SLAMF7/CS1) and CD269 (TNFRSF17/BCMA) are considerably more robust than CD138, and enable isolation of myeloma plasma cells under more diverse conditions, including in samples that have been delayed or frozen. Our results form the basis of improved procedures for characterizing cases of multiple myeloma in clinical practice. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Blood
volume
123
issue
9
pages
1336 - 1340
publisher
American Society of Hematology
external identifiers
  • pmid:24385542
  • wos:000335839300016
  • scopus:84899692195
ISSN
1528-0020
DOI
10.1182/blood-2013-09-529800
language
English
LU publication?
yes
id
c3e71216-e4d1-436a-8943-862eed761439 (old id 4292197)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24385542?dopt=Abstract
date added to LUP
2014-02-06 20:38:42
date last changed
2017-11-19 03:07:53
@article{c3e71216-e4d1-436a-8943-862eed761439,
  abstract     = {Molecular characterization of malignant plasma cells is increasingly important for diagnostic and therapeutic stratification in multiple myeloma (MM). However, the malignant plasma cells represent a relatively small subset of bone marrow cells, and need to be enriched prior to analysis. Currently, the cell surface marker CD138 (SDC1) is used for this enrichment, but has an important limitation in that its expression decreases rapidly after sampling. Seeking alternatives to CD138, we performed a computational screen for myeloma plasma cell markers and evaluated seven candidates systematically. Our results conclusively show that the markers CD319 (SLAMF7/CS1) and CD269 (TNFRSF17/BCMA) are considerably more robust than CD138, and enable isolation of myeloma plasma cells under more diverse conditions, including in samples that have been delayed or frozen. Our results form the basis of improved procedures for characterizing cases of multiple myeloma in clinical practice.},
  author       = {Frigyesi, Ildiko and Adolfsson, Jörgen and Ali, Mina and Christophersen, Mikael Kronborg and Johnsson, Ellinor and Turesson, Ingemar and Gullberg, Urban and Hansson, Markus and Nilsson, Björn},
  issn         = {1528-0020},
  language     = {eng},
  number       = {9},
  pages        = {1336--1340},
  publisher    = {American Society of Hematology},
  series       = {Blood},
  title        = {Robust isolation of malignant plasma cells in multiple myeloma.},
  url          = {http://dx.doi.org/10.1182/blood-2013-09-529800},
  volume       = {123},
  year         = {2014},
}